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Effect of K+ and Ca2+ on the indole-3-acetic acid- and fusicoccin-induced growth and membrane potential in maize coleoptile cells.

Siemieniuk A, Karcz W - AoB Plants (2015)

Bottom Line: It was also found that in parenchymal cells of maize coleoptile segments, membrane potential (Em) was strongly affected by the medium K(+), independently of Ca(2+).However, lack of Ca(2+) in the incubation medium significantly reduced the IAA- and FC-induced membrane potential hyperpolarization.Verapamil did not change either the Em of parenchymal cells incubated in the control medium or the IAA- and FC-induced membrane hyperpolarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Physiology, Faculty of Biology and Environmental Protection, University of Silesia, Jagiellońska 28, 40-032 Katowice, Silesia, Poland.

No MeSH data available.


Related in: MedlinePlus

Effect of K+ and Ca2+ ions on the endogenous growth rate (growth without growth substances) of maize coleoptile segments. (A) Growth rate (µm s−1 cm−1) of coleoptile segments incubated in the medium with 0.0 mM K+ and Ca2+ at 0.0, 0.1 and 1.0 mM. (B) As in A but with 1.0 mM K+. (C) As in A and B but with 10 mM K+ in the medium. The growth rate of five coleoptile segments (10 mm in length) was recorded for 10 h in an intensively aerated incubation medium (5 mL segment−1) by means of an angular position transducer (TWK Electronic). Inset shows total elongation growth calculated as the sum of extensions measured in 3-min intervals for 10 h. All curves are mean values of at least eight independent measurements. Bars indicate ±SE.
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PLV070F1: Effect of K+ and Ca2+ ions on the endogenous growth rate (growth without growth substances) of maize coleoptile segments. (A) Growth rate (µm s−1 cm−1) of coleoptile segments incubated in the medium with 0.0 mM K+ and Ca2+ at 0.0, 0.1 and 1.0 mM. (B) As in A but with 1.0 mM K+. (C) As in A and B but with 10 mM K+ in the medium. The growth rate of five coleoptile segments (10 mm in length) was recorded for 10 h in an intensively aerated incubation medium (5 mL segment−1) by means of an angular position transducer (TWK Electronic). Inset shows total elongation growth calculated as the sum of extensions measured in 3-min intervals for 10 h. All curves are mean values of at least eight independent measurements. Bars indicate ±SE.

Mentions: The effects of K+ (0.0, 1.0 and 10 mM) and Ca2+ (0.0, 0.1 and 1 mM) on the growth rate of maize coleoptile segments incubated in control medium (endogenous growth) are shown in Fig. 1. On the basis of growth rate responses, the total elongation growth (calculated as the sum of extensions from 3-min intervals) of coleoptile segments was also obtained (Fig. 1, insets). Taking these data into account, it should be stated that in media without K+, Ca2+ at 0.1 and 1 mM significantly (F2,23 = 15.21, P < 0.01) decreased endogenous growth of coleoptile segments by ∼50 %, as compared with growth in medium without Ca2+ and K+ (1554.4 ± 140.4 µm cm−1; mean ± SE, n = 8, Fig. 1A, inset). In the presence of 1 mM K+, endogenous growth of coleoptile segments was diminished only at 1 mM Ca2+ (F2,24 = 11.17, P < 0.01) (Fig. 1B). At 10 mM K+, endogenous growth of segments only slightly (especially over the first 6 h) depended on Ca2+ concentration (F2,24 = 3.47, P = 0.047) (Fig. 1C). Interestingly, in the absence of Ca2+ in the medium, elongation growth of coleoptile segments was comparable at all K+ concentrations studied (F2,25 = 1.25, P = 0.302) (Fig. 1).Figure 1.


Effect of K+ and Ca2+ on the indole-3-acetic acid- and fusicoccin-induced growth and membrane potential in maize coleoptile cells.

Siemieniuk A, Karcz W - AoB Plants (2015)

Effect of K+ and Ca2+ ions on the endogenous growth rate (growth without growth substances) of maize coleoptile segments. (A) Growth rate (µm s−1 cm−1) of coleoptile segments incubated in the medium with 0.0 mM K+ and Ca2+ at 0.0, 0.1 and 1.0 mM. (B) As in A but with 1.0 mM K+. (C) As in A and B but with 10 mM K+ in the medium. The growth rate of five coleoptile segments (10 mm in length) was recorded for 10 h in an intensively aerated incubation medium (5 mL segment−1) by means of an angular position transducer (TWK Electronic). Inset shows total elongation growth calculated as the sum of extensions measured in 3-min intervals for 10 h. All curves are mean values of at least eight independent measurements. Bars indicate ±SE.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4543891&req=5

PLV070F1: Effect of K+ and Ca2+ ions on the endogenous growth rate (growth without growth substances) of maize coleoptile segments. (A) Growth rate (µm s−1 cm−1) of coleoptile segments incubated in the medium with 0.0 mM K+ and Ca2+ at 0.0, 0.1 and 1.0 mM. (B) As in A but with 1.0 mM K+. (C) As in A and B but with 10 mM K+ in the medium. The growth rate of five coleoptile segments (10 mm in length) was recorded for 10 h in an intensively aerated incubation medium (5 mL segment−1) by means of an angular position transducer (TWK Electronic). Inset shows total elongation growth calculated as the sum of extensions measured in 3-min intervals for 10 h. All curves are mean values of at least eight independent measurements. Bars indicate ±SE.
Mentions: The effects of K+ (0.0, 1.0 and 10 mM) and Ca2+ (0.0, 0.1 and 1 mM) on the growth rate of maize coleoptile segments incubated in control medium (endogenous growth) are shown in Fig. 1. On the basis of growth rate responses, the total elongation growth (calculated as the sum of extensions from 3-min intervals) of coleoptile segments was also obtained (Fig. 1, insets). Taking these data into account, it should be stated that in media without K+, Ca2+ at 0.1 and 1 mM significantly (F2,23 = 15.21, P < 0.01) decreased endogenous growth of coleoptile segments by ∼50 %, as compared with growth in medium without Ca2+ and K+ (1554.4 ± 140.4 µm cm−1; mean ± SE, n = 8, Fig. 1A, inset). In the presence of 1 mM K+, endogenous growth of coleoptile segments was diminished only at 1 mM Ca2+ (F2,24 = 11.17, P < 0.01) (Fig. 1B). At 10 mM K+, endogenous growth of segments only slightly (especially over the first 6 h) depended on Ca2+ concentration (F2,24 = 3.47, P = 0.047) (Fig. 1C). Interestingly, in the absence of Ca2+ in the medium, elongation growth of coleoptile segments was comparable at all K+ concentrations studied (F2,25 = 1.25, P = 0.302) (Fig. 1).Figure 1.

Bottom Line: It was also found that in parenchymal cells of maize coleoptile segments, membrane potential (Em) was strongly affected by the medium K(+), independently of Ca(2+).However, lack of Ca(2+) in the incubation medium significantly reduced the IAA- and FC-induced membrane potential hyperpolarization.Verapamil did not change either the Em of parenchymal cells incubated in the control medium or the IAA- and FC-induced membrane hyperpolarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Physiology, Faculty of Biology and Environmental Protection, University of Silesia, Jagiellońska 28, 40-032 Katowice, Silesia, Poland.

No MeSH data available.


Related in: MedlinePlus