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Gene expression changes in subcutaneous adipose tissue due to Cushing's disease.

Hochberg I, Harvey I, Tran QT, Stephenson EJ, Barkan AL, Saltiel AR, Chandler WF, Bridges D - J. Mol. Endocrinol. (2015)

Bottom Line: Glucocorticoids have major effects on adipose tissue metabolism.We found a higher expression of transcripts involved in several metabolic pathways, including lipogenesis, proteolysis and glucose oxidation as well as a decreased expression of transcripts involved in inflammation and protein synthesis.These data provide insight to transcriptional changes that may be responsible for the comorbidities associated with chronic elevations of glucocorticoids.

View Article: PubMed Central - PubMed

Affiliation: Institute of EndocrinologyDiabetes and Metabolism, Rambam Health Care Campus, Haifa, IsraelLife Science InstituteUniversity of Michigan, Ann Arbor, MI, USAPhysiologyUTHSC, Memphis, TN, USAPreventive MedicineUTHSC, Memphis, TN, USAInternal MedicineUniversity of Michigan, Ann Arbor, MI USANeurosurgeryUniversity of Michigan, Ann Arbor, MI USAPediatricsUTHSC, Memphis, TN, USA Institute of EndocrinologyDiabetes and Metabolism, Rambam Health Care Campus, Haifa, IsraelLife Science InstituteUniversity of Michigan, Ann Arbor, MI, USAPhysiologyUTHSC, Memphis, TN, USAPreventive MedicineUTHSC, Memphis, TN, USAInternal MedicineUniversity of Michigan, Ann Arbor, MI USANeurosurgeryUniversity of Michigan, Ann Arbor, MI USAPediatricsUTHSC, Memphis, TN, USA i_hochberg@rambam.health.gov.il dbridge9@uthsc.edu.

No MeSH data available.


Related in: MedlinePlus

Glycolysis and glucose oxidation genes are upregulated with elevated glucocorticoids. Schematic of (A) glycolysis and (B) the TCA cycle, colored by gene expression changes in subcutaneous adipose tissue from Cushing's disease subjects. (C) qPCR analysis of selected glucose oxidation genes from mouse subcutaneous adipose tissue after 12 weeks of dexamethasone treatment. *q<0.05.
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fig5: Glycolysis and glucose oxidation genes are upregulated with elevated glucocorticoids. Schematic of (A) glycolysis and (B) the TCA cycle, colored by gene expression changes in subcutaneous adipose tissue from Cushing's disease subjects. (C) qPCR analysis of selected glucose oxidation genes from mouse subcutaneous adipose tissue after 12 weeks of dexamethasone treatment. *q<0.05.

Mentions: Several glucose metabolism genes, and specifically glycolysis and tricyclic acid (TCA) cycle genes were expressed at higher levels in Cushing's disease patients (Fig. 5). Strongly induced genes included HK3, FBP1, ALDOC, ENO1, IDH1, ME1 and DLAT. Upregulations in IDH1 and ME1 were also noted in the mouse adipose tissue, along with other transcripts involved in glucose oxidation such as ACO1, LDHB and MDH1 (Fig. 5C).


Gene expression changes in subcutaneous adipose tissue due to Cushing's disease.

Hochberg I, Harvey I, Tran QT, Stephenson EJ, Barkan AL, Saltiel AR, Chandler WF, Bridges D - J. Mol. Endocrinol. (2015)

Glycolysis and glucose oxidation genes are upregulated with elevated glucocorticoids. Schematic of (A) glycolysis and (B) the TCA cycle, colored by gene expression changes in subcutaneous adipose tissue from Cushing's disease subjects. (C) qPCR analysis of selected glucose oxidation genes from mouse subcutaneous adipose tissue after 12 weeks of dexamethasone treatment. *q<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4543687&req=5

fig5: Glycolysis and glucose oxidation genes are upregulated with elevated glucocorticoids. Schematic of (A) glycolysis and (B) the TCA cycle, colored by gene expression changes in subcutaneous adipose tissue from Cushing's disease subjects. (C) qPCR analysis of selected glucose oxidation genes from mouse subcutaneous adipose tissue after 12 weeks of dexamethasone treatment. *q<0.05.
Mentions: Several glucose metabolism genes, and specifically glycolysis and tricyclic acid (TCA) cycle genes were expressed at higher levels in Cushing's disease patients (Fig. 5). Strongly induced genes included HK3, FBP1, ALDOC, ENO1, IDH1, ME1 and DLAT. Upregulations in IDH1 and ME1 were also noted in the mouse adipose tissue, along with other transcripts involved in glucose oxidation such as ACO1, LDHB and MDH1 (Fig. 5C).

Bottom Line: Glucocorticoids have major effects on adipose tissue metabolism.We found a higher expression of transcripts involved in several metabolic pathways, including lipogenesis, proteolysis and glucose oxidation as well as a decreased expression of transcripts involved in inflammation and protein synthesis.These data provide insight to transcriptional changes that may be responsible for the comorbidities associated with chronic elevations of glucocorticoids.

View Article: PubMed Central - PubMed

Affiliation: Institute of EndocrinologyDiabetes and Metabolism, Rambam Health Care Campus, Haifa, IsraelLife Science InstituteUniversity of Michigan, Ann Arbor, MI, USAPhysiologyUTHSC, Memphis, TN, USAPreventive MedicineUTHSC, Memphis, TN, USAInternal MedicineUniversity of Michigan, Ann Arbor, MI USANeurosurgeryUniversity of Michigan, Ann Arbor, MI USAPediatricsUTHSC, Memphis, TN, USA Institute of EndocrinologyDiabetes and Metabolism, Rambam Health Care Campus, Haifa, IsraelLife Science InstituteUniversity of Michigan, Ann Arbor, MI, USAPhysiologyUTHSC, Memphis, TN, USAPreventive MedicineUTHSC, Memphis, TN, USAInternal MedicineUniversity of Michigan, Ann Arbor, MI USANeurosurgeryUniversity of Michigan, Ann Arbor, MI USAPediatricsUTHSC, Memphis, TN, USA i_hochberg@rambam.health.gov.il dbridge9@uthsc.edu.

No MeSH data available.


Related in: MedlinePlus