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Cattle NK Cell Heterogeneity and the Influence of MHC Class I.

Allan AJ, Sanderson ND, Gubbins S, Ellis SA, Hammond JA - J. Immunol. (2015)

Bottom Line: Cytokine stimulation differentially influenced the transcription of five receptors at the cell population level.Using dilution cultures, we found that a further seven receptors were differentially transcribed, including five predicted to recognize MHC class I.Moreover, there was a statistically significant reduction in killer cell lectin-like receptor mRNA expression between cultures with different CD2 phenotypes and from animals with different MHC class I haplotypes.

View Article: PubMed Central - PubMed

Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.

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Related in: MedlinePlus

A summary of NK receptor transcription patterns from ex vivo, in vitro stimulated, and limiting dilution culture NK cells. Immediately ex vivo (NCR1+), NK cells that were stimulated in vitro in IL-2, IL-12, and IL-18 or IL-15 for 7 d (cytokine stimulated), and limiting dilution cultures (dilution cultures) from each animal are shown. Each square displays the percentage of transcription of that gene for that sample.
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fig04: A summary of NK receptor transcription patterns from ex vivo, in vitro stimulated, and limiting dilution culture NK cells. Immediately ex vivo (NCR1+), NK cells that were stimulated in vitro in IL-2, IL-12, and IL-18 or IL-15 for 7 d (cytokine stimulated), and limiting dilution cultures (dilution cultures) from each animal are shown. Each square displays the percentage of transcription of that gene for that sample.

Mentions: Transcription of the same 25 NK cell markers and receptors previously examined in ex vivo NK cells was determined (Fig. 4). A majority of the CKM genes were differentially transcribed; CD2, CD8α, CD8β, CD25, and NCR1 were all variably present between dilution cultures from several individual animals. The variable pattern of TIM-3 and 2B4 transcription was generally consistent between the dilution cultures and ex vivo NK cells from the same animals. Within the KLR group, mRNA for KLRC1*2-7, KLRC2*2, KLRD*1, and KLRD*2 was ubiquitously present at the population level but variably transcribed between the dilution cultures. Transcription of the KLRA alleles was consistent with the NK cell population data, with animals heterozygous for both allele lineages showing variable transcription patterns. As predicted from ex vivo NK cell populations, KIR3DXL6 was variably transcribed between dilution cultures, as was KIR3DXL1 and, to a more limited extent, the KIR group containing KIR3DXL3/5/7.


Cattle NK Cell Heterogeneity and the Influence of MHC Class I.

Allan AJ, Sanderson ND, Gubbins S, Ellis SA, Hammond JA - J. Immunol. (2015)

A summary of NK receptor transcription patterns from ex vivo, in vitro stimulated, and limiting dilution culture NK cells. Immediately ex vivo (NCR1+), NK cells that were stimulated in vitro in IL-2, IL-12, and IL-18 or IL-15 for 7 d (cytokine stimulated), and limiting dilution cultures (dilution cultures) from each animal are shown. Each square displays the percentage of transcription of that gene for that sample.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4543905&req=5

fig04: A summary of NK receptor transcription patterns from ex vivo, in vitro stimulated, and limiting dilution culture NK cells. Immediately ex vivo (NCR1+), NK cells that were stimulated in vitro in IL-2, IL-12, and IL-18 or IL-15 for 7 d (cytokine stimulated), and limiting dilution cultures (dilution cultures) from each animal are shown. Each square displays the percentage of transcription of that gene for that sample.
Mentions: Transcription of the same 25 NK cell markers and receptors previously examined in ex vivo NK cells was determined (Fig. 4). A majority of the CKM genes were differentially transcribed; CD2, CD8α, CD8β, CD25, and NCR1 were all variably present between dilution cultures from several individual animals. The variable pattern of TIM-3 and 2B4 transcription was generally consistent between the dilution cultures and ex vivo NK cells from the same animals. Within the KLR group, mRNA for KLRC1*2-7, KLRC2*2, KLRD*1, and KLRD*2 was ubiquitously present at the population level but variably transcribed between the dilution cultures. Transcription of the KLRA alleles was consistent with the NK cell population data, with animals heterozygous for both allele lineages showing variable transcription patterns. As predicted from ex vivo NK cell populations, KIR3DXL6 was variably transcribed between dilution cultures, as was KIR3DXL1 and, to a more limited extent, the KIR group containing KIR3DXL3/5/7.

Bottom Line: Cytokine stimulation differentially influenced the transcription of five receptors at the cell population level.Using dilution cultures, we found that a further seven receptors were differentially transcribed, including five predicted to recognize MHC class I.Moreover, there was a statistically significant reduction in killer cell lectin-like receptor mRNA expression between cultures with different CD2 phenotypes and from animals with different MHC class I haplotypes.

View Article: PubMed Central - PubMed

Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.

Show MeSH
Related in: MedlinePlus