Cattle NK Cell Heterogeneity and the Influence of MHC Class I.
Bottom Line: Cytokine stimulation differentially influenced the transcription of five receptors at the cell population level.Using dilution cultures, we found that a further seven receptors were differentially transcribed, including five predicted to recognize MHC class I.Moreover, there was a statistically significant reduction in killer cell lectin-like receptor mRNA expression between cultures with different CD2 phenotypes and from animals with different MHC class I haplotypes.
Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.Show MeSH
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Mentions: Having established NK cell receptor transcriptional variability within the dilution cultures, we examined whether there was any association between receptor transcription and NK cell phenotype or genotype. The expression of CD2 is an important differentiator of functional NK cell subsets in cattle (26). Therefore, we grouped the data from each dilution culture by CD2 expression and MHC class I genotype. Besides the significant association of CD2 transcription with expression, KLRC1*2-7 and KIR3DXL1 were significantly more likely to be present on the CD2high NK cell subset regardless of MHC class I genotype (p < 0.05) (Fig. 5). The transcription of more inhibitory receptors correlates with the stronger inhibition of CD2high NK cells compared with CD2low (26).
Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.