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Development of photoperiod- and thermo-sensitive male sterility rice expressing transgene Bacillus thuringiensis.

Liu X, Zhang J, Zhang C, Wang L, Chen H, Zhu Z, Tu J - Breed. Sci. (2015)

Bottom Line: An analysis of fertility transition traits indicated that 208S was completely sterile under long day length/high temperature, but partially fertile under short day length/low temperature.With fine grain quality and favorable combining ability, 208S had no observed negative effects on fertility and agronomic traits from Bt (cry1Ab/1Ac).Additionally, 208S as a male sterile line showed no fertility decrease caused by Bt transgenic process, as it is the case in Huahui 1.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University , Yuhangtang Road 866, Hangzhou, 310058 , China.

ABSTRACT
Stem borers and leaffolders are the main pests that cause severe damage in rice (Oryza sativa L.) production worldwide. We developed the first photoperiod- and thermo-sensitive male sterility (PTSMS) rice 208S with the cry1Ab/1Ac Bacillus thuringiensis (Bt) gene, through sexual crossing with Huahui 1 (elite line with the cry1Ab/1Ac gene). The novel 208S and its hybrids presented high and stable resistance to stem borers and leaffolders, and the content of Cry1Ab/1Ac protein in chlorophyllous tissues achieved the identical level as donor and showed little accumulation in non-chlorophyllous tissue. No dominant dosage effect in the Bt gene was observed in 208S and its derived hybrids. An analysis of fertility transition traits indicated that 208S was completely sterile under long day length/high temperature, but partially fertile under short day length/low temperature. With fine grain quality and favorable combining ability, 208S had no observed negative effects on fertility and agronomic traits from Bt (cry1Ab/1Ac). Additionally, 208S as a male sterile line showed no fertility decrease caused by Bt transgenic process, as it is the case in Huahui 1. Thus, 208S has great application value in two-line hybrid production for insect resistance, and can also be used as a bridge material in rice Bt transgenic breeding.

No MeSH data available.


Related in: MedlinePlus

The resistance of control Guangzhan 63S (left) and 208S (right) against natural infection of leaffolders under field conditions without chemical control (Changxing, China, 2011).
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f1-65_333: The resistance of control Guangzhan 63S (left) and 208S (right) against natural infection of leaffolders under field conditions without chemical control (Changxing, China, 2011).

Mentions: The novel line 208S with the Bt gene showed excellent resistance against lepidopteran insects (Fig. 1). In artificial infestation tests, 208S and its hybrid suffered only 0.2% and 1.5% damage to dead heart rate, respectively, compared to 50.3% and 31.5%, respectively, in the negative controls (Fig. 2A). The results of natural infestation also showed that 208S and its hybrid achieved an identical level of resistance as that of donor parent Huahui 1, and exhibited far fewer folded leaves than the non-transgenic controls, Minghui 63 and Shanyou 63 (Fig. 2B). These results confirmed that 208S originating from cross-breeding (of Huahui 1 and Guangzhan 63S) and its hybrid offspring showed high resistance to stem borers and leaffolders.


Development of photoperiod- and thermo-sensitive male sterility rice expressing transgene Bacillus thuringiensis.

Liu X, Zhang J, Zhang C, Wang L, Chen H, Zhu Z, Tu J - Breed. Sci. (2015)

The resistance of control Guangzhan 63S (left) and 208S (right) against natural infection of leaffolders under field conditions without chemical control (Changxing, China, 2011).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542934&req=5

f1-65_333: The resistance of control Guangzhan 63S (left) and 208S (right) against natural infection of leaffolders under field conditions without chemical control (Changxing, China, 2011).
Mentions: The novel line 208S with the Bt gene showed excellent resistance against lepidopteran insects (Fig. 1). In artificial infestation tests, 208S and its hybrid suffered only 0.2% and 1.5% damage to dead heart rate, respectively, compared to 50.3% and 31.5%, respectively, in the negative controls (Fig. 2A). The results of natural infestation also showed that 208S and its hybrid achieved an identical level of resistance as that of donor parent Huahui 1, and exhibited far fewer folded leaves than the non-transgenic controls, Minghui 63 and Shanyou 63 (Fig. 2B). These results confirmed that 208S originating from cross-breeding (of Huahui 1 and Guangzhan 63S) and its hybrid offspring showed high resistance to stem borers and leaffolders.

Bottom Line: An analysis of fertility transition traits indicated that 208S was completely sterile under long day length/high temperature, but partially fertile under short day length/low temperature.With fine grain quality and favorable combining ability, 208S had no observed negative effects on fertility and agronomic traits from Bt (cry1Ab/1Ac).Additionally, 208S as a male sterile line showed no fertility decrease caused by Bt transgenic process, as it is the case in Huahui 1.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University , Yuhangtang Road 866, Hangzhou, 310058 , China.

ABSTRACT
Stem borers and leaffolders are the main pests that cause severe damage in rice (Oryza sativa L.) production worldwide. We developed the first photoperiod- and thermo-sensitive male sterility (PTSMS) rice 208S with the cry1Ab/1Ac Bacillus thuringiensis (Bt) gene, through sexual crossing with Huahui 1 (elite line with the cry1Ab/1Ac gene). The novel 208S and its hybrids presented high and stable resistance to stem borers and leaffolders, and the content of Cry1Ab/1Ac protein in chlorophyllous tissues achieved the identical level as donor and showed little accumulation in non-chlorophyllous tissue. No dominant dosage effect in the Bt gene was observed in 208S and its derived hybrids. An analysis of fertility transition traits indicated that 208S was completely sterile under long day length/high temperature, but partially fertile under short day length/low temperature. With fine grain quality and favorable combining ability, 208S had no observed negative effects on fertility and agronomic traits from Bt (cry1Ab/1Ac). Additionally, 208S as a male sterile line showed no fertility decrease caused by Bt transgenic process, as it is the case in Huahui 1. Thus, 208S has great application value in two-line hybrid production for insect resistance, and can also be used as a bridge material in rice Bt transgenic breeding.

No MeSH data available.


Related in: MedlinePlus