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Effect of K+ and Ca2+ on the indole-3-acetic acid- and fusicoccin-induced growth and membrane potential in maize coleoptile cells.

Siemieniuk A, Karcz W - AoB Plants (2015)

Bottom Line: It was also found that in parenchymal cells of maize coleoptile segments, membrane potential (Em) was strongly affected by the medium K(+), independently of Ca(2+).However, lack of Ca(2+) in the incubation medium significantly reduced the IAA- and FC-induced membrane potential hyperpolarization.Verapamil did not change either the Em of parenchymal cells incubated in the control medium or the IAA- and FC-induced membrane hyperpolarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Physiology, Faculty of Biology and Environmental Protection, University of Silesia, Jagiellońska 28, 40-032 Katowice, Silesia, Poland.

No MeSH data available.


Related in: MedlinePlus

Effect of K+ and Ca2+ channel blockers (TEA-Cl and verapamil, respectively) on the IAA-induced growth rate (μm s−1 cm−1) of maize coleoptile segments. Coleoptile segments were first preincubated for over 1 h in a control medium, whereupon channel blockers were added. At 2 h, IAA was added to the incubation medium, at a final concentration of 10 µM. Inset on the right shows the total elongation growth, presented in a bar graph, calculated as the sum of extensions between 120 and 600 min of the experiment. Values are the mean of at least nine independent experiments. Bars indicate mean ± SE. Mean values followed by the same letter are not significantly different from each other according to the LSD test (P < 0.05).
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PLV070F4: Effect of K+ and Ca2+ channel blockers (TEA-Cl and verapamil, respectively) on the IAA-induced growth rate (μm s−1 cm−1) of maize coleoptile segments. Coleoptile segments were first preincubated for over 1 h in a control medium, whereupon channel blockers were added. At 2 h, IAA was added to the incubation medium, at a final concentration of 10 µM. Inset on the right shows the total elongation growth, presented in a bar graph, calculated as the sum of extensions between 120 and 600 min of the experiment. Values are the mean of at least nine independent experiments. Bars indicate mean ± SE. Mean values followed by the same letter are not significantly different from each other according to the LSD test (P < 0.05).

Mentions: Figure 4 shows the effects of K+ and Ca2+ channel blockers (TEA-Cl and verapamil, respectively) on IAA-induced growth of maize coleoptile segments incubated in the presence of 1 mM K+ and 0.1 mM Ca2+. The segments were first preincubated for 1 h before the blockers were added. At 2 h, IAA was applied to the incubation medium. Data in Fig. 4 indicate that TEA-Cl, added at a final concentration of 30 mM, significantly decreased the growth of IAA-incubated coleoptile segments (F1,17 = 46.61, P < 0.01) (2935.3 ± 324.6 µm cm−1; mean ± SE, n = 9) by 59 %. TEA-Cl added alone 1 h after start of the experiment diminished growth (F1,16 = 24.18, P < 0.01) by 40 % as compared with control (1494.7 ± 146.4 µm cm−1; mean ± SE, n = 9) (Fig. 4, inset). Addition of verapamil, at a final concentration of 50 µM, in the same time protocol as TEA-Cl, increased IAA-induced growth by 7 % (statistically not significant, F1,16 = 0.01, P = 0.76) (Fig. 4, inset). As presented in Fig. 5, FC added to incubation medium containing TEA-Cl enhanced the growth of coleoptile segments (F1,18 = 39.81, P < 0.01) to a level 45 % lower than FC alone (2998.6 ± 265.0 µm cm−1; mean ± SE, n = 10). Verapamil added in the same way as TEA-Cl did not change elongation growth in response to FC (F1,17 = 1.52, P = 0.23).Figure 4.


Effect of K+ and Ca2+ on the indole-3-acetic acid- and fusicoccin-induced growth and membrane potential in maize coleoptile cells.

Siemieniuk A, Karcz W - AoB Plants (2015)

Effect of K+ and Ca2+ channel blockers (TEA-Cl and verapamil, respectively) on the IAA-induced growth rate (μm s−1 cm−1) of maize coleoptile segments. Coleoptile segments were first preincubated for over 1 h in a control medium, whereupon channel blockers were added. At 2 h, IAA was added to the incubation medium, at a final concentration of 10 µM. Inset on the right shows the total elongation growth, presented in a bar graph, calculated as the sum of extensions between 120 and 600 min of the experiment. Values are the mean of at least nine independent experiments. Bars indicate mean ± SE. Mean values followed by the same letter are not significantly different from each other according to the LSD test (P < 0.05).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4543891&req=5

PLV070F4: Effect of K+ and Ca2+ channel blockers (TEA-Cl and verapamil, respectively) on the IAA-induced growth rate (μm s−1 cm−1) of maize coleoptile segments. Coleoptile segments were first preincubated for over 1 h in a control medium, whereupon channel blockers were added. At 2 h, IAA was added to the incubation medium, at a final concentration of 10 µM. Inset on the right shows the total elongation growth, presented in a bar graph, calculated as the sum of extensions between 120 and 600 min of the experiment. Values are the mean of at least nine independent experiments. Bars indicate mean ± SE. Mean values followed by the same letter are not significantly different from each other according to the LSD test (P < 0.05).
Mentions: Figure 4 shows the effects of K+ and Ca2+ channel blockers (TEA-Cl and verapamil, respectively) on IAA-induced growth of maize coleoptile segments incubated in the presence of 1 mM K+ and 0.1 mM Ca2+. The segments were first preincubated for 1 h before the blockers were added. At 2 h, IAA was applied to the incubation medium. Data in Fig. 4 indicate that TEA-Cl, added at a final concentration of 30 mM, significantly decreased the growth of IAA-incubated coleoptile segments (F1,17 = 46.61, P < 0.01) (2935.3 ± 324.6 µm cm−1; mean ± SE, n = 9) by 59 %. TEA-Cl added alone 1 h after start of the experiment diminished growth (F1,16 = 24.18, P < 0.01) by 40 % as compared with control (1494.7 ± 146.4 µm cm−1; mean ± SE, n = 9) (Fig. 4, inset). Addition of verapamil, at a final concentration of 50 µM, in the same time protocol as TEA-Cl, increased IAA-induced growth by 7 % (statistically not significant, F1,16 = 0.01, P = 0.76) (Fig. 4, inset). As presented in Fig. 5, FC added to incubation medium containing TEA-Cl enhanced the growth of coleoptile segments (F1,18 = 39.81, P < 0.01) to a level 45 % lower than FC alone (2998.6 ± 265.0 µm cm−1; mean ± SE, n = 10). Verapamil added in the same way as TEA-Cl did not change elongation growth in response to FC (F1,17 = 1.52, P = 0.23).Figure 4.

Bottom Line: It was also found that in parenchymal cells of maize coleoptile segments, membrane potential (Em) was strongly affected by the medium K(+), independently of Ca(2+).However, lack of Ca(2+) in the incubation medium significantly reduced the IAA- and FC-induced membrane potential hyperpolarization.Verapamil did not change either the Em of parenchymal cells incubated in the control medium or the IAA- and FC-induced membrane hyperpolarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Physiology, Faculty of Biology and Environmental Protection, University of Silesia, Jagiellońska 28, 40-032 Katowice, Silesia, Poland.

No MeSH data available.


Related in: MedlinePlus