Limits...
Interleukin 37 Expression Inhibits STAT3 to Suppress the Proliferation and Invasion of Human Cervical Cancer Cells.

Wang S, An W, Yao Y, Chen R, Zheng X, Yang W, Zhao Y, Hu X, Jiang E, Bie Y, Chen Z, Ouyang P, Zhang H, Xiong H - J Cancer (2015)

Bottom Line: Firstly, we found that IL-37 inhibited STAT3 expression at both mRNA and protein levels.Secondly, blockage of STAT3 using siRNAs reduced significantly the ability of IL-37 to suppress cell proliferation and invasion.This study demonstrated a new biological function of IL-37 and offered a potential molecule for CC treatment.

View Article: PubMed Central - PubMed

Affiliation: 1. Cancer Institute, Guangdong Medical University, Dongguan 523808, China;

ABSTRACT

Objectives: The most recently discovered cytokine interleukin 37 (IL-37) received growing attention. Its function on tumor is largely unknown. Here, we investigated the biological function of IL-37 on cervical cancer (CC). Materials and methods : HPV(+) Hela cells and HPV(-) C33A cells were used. RT-qPCR was performed to detect the transcription of IL-37, STAT3, TNF-αand IL-1β. Western blotting was used for protein detection. CCK-8 assay and transwell assay were employed for cell proliferation and invasion detection, respectively. Results : Successful gene transfection of IL-37 suppressed the proliferation and invasion of CC. Interestingly, IL-37 showed higher anticancer ability in HPV(+) Hela cells than that in HPV(-) C33A cells. Then, the molecular mechanism of IL-37 anticancer was explored. Firstly, we found that IL-37 inhibited STAT3 expression at both mRNA and protein levels. IL-37 also down regulated the phosphorylation of STAT3. Secondly, blockage of STAT3 using siRNAs reduced significantly the ability of IL-37 to suppress cell proliferation and invasion. Thirdly, STAT3 knockdown reduced markedly the inhibition of IL-37 on the transcription of tumor-derived TNF-α and IL-1β, indicating the contribution of STAT3 for the cancer associated antiinflammation of IL-37. Finally, STAT3 up regulation restored the ability of cell proliferation, cell invasion and the expression of inflammatory cytokines, TNF-α and IL-1β. Conclusions : IL-37 suppressed cell proliferation and invasion of CC and STAT3 is involved in this process. Thus, IL-37 emerges as a new anticancer cytokine for CC. This study demonstrated a new biological function of IL-37 and offered a potential molecule for CC treatment.

No MeSH data available.


Related in: MedlinePlus

Expression of human IL-37 suppressed the proliferation and invasion of HPV+ Hela and HPV- C33A cells. (A, B) Transfection of IL-37 increased the mRNA expression of IL-37 in Hela (A) and C33A (B) cells. (C, D) Transfection of IL-37 increased the protein expression of IL-37 in Hela (C) and C33A (D) cells. (E, F) IL-37 inhibited the cell proliferation of Hela (E) and C33A (F) cells. (G, H) IL-37 suppressed the cell invasion of Hela (G) and C33A (H) cells. (I) IL-37 showed higher anticancer ability to suppress cell proliferation in HPV+ Hela cells than that in HPV- C33A cells. (J) IL-37 showed higher anticancer ability to suppress cell invasion in HPV+ Hela cells than that in HPV- C33A cells. (N=3). NC: normal control gourp (transfection of blank expression vector). IL-37: IL-37 gene transfection group.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4543756&req=5

Figure 1: Expression of human IL-37 suppressed the proliferation and invasion of HPV+ Hela and HPV- C33A cells. (A, B) Transfection of IL-37 increased the mRNA expression of IL-37 in Hela (A) and C33A (B) cells. (C, D) Transfection of IL-37 increased the protein expression of IL-37 in Hela (C) and C33A (D) cells. (E, F) IL-37 inhibited the cell proliferation of Hela (E) and C33A (F) cells. (G, H) IL-37 suppressed the cell invasion of Hela (G) and C33A (H) cells. (I) IL-37 showed higher anticancer ability to suppress cell proliferation in HPV+ Hela cells than that in HPV- C33A cells. (J) IL-37 showed higher anticancer ability to suppress cell invasion in HPV+ Hela cells than that in HPV- C33A cells. (N=3). NC: normal control gourp (transfection of blank expression vector). IL-37: IL-37 gene transfection group.

Mentions: IL-37 gene transfection induced successfully IL-37 expression in both Hela and C33A cells. IL-37 expression induced significant increase at IL-37 mRNA level, 700% and 2500% for Hela cells (Figure 1A) and C33A cells (Figure 1B), respectively. No clear protein bands were detected in normal cells by western blotting while thick electrophoretic bands of IL-37 proteins were found in cells of IL-37 transfection group (Figure 1C and Figure 1D). IL-37 gene expressed a 25KD protein in CC cells (Figure 1C and Figure 1D), which is similar to that in A549 cells and RAW cells 9.


