Limits...
A brave new MYC-amplified world.

Weinberg MS, Hart JR, Vogt PK - Aging (Albany NY) (2015)

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA.

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

MYC is a basic domain helix-loop-helix leucine zipper (bHLH-LZ) transcriptional regulator that forms a heterodimer with the related but much smaller bHLH-LZ protein Max to bind sequence-specifically to DNA... However, recent papers now extend MYC's broad activity to the entire non-coding transcriptome, showing that all non-coding RNAs are subject to MYC regulation... These data are in accord with the role of MYC as general transcriptional amplifier... A larger and far less explored segment of the non-coding transcriptome is made up of long non-coding RNAs (lncRNAs) which typically form functional secondary and higher order structures comprising protein-protein or protein-nucleic acid complexes... Considering that the vast majority of all transcribed sequences is non-coding, their inclusion among the transcriptional clients of MYC amounts to multiplying the MYC universe... The effect of MYC on non-coding transcripts is correlated with MYC binding to promoter-proximal sites and therefore appears to be a direct function of MYC, not mediated by a different downstream transcriptional regulator... As is the case with the coding transcriptome, MYC can function as a positive as well as negative regulator of transcription... The genome-wide effects of MYC on the non-coding transcriptome were discovered by RNAseq analysis... However, for selected genes, these data have been independently validated for specific lncRNAs by determining steady-state RNA levels by qRT-PCR and direct transcriptional effects by nuclear run-on experiments... MYC is also arguably the "Emperor of Oncogenes", involved as a driving force in most and probably all human cancers... A complete understanding of the mechanisms by which MYC achieves these oncogenic effects remains a daunting challenge, a challenge that has been considerably magnified by the regulatory role of MYC in the noncoding transcriptome... Exciting new genome editing and modulatory technologies will likely be adopted to generate a first compendium of cancer-relevant lncRNAs, while providing hints of their underlying function in the cell... It is a task that promises to provide critical new insights into the molecular biology of cancer.

No MeSH data available.


Related in: MedlinePlus

MYC regulates the expression of noncoding (nc) RNAsA heatmap of ncRNAs detected in the human B cell line P493–6. P493–6 cells were analyzed by RNAseq in triplicate under conditions of high and low MYC expression. Transcripts are ordered by the change in expression upon MYC upregulation, with those most upregulated at the top and those most downregulated at the bottom. The figure shows that the expression of virtually all ncRNAs is affected by MYC; they are either upregulated or downregulated. There extremely few if any noncoding transcripts that do not respond to MYC.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4543031&req=5

Figure 1: MYC regulates the expression of noncoding (nc) RNAsA heatmap of ncRNAs detected in the human B cell line P493–6. P493–6 cells were analyzed by RNAseq in triplicate under conditions of high and low MYC expression. Transcripts are ordered by the change in expression upon MYC upregulation, with those most upregulated at the top and those most downregulated at the bottom. The figure shows that the expression of virtually all ncRNAs is affected by MYC; they are either upregulated or downregulated. There extremely few if any noncoding transcripts that do not respond to MYC.


A brave new MYC-amplified world.

Weinberg MS, Hart JR, Vogt PK - Aging (Albany NY) (2015)

MYC regulates the expression of noncoding (nc) RNAsA heatmap of ncRNAs detected in the human B cell line P493–6. P493–6 cells were analyzed by RNAseq in triplicate under conditions of high and low MYC expression. Transcripts are ordered by the change in expression upon MYC upregulation, with those most upregulated at the top and those most downregulated at the bottom. The figure shows that the expression of virtually all ncRNAs is affected by MYC; they are either upregulated or downregulated. There extremely few if any noncoding transcripts that do not respond to MYC.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4543031&req=5

Figure 1: MYC regulates the expression of noncoding (nc) RNAsA heatmap of ncRNAs detected in the human B cell line P493–6. P493–6 cells were analyzed by RNAseq in triplicate under conditions of high and low MYC expression. Transcripts are ordered by the change in expression upon MYC upregulation, with those most upregulated at the top and those most downregulated at the bottom. The figure shows that the expression of virtually all ncRNAs is affected by MYC; they are either upregulated or downregulated. There extremely few if any noncoding transcripts that do not respond to MYC.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA.

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

MYC is a basic domain helix-loop-helix leucine zipper (bHLH-LZ) transcriptional regulator that forms a heterodimer with the related but much smaller bHLH-LZ protein Max to bind sequence-specifically to DNA... However, recent papers now extend MYC's broad activity to the entire non-coding transcriptome, showing that all non-coding RNAs are subject to MYC regulation... These data are in accord with the role of MYC as general transcriptional amplifier... A larger and far less explored segment of the non-coding transcriptome is made up of long non-coding RNAs (lncRNAs) which typically form functional secondary and higher order structures comprising protein-protein or protein-nucleic acid complexes... Considering that the vast majority of all transcribed sequences is non-coding, their inclusion among the transcriptional clients of MYC amounts to multiplying the MYC universe... The effect of MYC on non-coding transcripts is correlated with MYC binding to promoter-proximal sites and therefore appears to be a direct function of MYC, not mediated by a different downstream transcriptional regulator... As is the case with the coding transcriptome, MYC can function as a positive as well as negative regulator of transcription... The genome-wide effects of MYC on the non-coding transcriptome were discovered by RNAseq analysis... However, for selected genes, these data have been independently validated for specific lncRNAs by determining steady-state RNA levels by qRT-PCR and direct transcriptional effects by nuclear run-on experiments... MYC is also arguably the "Emperor of Oncogenes", involved as a driving force in most and probably all human cancers... A complete understanding of the mechanisms by which MYC achieves these oncogenic effects remains a daunting challenge, a challenge that has been considerably magnified by the regulatory role of MYC in the noncoding transcriptome... Exciting new genome editing and modulatory technologies will likely be adopted to generate a first compendium of cancer-relevant lncRNAs, while providing hints of their underlying function in the cell... It is a task that promises to provide critical new insights into the molecular biology of cancer.

No MeSH data available.


Related in: MedlinePlus