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Cattle NK Cell Heterogeneity and the Influence of MHC Class I.

Allan AJ, Sanderson ND, Gubbins S, Ellis SA, Hammond JA - J. Immunol. (2015)

Bottom Line: Cytokine stimulation differentially influenced the transcription of five receptors at the cell population level.Using dilution cultures, we found that a further seven receptors were differentially transcribed, including five predicted to recognize MHC class I.Moreover, there was a statistically significant reduction in killer cell lectin-like receptor mRNA expression between cultures with different CD2 phenotypes and from animals with different MHC class I haplotypes.

View Article: PubMed Central - PubMed

Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.

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Related in: MedlinePlus

The frequency of dilution cultures transcribing NK cell receptors correlates with MHC class I genotype (A) and CD2 expression (B). Gene transcription is displayed as a normalized percentage of the total number of cultures within each population. Samples in which expression was mixed and genes were transcribed by all the dilution cultures were omitted. Statistical significance was determined using Fisher’s exact tests. *p < 0.05.
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fig05: The frequency of dilution cultures transcribing NK cell receptors correlates with MHC class I genotype (A) and CD2 expression (B). Gene transcription is displayed as a normalized percentage of the total number of cultures within each population. Samples in which expression was mixed and genes were transcribed by all the dilution cultures were omitted. Statistical significance was determined using Fisher’s exact tests. *p < 0.05.

Mentions: Having established NK cell receptor transcriptional variability within the dilution cultures, we examined whether there was any association between receptor transcription and NK cell phenotype or genotype. The expression of CD2 is an important differentiator of functional NK cell subsets in cattle (26). Therefore, we grouped the data from each dilution culture by CD2 expression and MHC class I genotype. Besides the significant association of CD2 transcription with expression, KLRC1*2-7 and KIR3DXL1 were significantly more likely to be present on the CD2high NK cell subset regardless of MHC class I genotype (p < 0.05) (Fig. 5). The transcription of more inhibitory receptors correlates with the stronger inhibition of CD2high NK cells compared with CD2low (26).


Cattle NK Cell Heterogeneity and the Influence of MHC Class I.

Allan AJ, Sanderson ND, Gubbins S, Ellis SA, Hammond JA - J. Immunol. (2015)

The frequency of dilution cultures transcribing NK cell receptors correlates with MHC class I genotype (A) and CD2 expression (B). Gene transcription is displayed as a normalized percentage of the total number of cultures within each population. Samples in which expression was mixed and genes were transcribed by all the dilution cultures were omitted. Statistical significance was determined using Fisher’s exact tests. *p < 0.05.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4543905&req=5

fig05: The frequency of dilution cultures transcribing NK cell receptors correlates with MHC class I genotype (A) and CD2 expression (B). Gene transcription is displayed as a normalized percentage of the total number of cultures within each population. Samples in which expression was mixed and genes were transcribed by all the dilution cultures were omitted. Statistical significance was determined using Fisher’s exact tests. *p < 0.05.
Mentions: Having established NK cell receptor transcriptional variability within the dilution cultures, we examined whether there was any association between receptor transcription and NK cell phenotype or genotype. The expression of CD2 is an important differentiator of functional NK cell subsets in cattle (26). Therefore, we grouped the data from each dilution culture by CD2 expression and MHC class I genotype. Besides the significant association of CD2 transcription with expression, KLRC1*2-7 and KIR3DXL1 were significantly more likely to be present on the CD2high NK cell subset regardless of MHC class I genotype (p < 0.05) (Fig. 5). The transcription of more inhibitory receptors correlates with the stronger inhibition of CD2high NK cells compared with CD2low (26).

Bottom Line: Cytokine stimulation differentially influenced the transcription of five receptors at the cell population level.Using dilution cultures, we found that a further seven receptors were differentially transcribed, including five predicted to recognize MHC class I.Moreover, there was a statistically significant reduction in killer cell lectin-like receptor mRNA expression between cultures with different CD2 phenotypes and from animals with different MHC class I haplotypes.

View Article: PubMed Central - PubMed

Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.

Show MeSH
Related in: MedlinePlus