Cattle NK Cell Heterogeneity and the Influence of MHC Class I.
Bottom Line: Cytokine stimulation differentially influenced the transcription of five receptors at the cell population level.Using dilution cultures, we found that a further seven receptors were differentially transcribed, including five predicted to recognize MHC class I.Moreover, there was a statistically significant reduction in killer cell lectin-like receptor mRNA expression between cultures with different CD2 phenotypes and from animals with different MHC class I haplotypes.
Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.Show MeSH
Related in: MedlinePlus
Mentions: As this study exploited a specially bred herd of MHC homozygous Holstein-Friesian cattle, it was important to establish that NK cells isolated from these animals were not substantively different from those used in previous studies (25, 26). Cattle NK cells are defined as NCR1+, with variable CD2 and CD8α expression (25, 26). We determined the cell surface expression of CD2 and CD8α on ex vivo and in vitro stimulated NCR1+ PBMCs from 14 MHC homozygous cattle. These animals represented three different haplotypes, four A18 containing only gene 6, four A14 containing a gene 1, 2 and 4 and six A31 containing a gene 1 and 2 but with different alleles to the A14. In all the animals, ∼80% of NCR1+ cells were CD2high ex vivo and CD2 expression was reduced nonsignificantly (p = 0.27) after stimulation with IL-2 (Fig. 1). In contrast, CD8α expression increased in vitro under IL-2 when compared with ex vivo cells (p < 0.001) (Fig. 1), as has been shown with other cytokines (25). These NK phenotypes are consistent with previously published data showing that NK cells from these homozygous cattle have a broadly conventional phenotype and are responsive to cytokine stimulation.
Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.