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Development of photoperiod- and thermo-sensitive male sterility rice expressing transgene Bacillus thuringiensis.

Liu X, Zhang J, Zhang C, Wang L, Chen H, Zhu Z, Tu J - Breed. Sci. (2015)

Bottom Line: An analysis of fertility transition traits indicated that 208S was completely sterile under long day length/high temperature, but partially fertile under short day length/low temperature.With fine grain quality and favorable combining ability, 208S had no observed negative effects on fertility and agronomic traits from Bt (cry1Ab/1Ac).Additionally, 208S as a male sterile line showed no fertility decrease caused by Bt transgenic process, as it is the case in Huahui 1.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University , Yuhangtang Road 866, Hangzhou, 310058 , China.

ABSTRACT
Stem borers and leaffolders are the main pests that cause severe damage in rice (Oryza sativa L.) production worldwide. We developed the first photoperiod- and thermo-sensitive male sterility (PTSMS) rice 208S with the cry1Ab/1Ac Bacillus thuringiensis (Bt) gene, through sexual crossing with Huahui 1 (elite line with the cry1Ab/1Ac gene). The novel 208S and its hybrids presented high and stable resistance to stem borers and leaffolders, and the content of Cry1Ab/1Ac protein in chlorophyllous tissues achieved the identical level as donor and showed little accumulation in non-chlorophyllous tissue. No dominant dosage effect in the Bt gene was observed in 208S and its derived hybrids. An analysis of fertility transition traits indicated that 208S was completely sterile under long day length/high temperature, but partially fertile under short day length/low temperature. With fine grain quality and favorable combining ability, 208S had no observed negative effects on fertility and agronomic traits from Bt (cry1Ab/1Ac). Additionally, 208S as a male sterile line showed no fertility decrease caused by Bt transgenic process, as it is the case in Huahui 1. Thus, 208S has great application value in two-line hybrid production for insect resistance, and can also be used as a bridge material in rice Bt transgenic breeding.

No MeSH data available.


Related in: MedlinePlus

Cry1Ab/1Ac concentration detected in donor line Huahui 1, 208S and its hybrid Zheyou 3 at tillering, heading and filling stages. (A–D) Cry1Ab/1Ac concentration detected in the leaf, stem, root and endosperm, respectively. Error bars indicate ± SD (n = 3). The x-axis represents corresponding rice lines containing the Bt gene.
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f3-65_333: Cry1Ab/1Ac concentration detected in donor line Huahui 1, 208S and its hybrid Zheyou 3 at tillering, heading and filling stages. (A–D) Cry1Ab/1Ac concentration detected in the leaf, stem, root and endosperm, respectively. Error bars indicate ± SD (n = 3). The x-axis represents corresponding rice lines containing the Bt gene.

Mentions: Quantitative analysis of Cry1Ab/1Ac protein showed that the Bt protein contents in 208S and its hybrid Zheyou 3 decreased with plant growth. This was particularly obvious in leaves where Bt protein content fell from 13.6 μg g−1 at tillering stage to 6.1 μg g−1 at grain filling stage, respectively, in 208S. A similar trend was seen in its hybrid progeny. Bt protein contents varied considerably between different tissues (Fig. 3); concentration of Cry1Ab/1Ac protein was obviously higher in the leaf and stem than in the endosperm and root. The Cry1Ab/1Ac protein contents in 208S and its hybrid were identical to the levels in donor parent Huahui 1, and there was no significant difference between the two at each growth stage of different tissues (Fig. 3). These results indicated that dosage effect of the gene had little effect on the concentration of Bt protein.


Development of photoperiod- and thermo-sensitive male sterility rice expressing transgene Bacillus thuringiensis.

Liu X, Zhang J, Zhang C, Wang L, Chen H, Zhu Z, Tu J - Breed. Sci. (2015)

Cry1Ab/1Ac concentration detected in donor line Huahui 1, 208S and its hybrid Zheyou 3 at tillering, heading and filling stages. (A–D) Cry1Ab/1Ac concentration detected in the leaf, stem, root and endosperm, respectively. Error bars indicate ± SD (n = 3). The x-axis represents corresponding rice lines containing the Bt gene.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542934&req=5

f3-65_333: Cry1Ab/1Ac concentration detected in donor line Huahui 1, 208S and its hybrid Zheyou 3 at tillering, heading and filling stages. (A–D) Cry1Ab/1Ac concentration detected in the leaf, stem, root and endosperm, respectively. Error bars indicate ± SD (n = 3). The x-axis represents corresponding rice lines containing the Bt gene.
Mentions: Quantitative analysis of Cry1Ab/1Ac protein showed that the Bt protein contents in 208S and its hybrid Zheyou 3 decreased with plant growth. This was particularly obvious in leaves where Bt protein content fell from 13.6 μg g−1 at tillering stage to 6.1 μg g−1 at grain filling stage, respectively, in 208S. A similar trend was seen in its hybrid progeny. Bt protein contents varied considerably between different tissues (Fig. 3); concentration of Cry1Ab/1Ac protein was obviously higher in the leaf and stem than in the endosperm and root. The Cry1Ab/1Ac protein contents in 208S and its hybrid were identical to the levels in donor parent Huahui 1, and there was no significant difference between the two at each growth stage of different tissues (Fig. 3). These results indicated that dosage effect of the gene had little effect on the concentration of Bt protein.

Bottom Line: An analysis of fertility transition traits indicated that 208S was completely sterile under long day length/high temperature, but partially fertile under short day length/low temperature.With fine grain quality and favorable combining ability, 208S had no observed negative effects on fertility and agronomic traits from Bt (cry1Ab/1Ac).Additionally, 208S as a male sterile line showed no fertility decrease caused by Bt transgenic process, as it is the case in Huahui 1.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University , Yuhangtang Road 866, Hangzhou, 310058 , China.

ABSTRACT
Stem borers and leaffolders are the main pests that cause severe damage in rice (Oryza sativa L.) production worldwide. We developed the first photoperiod- and thermo-sensitive male sterility (PTSMS) rice 208S with the cry1Ab/1Ac Bacillus thuringiensis (Bt) gene, through sexual crossing with Huahui 1 (elite line with the cry1Ab/1Ac gene). The novel 208S and its hybrids presented high and stable resistance to stem borers and leaffolders, and the content of Cry1Ab/1Ac protein in chlorophyllous tissues achieved the identical level as donor and showed little accumulation in non-chlorophyllous tissue. No dominant dosage effect in the Bt gene was observed in 208S and its derived hybrids. An analysis of fertility transition traits indicated that 208S was completely sterile under long day length/high temperature, but partially fertile under short day length/low temperature. With fine grain quality and favorable combining ability, 208S had no observed negative effects on fertility and agronomic traits from Bt (cry1Ab/1Ac). Additionally, 208S as a male sterile line showed no fertility decrease caused by Bt transgenic process, as it is the case in Huahui 1. Thus, 208S has great application value in two-line hybrid production for insect resistance, and can also be used as a bridge material in rice Bt transgenic breeding.

No MeSH data available.


Related in: MedlinePlus