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Deciphering Asthma Biomarkers with Protein Profiling Technology.

Kuang Z, Wilson JJ, Luo S, Zhu SW, Huang RP - Int J Inflam (2015)

Bottom Line: Asthma is a chronic inflammatory disease of the airways, resulting in bronchial hyperresponsiveness with every allergen exposure.Protein biomarkers can serve as powerful detection tools in both clinical and basic research applications.Recent endeavors from biomedical researchers have developed technical platforms, such as cytokine antibody arrays, that have been employed and used to further the global analysis of asthma biomarker studies.

View Article: PubMed Central - PubMed

Affiliation: RayBiotech, Inc., Guangzhou 510600, China ; RayBiotech, Inc., 3607 Parkway Lane, Norcross, GA 30092, USA ; South China Biochip Research Center, Guangzhou 510600, China.

ABSTRACT
Asthma is a chronic inflammatory disease of the airways, resulting in bronchial hyperresponsiveness with every allergen exposure. It is now clear that asthma is not a single disease, but rather a multifaceted syndrome that results from a variety of biologic mechanisms. Asthma is further problematic given that the disease consists of many variants, each with its own etiologic and pathophysiologic factors, including different cellular responses and inflammatory phenotypes. These facets make the rapid and accurate diagnosis (not to mention treatments) of asthma extremely difficult. Protein biomarkers can serve as powerful detection tools in both clinical and basic research applications. Recent endeavors from biomedical researchers have developed technical platforms, such as cytokine antibody arrays, that have been employed and used to further the global analysis of asthma biomarker studies. In this review, we discuss potential asthma biomarkers involved in the pathophysiologic process and eventual pathogenesis of asthma, how these biomarkers are being utilized, and how further testing methods might help improve the diagnosis and treatment strain that current asthma patients suffer.

No MeSH data available.


Related in: MedlinePlus

The principle employed in most antibody arrays. (a) The sandwich method which requires an immobilized capture antibody and a labeled detection antibody. (b) Direct-label method with capture antibody and labeled analyte.
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fig1: The principle employed in most antibody arrays. (a) The sandwich method which requires an immobilized capture antibody and a labeled detection antibody. (b) Direct-label method with capture antibody and labeled analyte.

Mentions: The design principle of antibody arrays is usually based on either a sandwich based ELISA immunoassay or a direct-labeling of target proteins approach. Similar to common single target sandwich ELISA platforms, multiplex antibody array platforms utilize an antibody pair, where a capture antibody is immobilized on the surface of glass slides or nitrocellulose membranes and is paired with a labeled target specific detection antibody in solution (Figure 1(a)). This method has the advantages of excellent specificity and sensitivity given the dual binding requirements for target signal detection. The capture antibody is specific for one region of the target protein, while the detection antibody recognizes a different region of the target, combining to remove almost all potential for epitope cross-reactivity. However, the combinations and the number of protein targets to be measured in each array are limited because of cross-reactivity between detection antibodies within a single array. To overcome this restriction, multiple independent arrays with panels of nonoverlapping and cross-reactivity compatible antibody pairs can be employed to allow increasing numbers of cytokines to be measured [99].


Deciphering Asthma Biomarkers with Protein Profiling Technology.

Kuang Z, Wilson JJ, Luo S, Zhu SW, Huang RP - Int J Inflam (2015)

The principle employed in most antibody arrays. (a) The sandwich method which requires an immobilized capture antibody and a labeled detection antibody. (b) Direct-label method with capture antibody and labeled analyte.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4543788&req=5

fig1: The principle employed in most antibody arrays. (a) The sandwich method which requires an immobilized capture antibody and a labeled detection antibody. (b) Direct-label method with capture antibody and labeled analyte.
Mentions: The design principle of antibody arrays is usually based on either a sandwich based ELISA immunoassay or a direct-labeling of target proteins approach. Similar to common single target sandwich ELISA platforms, multiplex antibody array platforms utilize an antibody pair, where a capture antibody is immobilized on the surface of glass slides or nitrocellulose membranes and is paired with a labeled target specific detection antibody in solution (Figure 1(a)). This method has the advantages of excellent specificity and sensitivity given the dual binding requirements for target signal detection. The capture antibody is specific for one region of the target protein, while the detection antibody recognizes a different region of the target, combining to remove almost all potential for epitope cross-reactivity. However, the combinations and the number of protein targets to be measured in each array are limited because of cross-reactivity between detection antibodies within a single array. To overcome this restriction, multiple independent arrays with panels of nonoverlapping and cross-reactivity compatible antibody pairs can be employed to allow increasing numbers of cytokines to be measured [99].

Bottom Line: Asthma is a chronic inflammatory disease of the airways, resulting in bronchial hyperresponsiveness with every allergen exposure.Protein biomarkers can serve as powerful detection tools in both clinical and basic research applications.Recent endeavors from biomedical researchers have developed technical platforms, such as cytokine antibody arrays, that have been employed and used to further the global analysis of asthma biomarker studies.

View Article: PubMed Central - PubMed

Affiliation: RayBiotech, Inc., Guangzhou 510600, China ; RayBiotech, Inc., 3607 Parkway Lane, Norcross, GA 30092, USA ; South China Biochip Research Center, Guangzhou 510600, China.

ABSTRACT
Asthma is a chronic inflammatory disease of the airways, resulting in bronchial hyperresponsiveness with every allergen exposure. It is now clear that asthma is not a single disease, but rather a multifaceted syndrome that results from a variety of biologic mechanisms. Asthma is further problematic given that the disease consists of many variants, each with its own etiologic and pathophysiologic factors, including different cellular responses and inflammatory phenotypes. These facets make the rapid and accurate diagnosis (not to mention treatments) of asthma extremely difficult. Protein biomarkers can serve as powerful detection tools in both clinical and basic research applications. Recent endeavors from biomedical researchers have developed technical platforms, such as cytokine antibody arrays, that have been employed and used to further the global analysis of asthma biomarker studies. In this review, we discuss potential asthma biomarkers involved in the pathophysiologic process and eventual pathogenesis of asthma, how these biomarkers are being utilized, and how further testing methods might help improve the diagnosis and treatment strain that current asthma patients suffer.

No MeSH data available.


Related in: MedlinePlus