Interleukin 37 Expression Inhibits STAT3 to Suppress the Proliferation and Invasion of Human Cervical Cancer Cells.

Wang S, An W, Yao Y, Chen R, Zheng X, Yang W, Zhao Y, Hu X, Jiang E, Bie Y, Chen Z, Ouyang P, Zhang H, Xiong H - J Cancer (2015)

Expression of human IL-37 suppressed the proliferation and invasion of HPV+ Hela and HPV- C33A cells. (A, B) Transfection of IL-37 increased the mRNA expression of IL-37 in Hela (A) and C33A (B) cells. (C, D) Transfection of IL-37 increased the protein expression of IL-37 in Hela (C) and C33A (D) cells. (E, F) IL-37 inhibited the cell proliferation of Hela (E) and C33A (F) cells. (G, H) IL-37 suppressed the cell invasion of Hela (G) and C33A (H) cells. (I) IL-37 showed higher anticancer ability to suppress cell proliferation in HPV+ Hela cells than that in HPV- C33A cells. (J) IL-37 showed higher anticancer ability to suppress cell invasion in HPV+ Hela cells than that in HPV- C33A cells. (N=3). NC: normal control gourp (transfection of blank expression vector). IL-37: IL-37 gene transfection group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4543756&req=5

Figure 1: Expression of human IL-37 suppressed the proliferation and invasion of HPV+ Hela and HPV- C33A cells. (A, B) Transfection of IL-37 increased the mRNA expression of IL-37 in Hela (A) and C33A (B) cells. (C, D) Transfection of IL-37 increased the protein expression of IL-37 in Hela (C) and C33A (D) cells. (E, F) IL-37 inhibited the cell proliferation of Hela (E) and C33A (F) cells. (G, H) IL-37 suppressed the cell invasion of Hela (G) and C33A (H) cells. (I) IL-37 showed higher anticancer ability to suppress cell proliferation in HPV+ Hela cells than that in HPV- C33A cells. (J) IL-37 showed higher anticancer ability to suppress cell invasion in HPV+ Hela cells than that in HPV- C33A cells. (N=3). NC: normal control gourp (transfection of blank expression vector). IL-37: IL-37 gene transfection group.
Mentions: IL-37 gene transfection induced successfully IL-37 expression in both Hela and C33A cells. IL-37 expression induced significant increase at IL-37 mRNA level, 700% and 2500% for Hela cells (Figure 1A) and C33A cells (Figure 1B), respectively. No clear protein bands were detected in normal cells by western blotting while thick electrophoretic bands of IL-37 proteins were found in cells of IL-37 transfection group (Figure 1C and Figure 1D). IL-37 gene expressed a 25KD protein in CC cells (Figure 1C and Figure 1D), which is similar to that in A549 cells and RAW cells 9.

Bottom Line: Firstly, we found that IL-37 inhibited STAT3 expression at both mRNA and protein levels.Secondly, blockage of STAT3 using siRNAs reduced significantly the ability of IL-37 to suppress cell proliferation and invasion.This study demonstrated a new biological function of IL-37 and offered a potential molecule for CC treatment.

View Article: PubMed Central - PubMed

Affiliation: 1. Cancer Institute, Guangdong Medical University, Dongguan 523808, China;

ABSTRACT

Objectives: The most recently discovered cytokine interleukin 37 (IL-37) received growing attention. Its function on tumor is largely unknown. Here, we investigated the biological function of IL-37 on cervical cancer (CC). Materials and methods : HPV(+) Hela cells and HPV(-) C33A cells were used. RT-qPCR was performed to detect the transcription of IL-37, STAT3, TNF-αand IL-1β. Western blotting was used for protein detection. CCK-8 assay and transwell assay were employed for cell proliferation and invasion detection, respectively. Results : Successful gene transfection of IL-37 suppressed the proliferation and invasion of CC. Interestingly, IL-37 showed higher anticancer ability in HPV(+) Hela cells than that in HPV(-) C33A cells. Then, the molecular mechanism of IL-37 anticancer was explored. Firstly, we found that IL-37 inhibited STAT3 expression at both mRNA and protein levels. IL-37 also down regulated the phosphorylation of STAT3. Secondly, blockage of STAT3 using siRNAs reduced significantly the ability of IL-37 to suppress cell proliferation and invasion. Thirdly, STAT3 knockdown reduced markedly the inhibition of IL-37 on the transcription of tumor-derived TNF-α and IL-1β, indicating the contribution of STAT3 for the cancer associated antiinflammation of IL-37. Finally, STAT3 up regulation restored the ability of cell proliferation, cell invasion and the expression of inflammatory cytokines, TNF-α and IL-1β. Conclusions : IL-37 suppressed cell proliferation and invasion of CC and STAT3 is involved in this process. Thus, IL-37 emerges as a new anticancer cytokine for CC. This study demonstrated a new biological function of IL-37 and offered a potential molecule for CC treatment.

No MeSH data available.


Related in: MedlinePlus