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Gene expression changes in subcutaneous adipose tissue due to Cushing's disease.

Hochberg I, Harvey I, Tran QT, Stephenson EJ, Barkan AL, Saltiel AR, Chandler WF, Bridges D - J. Mol. Endocrinol. (2015)

Bottom Line: Glucocorticoids have major effects on adipose tissue metabolism.We found a higher expression of transcripts involved in several metabolic pathways, including lipogenesis, proteolysis and glucose oxidation as well as a decreased expression of transcripts involved in inflammation and protein synthesis.These data provide insight to transcriptional changes that may be responsible for the comorbidities associated with chronic elevations of glucocorticoids.

View Article: PubMed Central - PubMed

Affiliation: Institute of EndocrinologyDiabetes and Metabolism, Rambam Health Care Campus, Haifa, IsraelLife Science InstituteUniversity of Michigan, Ann Arbor, MI, USAPhysiologyUTHSC, Memphis, TN, USAPreventive MedicineUTHSC, Memphis, TN, USAInternal MedicineUniversity of Michigan, Ann Arbor, MI USANeurosurgeryUniversity of Michigan, Ann Arbor, MI USAPediatricsUTHSC, Memphis, TN, USA Institute of EndocrinologyDiabetes and Metabolism, Rambam Health Care Campus, Haifa, IsraelLife Science InstituteUniversity of Michigan, Ann Arbor, MI, USAPhysiologyUTHSC, Memphis, TN, USAPreventive MedicineUTHSC, Memphis, TN, USAInternal MedicineUniversity of Michigan, Ann Arbor, MI USANeurosurgeryUniversity of Michigan, Ann Arbor, MI USAPediatricsUTHSC, Memphis, TN, USA i_hochberg@rambam.health.gov.il dbridge9@uthsc.edu.

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Related in: MedlinePlus

Elevated glucocorticoids result in elevated fatty acid and triglyceride synthesis genes. (A) Fatty acid synthesis genes in Cushing's disease and control patients. (B) Fatty acid desaturases in Cushing's disease patients. (C) Triglyceride synthesis genes. (D) Lipolysis genes. (E) Steroid biogenesis genes. (F) Evaluation of lipogenic genes in mouse subcutaneous adipose tissue. *q<0.05.
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fig4: Elevated glucocorticoids result in elevated fatty acid and triglyceride synthesis genes. (A) Fatty acid synthesis genes in Cushing's disease and control patients. (B) Fatty acid desaturases in Cushing's disease patients. (C) Triglyceride synthesis genes. (D) Lipolysis genes. (E) Steroid biogenesis genes. (F) Evaluation of lipogenic genes in mouse subcutaneous adipose tissue. *q<0.05.

Mentions: Increased subcutaneous fat mass is a hallmark of Cushing's syndrome and could potentially be mediated through the activation of adipogenesis or lipogenesis. Our transcriptomic data support the hypothesis that lipogenesis is activated in these tissues via the transcriptional activation of fatty acid synthesis and triglyceride synthesis. All of the major genes involved in the synthesis of fatty acids were expressed at higher levels including ACACA, FASN, ACSL1/3/4 and ELOVL1/5/6 (Fig. 4A). Desaturation of fatty acids is an essential aspect of de novo fatty acid synthesis, and we also observed elevations in all fatty acid desaturases SCD, FADS1, FADS2 and HSD17B12 (Fig. 4B). The triglyceride synthesis genes GPAM, DGAT1, DGAT2, AGPAT2/3 and GPD1 were also upregulated in the subcutaneous adipose tissue from Cushing's disease patients (Fig. 4C).


Gene expression changes in subcutaneous adipose tissue due to Cushing's disease.

Hochberg I, Harvey I, Tran QT, Stephenson EJ, Barkan AL, Saltiel AR, Chandler WF, Bridges D - J. Mol. Endocrinol. (2015)

Elevated glucocorticoids result in elevated fatty acid and triglyceride synthesis genes. (A) Fatty acid synthesis genes in Cushing's disease and control patients. (B) Fatty acid desaturases in Cushing's disease patients. (C) Triglyceride synthesis genes. (D) Lipolysis genes. (E) Steroid biogenesis genes. (F) Evaluation of lipogenic genes in mouse subcutaneous adipose tissue. *q<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4543687&req=5

fig4: Elevated glucocorticoids result in elevated fatty acid and triglyceride synthesis genes. (A) Fatty acid synthesis genes in Cushing's disease and control patients. (B) Fatty acid desaturases in Cushing's disease patients. (C) Triglyceride synthesis genes. (D) Lipolysis genes. (E) Steroid biogenesis genes. (F) Evaluation of lipogenic genes in mouse subcutaneous adipose tissue. *q<0.05.
Mentions: Increased subcutaneous fat mass is a hallmark of Cushing's syndrome and could potentially be mediated through the activation of adipogenesis or lipogenesis. Our transcriptomic data support the hypothesis that lipogenesis is activated in these tissues via the transcriptional activation of fatty acid synthesis and triglyceride synthesis. All of the major genes involved in the synthesis of fatty acids were expressed at higher levels including ACACA, FASN, ACSL1/3/4 and ELOVL1/5/6 (Fig. 4A). Desaturation of fatty acids is an essential aspect of de novo fatty acid synthesis, and we also observed elevations in all fatty acid desaturases SCD, FADS1, FADS2 and HSD17B12 (Fig. 4B). The triglyceride synthesis genes GPAM, DGAT1, DGAT2, AGPAT2/3 and GPD1 were also upregulated in the subcutaneous adipose tissue from Cushing's disease patients (Fig. 4C).

Bottom Line: Glucocorticoids have major effects on adipose tissue metabolism.We found a higher expression of transcripts involved in several metabolic pathways, including lipogenesis, proteolysis and glucose oxidation as well as a decreased expression of transcripts involved in inflammation and protein synthesis.These data provide insight to transcriptional changes that may be responsible for the comorbidities associated with chronic elevations of glucocorticoids.

View Article: PubMed Central - PubMed

Affiliation: Institute of EndocrinologyDiabetes and Metabolism, Rambam Health Care Campus, Haifa, IsraelLife Science InstituteUniversity of Michigan, Ann Arbor, MI, USAPhysiologyUTHSC, Memphis, TN, USAPreventive MedicineUTHSC, Memphis, TN, USAInternal MedicineUniversity of Michigan, Ann Arbor, MI USANeurosurgeryUniversity of Michigan, Ann Arbor, MI USAPediatricsUTHSC, Memphis, TN, USA Institute of EndocrinologyDiabetes and Metabolism, Rambam Health Care Campus, Haifa, IsraelLife Science InstituteUniversity of Michigan, Ann Arbor, MI, USAPhysiologyUTHSC, Memphis, TN, USAPreventive MedicineUTHSC, Memphis, TN, USAInternal MedicineUniversity of Michigan, Ann Arbor, MI USANeurosurgeryUniversity of Michigan, Ann Arbor, MI USAPediatricsUTHSC, Memphis, TN, USA i_hochberg@rambam.health.gov.il dbridge9@uthsc.edu.

No MeSH data available.


Related in: MedlinePlus