Cattle NK Cell Heterogeneity and the Influence of MHC Class I.
Bottom Line: Cytokine stimulation differentially influenced the transcription of five receptors at the cell population level.Using dilution cultures, we found that a further seven receptors were differentially transcribed, including five predicted to recognize MHC class I.Moreover, there was a statistically significant reduction in killer cell lectin-like receptor mRNA expression between cultures with different CD2 phenotypes and from animals with different MHC class I haplotypes.
Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.Show MeSH
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Mentions: Transcription of the same 25 NK cell markers and receptors previously examined in ex vivo NK cells was determined (Fig. 4). A majority of the CKM genes were differentially transcribed; CD2, CD8α, CD8β, CD25, and NCR1 were all variably present between dilution cultures from several individual animals. The variable pattern of TIM-3 and 2B4 transcription was generally consistent between the dilution cultures and ex vivo NK cells from the same animals. Within the KLR group, mRNA for KLRC1*2-7, KLRC2*2, KLRD*1, and KLRD*2 was ubiquitously present at the population level but variably transcribed between the dilution cultures. Transcription of the KLRA alleles was consistent with the NK cell population data, with animals heterozygous for both allele lineages showing variable transcription patterns. As predicted from ex vivo NK cell populations, KIR3DXL6 was variably transcribed between dilution cultures, as was KIR3DXL1 and, to a more limited extent, the KIR group containing KIR3DXL3/5/7.
Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.