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Distorted genetic segregation of the transposon mPing at the long arm of chromosome 12 in rice.

Horibata A, Kakikubo Y, Kato T - Breed. Sci. (2015)

Bottom Line: These results, however, could not explain the lower transmission of male gametes with mPing.At least two ORFs, whose functions have not been identified, are located near this mPing.It is plausible that either of these ORFs or both are necessary for the normal functioning of male gametes.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology-Oriented Science and Technology, Kinki University , 930 Nishimitani, Kinokawa, Wakayama 649-6493 , Japan.

ABSTRACT
A class II transposable element, mPing exists in the rice genome ubiquitously and can transpose even in ordinary cultivation conditions. A copy of mPing was identified at the long arm of chromosome 12. In reciprocal backcrossed F1s between a heterozygote and a homozygote without mPing, the male gametes with this mPing from heterozygotes were transmitted to the next generation at a lower frequency than those without mPing, resulting in distorted genetic segregation in self-fertilized progenies, as well as in F1s after backcrossing. Pollens with mPing tended to germinate on stigma less than those without mPing. These results, however, could not explain the lower transmission of male gametes with mPing. In addition, no excision of mPing was observed in a homozygote. Thus, it was suggested that male gametes with mPing were eliminated partly from pollination to fertilization by negative competition against male gametes without mPing. Less formation of microspores with mPing in meiosis could also be a cause for the distorted segregation, although this could not be examined. At least two ORFs, whose functions have not been identified, are located near this mPing. It is plausible that either of these ORFs or both are necessary for the normal functioning of male gametes.

No MeSH data available.


Related in: MedlinePlus

Relationships between the positions of inserted mPing and two ORFs near the long arm of rice chromosome 12. This figure is based on Rice Annotation Project-Database (RAP-DB), IRGSP build 5. nt, nucleotide.
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f2-65_340: Relationships between the positions of inserted mPing and two ORFs near the long arm of rice chromosome 12. This figure is based on Rice Annotation Project-Database (RAP-DB), IRGSP build 5. nt, nucleotide.

Mentions: According to the RAP-DB (IRGSP build 5), there are at least two putative ORFs near the inserted mPing: Os12g0588400 (24,595,783..24,596,141) and Os12g0588200 (24,589,162..249,591,131), which are located on both sides of mPing (Fig. 2). So far, the functions of these two ORFs have not been identified from current database searches. Expression analysis should be conducted for these two ORFs. However, this should be done individually in male gametes with and without mPing produced from heterozygotes. Although the ORFs located near the insertion site of mPing at the long arm of chromosome 12 have not been identified as genes concerned with male gamete functions, the present results demonstrate that mPing can be utilized effectively as a tool of gene tagging in rice.


Distorted genetic segregation of the transposon mPing at the long arm of chromosome 12 in rice.

Horibata A, Kakikubo Y, Kato T - Breed. Sci. (2015)

Relationships between the positions of inserted mPing and two ORFs near the long arm of rice chromosome 12. This figure is based on Rice Annotation Project-Database (RAP-DB), IRGSP build 5. nt, nucleotide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542935&req=5

f2-65_340: Relationships between the positions of inserted mPing and two ORFs near the long arm of rice chromosome 12. This figure is based on Rice Annotation Project-Database (RAP-DB), IRGSP build 5. nt, nucleotide.
Mentions: According to the RAP-DB (IRGSP build 5), there are at least two putative ORFs near the inserted mPing: Os12g0588400 (24,595,783..24,596,141) and Os12g0588200 (24,589,162..249,591,131), which are located on both sides of mPing (Fig. 2). So far, the functions of these two ORFs have not been identified from current database searches. Expression analysis should be conducted for these two ORFs. However, this should be done individually in male gametes with and without mPing produced from heterozygotes. Although the ORFs located near the insertion site of mPing at the long arm of chromosome 12 have not been identified as genes concerned with male gamete functions, the present results demonstrate that mPing can be utilized effectively as a tool of gene tagging in rice.

Bottom Line: These results, however, could not explain the lower transmission of male gametes with mPing.At least two ORFs, whose functions have not been identified, are located near this mPing.It is plausible that either of these ORFs or both are necessary for the normal functioning of male gametes.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology-Oriented Science and Technology, Kinki University , 930 Nishimitani, Kinokawa, Wakayama 649-6493 , Japan.

ABSTRACT
A class II transposable element, mPing exists in the rice genome ubiquitously and can transpose even in ordinary cultivation conditions. A copy of mPing was identified at the long arm of chromosome 12. In reciprocal backcrossed F1s between a heterozygote and a homozygote without mPing, the male gametes with this mPing from heterozygotes were transmitted to the next generation at a lower frequency than those without mPing, resulting in distorted genetic segregation in self-fertilized progenies, as well as in F1s after backcrossing. Pollens with mPing tended to germinate on stigma less than those without mPing. These results, however, could not explain the lower transmission of male gametes with mPing. In addition, no excision of mPing was observed in a homozygote. Thus, it was suggested that male gametes with mPing were eliminated partly from pollination to fertilization by negative competition against male gametes without mPing. Less formation of microspores with mPing in meiosis could also be a cause for the distorted segregation, although this could not be examined. At least two ORFs, whose functions have not been identified, are located near this mPing. It is plausible that either of these ORFs or both are necessary for the normal functioning of male gametes.

No MeSH data available.


Related in: MedlinePlus