Cattle NK Cell Heterogeneity and the Influence of MHC Class I.
Bottom Line: Cytokine stimulation differentially influenced the transcription of five receptors at the cell population level.Using dilution cultures, we found that a further seven receptors were differentially transcribed, including five predicted to recognize MHC class I.Moreover, there was a statistically significant reduction in killer cell lectin-like receptor mRNA expression between cultures with different CD2 phenotypes and from animals with different MHC class I haplotypes.
Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.Show MeSH
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Mentions: Although a majority of the dilution cultures appeared to be from a single-cell origin, with a single phenotype determined by expression of NCR1 and CD2 (Fig. 3A–C), one had a mixed CD2 phenotype (Fig. 3D) and two had mixed NCR1 phenotypes (Fig. 3E, 3F). This finding could indicate a multiple-cell origin or the transient expression of NCR1 and CD2. As further limiting dilution was not possible, all 66 populations are referred to as dilution cultures rather than clones for the remainder of this article.
Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.