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Transcriptomic and Functional Pathway Analysis of Human Cervical Carcinoma Cancer Cells Response to Microtubule Inhibitor.

Wang J, Yan B, Liu SM, Sun H, Pan Y, Guan D, Zhang X, Xu J, Ma H - J Cancer (2015)

Bottom Line: We selected the concentration of vinblastine inducing 30% cell death for analyses assessing the DNA content, gene expression and transcriptional gene regulation of VBL-treated KB-3 cells.Transcriptomic and hierarchical clustering analysis demonstrated that treatment of KB-3 cells with VBL altered the expression of a diverse group of genes with G2/M arrest, which regulated by four oncogenic or tumor suppresser transcription factors (AP1, NFKB1, RELA, and TP53).Northern blots also confirmed that KRT-7, FN14, IER3, and ID1 were deregulated in VBL-treated KB-3 cells.

View Article: PubMed Central - PubMed

Affiliation: 1. Scientific Research Center, Shanghai Public Health Clinical Center, 2901 Caolang Road, Jinshan District, Shanghai 201508, China ; 2. Department of Translational Molecular Pathology, The University of Texas, M.D. Anderson Cancer Center, Houston, TX 77030, USA.

ABSTRACT

Background: There clearly is a need for effective chemotherapy for early-stage, high-risk patients with human cervical carcinoma. Vinblastine (VBL) is a key microtubule inhibitor, but unproven in its mechanisms as an important antitumor agent in cervical carcinoma.

Methods: We selected the concentration of vinblastine inducing 30% cell death for analyses assessing the DNA content, gene expression and transcriptional gene regulation of VBL-treated KB-3 cells.

Results: Transcriptomic and hierarchical clustering analysis demonstrated that treatment of KB-3 cells with VBL altered the expression of a diverse group of genes with G2/M arrest, which regulated by four oncogenic or tumor suppresser transcription factors (AP1, NFKB1, RELA, and TP53). Functional pathway analysis revealed the disease response to the biological effects of vinblastine in cervical carcinoma chemotherapy including protein ubiquitination pathway, RhoGDI signaling, integrin signaling, agranulocyte adhesion and biapedesis, and actin nucleation pathways. Northern blots also confirmed that KRT-7, FN14, IER3, and ID1 were deregulated in VBL-treated KB-3 cells.

Conclusion: Transcriptional time series profiles and a functional pathway analysis of VBL-treated KB-3 cells will provide a new strategy for improving microtubule inhibitor chemotherapy for cervical carcinoma.

No MeSH data available.


Related in: MedlinePlus

Northern Blots analysis of ID1, IER3, KRT-7 and FN14, and ß-actin was used to normalize the RNA quantity in each sample (A). Analyzed the quantification of the mRNA expression levels of ID1, IER3, KRT-7 and FN14 those normalized to ß-actin in KB-3 cells treated with vinblatine (B).
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Figure 3: Northern Blots analysis of ID1, IER3, KRT-7 and FN14, and ß-actin was used to normalize the RNA quantity in each sample (A). Analyzed the quantification of the mRNA expression levels of ID1, IER3, KRT-7 and FN14 those normalized to ß-actin in KB-3 cells treated with vinblatine (B).

Mentions: Northern blot analysis of ID1, IER3, KRT-7, FN14, and β-actin was performed on RNA prepared from KB-3 cells that were treated with 2.5 x 10-4 µM VBL for 0, 2, 4, 8, 12, 16, and 24 h and demonstrated that ID1, IER3, KRT-7, and FN14 were deregulated in KB-3 cells treated with VBL compared to β-actin as control (Fig. 3). Northern blot analysis of deregulated genes in KB-3 cells treated with VBL verified the array hybridization data.


Transcriptomic and Functional Pathway Analysis of Human Cervical Carcinoma Cancer Cells Response to Microtubule Inhibitor.

Wang J, Yan B, Liu SM, Sun H, Pan Y, Guan D, Zhang X, Xu J, Ma H - J Cancer (2015)

Northern Blots analysis of ID1, IER3, KRT-7 and FN14, and ß-actin was used to normalize the RNA quantity in each sample (A). Analyzed the quantification of the mRNA expression levels of ID1, IER3, KRT-7 and FN14 those normalized to ß-actin in KB-3 cells treated with vinblatine (B).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4543753&req=5

Figure 3: Northern Blots analysis of ID1, IER3, KRT-7 and FN14, and ß-actin was used to normalize the RNA quantity in each sample (A). Analyzed the quantification of the mRNA expression levels of ID1, IER3, KRT-7 and FN14 those normalized to ß-actin in KB-3 cells treated with vinblatine (B).
Mentions: Northern blot analysis of ID1, IER3, KRT-7, FN14, and β-actin was performed on RNA prepared from KB-3 cells that were treated with 2.5 x 10-4 µM VBL for 0, 2, 4, 8, 12, 16, and 24 h and demonstrated that ID1, IER3, KRT-7, and FN14 were deregulated in KB-3 cells treated with VBL compared to β-actin as control (Fig. 3). Northern blot analysis of deregulated genes in KB-3 cells treated with VBL verified the array hybridization data.

Bottom Line: We selected the concentration of vinblastine inducing 30% cell death for analyses assessing the DNA content, gene expression and transcriptional gene regulation of VBL-treated KB-3 cells.Transcriptomic and hierarchical clustering analysis demonstrated that treatment of KB-3 cells with VBL altered the expression of a diverse group of genes with G2/M arrest, which regulated by four oncogenic or tumor suppresser transcription factors (AP1, NFKB1, RELA, and TP53).Northern blots also confirmed that KRT-7, FN14, IER3, and ID1 were deregulated in VBL-treated KB-3 cells.

View Article: PubMed Central - PubMed

Affiliation: 1. Scientific Research Center, Shanghai Public Health Clinical Center, 2901 Caolang Road, Jinshan District, Shanghai 201508, China ; 2. Department of Translational Molecular Pathology, The University of Texas, M.D. Anderson Cancer Center, Houston, TX 77030, USA.

ABSTRACT

Background: There clearly is a need for effective chemotherapy for early-stage, high-risk patients with human cervical carcinoma. Vinblastine (VBL) is a key microtubule inhibitor, but unproven in its mechanisms as an important antitumor agent in cervical carcinoma.

Methods: We selected the concentration of vinblastine inducing 30% cell death for analyses assessing the DNA content, gene expression and transcriptional gene regulation of VBL-treated KB-3 cells.

Results: Transcriptomic and hierarchical clustering analysis demonstrated that treatment of KB-3 cells with VBL altered the expression of a diverse group of genes with G2/M arrest, which regulated by four oncogenic or tumor suppresser transcription factors (AP1, NFKB1, RELA, and TP53). Functional pathway analysis revealed the disease response to the biological effects of vinblastine in cervical carcinoma chemotherapy including protein ubiquitination pathway, RhoGDI signaling, integrin signaling, agranulocyte adhesion and biapedesis, and actin nucleation pathways. Northern blots also confirmed that KRT-7, FN14, IER3, and ID1 were deregulated in VBL-treated KB-3 cells.

Conclusion: Transcriptional time series profiles and a functional pathway analysis of VBL-treated KB-3 cells will provide a new strategy for improving microtubule inhibitor chemotherapy for cervical carcinoma.

No MeSH data available.


Related in: MedlinePlus