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Cattle NK Cell Heterogeneity and the Influence of MHC Class I.

Allan AJ, Sanderson ND, Gubbins S, Ellis SA, Hammond JA - J. Immunol. (2015)

Bottom Line: Cytokine stimulation differentially influenced the transcription of five receptors at the cell population level.Using dilution cultures, we found that a further seven receptors were differentially transcribed, including five predicted to recognize MHC class I.Moreover, there was a statistically significant reduction in killer cell lectin-like receptor mRNA expression between cultures with different CD2 phenotypes and from animals with different MHC class I haplotypes.

View Article: PubMed Central - PubMed

Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.

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Individual dilution cultures have variable CD2 and NCR1 expression. Representative plots from six dilution cultures showing NCR1 and CD2 cell surface expression that was assessed by flow cytometry for each of the 66 limiting dilution cultures. Quadrants were set based on isotype matched control mAbs as described in Materials and Methods, with percentages shown in each quadrant.
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fig03: Individual dilution cultures have variable CD2 and NCR1 expression. Representative plots from six dilution cultures showing NCR1 and CD2 cell surface expression that was assessed by flow cytometry for each of the 66 limiting dilution cultures. Quadrants were set based on isotype matched control mAbs as described in Materials and Methods, with percentages shown in each quadrant.

Mentions: Although a majority of the dilution cultures appeared to be from a single-cell origin, with a single phenotype determined by expression of NCR1 and CD2 (Fig. 3A–C), one had a mixed CD2 phenotype (Fig. 3D) and two had mixed NCR1 phenotypes (Fig. 3E, 3F). This finding could indicate a multiple-cell origin or the transient expression of NCR1 and CD2. As further limiting dilution was not possible, all 66 populations are referred to as dilution cultures rather than clones for the remainder of this article.


Cattle NK Cell Heterogeneity and the Influence of MHC Class I.

Allan AJ, Sanderson ND, Gubbins S, Ellis SA, Hammond JA - J. Immunol. (2015)

Individual dilution cultures have variable CD2 and NCR1 expression. Representative plots from six dilution cultures showing NCR1 and CD2 cell surface expression that was assessed by flow cytometry for each of the 66 limiting dilution cultures. Quadrants were set based on isotype matched control mAbs as described in Materials and Methods, with percentages shown in each quadrant.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4543905&req=5

fig03: Individual dilution cultures have variable CD2 and NCR1 expression. Representative plots from six dilution cultures showing NCR1 and CD2 cell surface expression that was assessed by flow cytometry for each of the 66 limiting dilution cultures. Quadrants were set based on isotype matched control mAbs as described in Materials and Methods, with percentages shown in each quadrant.
Mentions: Although a majority of the dilution cultures appeared to be from a single-cell origin, with a single phenotype determined by expression of NCR1 and CD2 (Fig. 3A–C), one had a mixed CD2 phenotype (Fig. 3D) and two had mixed NCR1 phenotypes (Fig. 3E, 3F). This finding could indicate a multiple-cell origin or the transient expression of NCR1 and CD2. As further limiting dilution was not possible, all 66 populations are referred to as dilution cultures rather than clones for the remainder of this article.

Bottom Line: Cytokine stimulation differentially influenced the transcription of five receptors at the cell population level.Using dilution cultures, we found that a further seven receptors were differentially transcribed, including five predicted to recognize MHC class I.Moreover, there was a statistically significant reduction in killer cell lectin-like receptor mRNA expression between cultures with different CD2 phenotypes and from animals with different MHC class I haplotypes.

View Article: PubMed Central - PubMed

Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.

Show MeSH
Related in: MedlinePlus