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Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity.

Li C, Liu C, Ma X, Wang A, Duan R, Nawrath C, Komatsuda T, Chen G - Breed. Sci. (2015)

Bottom Line: Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining.The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H.The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences , Lanzhou 730000 , China ; National Institute of Agrobiological Sciences , Tsukuba, Ibaraki 305-8602 , Japan.

ABSTRACT
The cuticle covers the aerial parts of land plants, where it serves many important functions, including water retention. Here, a recessive cuticle mutant, eceriferum-ym (cer-ym), of Hordeum vulgare L. (barley) showed abnormally glossy spikes, sheaths, and leaves. The cer-ym mutant plant detached from its root system was hypersensitive to desiccation treatment compared with wild type plants, and detached leaves of mutant lost 41.8% of their initial weight after 1 h of dehydration under laboratory conditions, while that of the wild type plants lost only 7.1%. Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining. The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H. The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.

No MeSH data available.


Related in: MedlinePlus

Genetic map of the cer-ym locus. A set of 123 OUH602 × BW-NIL (cer-ym.753) F2 progenies were analyzed with 6 genetic markers, which were previously positioned in pericentrometric region of chromosome 4H (Li et al. 2013). The genotype on cer-ym locus for each F2 line was deduced from the phenotypes of its F3 progenies.
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f3-65_327: Genetic map of the cer-ym locus. A set of 123 OUH602 × BW-NIL (cer-ym.753) F2 progenies were analyzed with 6 genetic markers, which were previously positioned in pericentrometric region of chromosome 4H (Li et al. 2013). The genotype on cer-ym locus for each F2 line was deduced from the phenotypes of its F3 progenies.

Mentions: A set of cer-zv locus-flanking markers, positioned in the pericentromeric region of chromosome 4H, were used to locate the cer-ym locus. It was shown that cer-ym mapped within a 0.8 cM region between AK370363 and AK251484, and co-segregated with AK248269 and AK364461 (Fig. 3).


Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity.

Li C, Liu C, Ma X, Wang A, Duan R, Nawrath C, Komatsuda T, Chen G - Breed. Sci. (2015)

Genetic map of the cer-ym locus. A set of 123 OUH602 × BW-NIL (cer-ym.753) F2 progenies were analyzed with 6 genetic markers, which were previously positioned in pericentrometric region of chromosome 4H (Li et al. 2013). The genotype on cer-ym locus for each F2 line was deduced from the phenotypes of its F3 progenies.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542933&req=5

f3-65_327: Genetic map of the cer-ym locus. A set of 123 OUH602 × BW-NIL (cer-ym.753) F2 progenies were analyzed with 6 genetic markers, which were previously positioned in pericentrometric region of chromosome 4H (Li et al. 2013). The genotype on cer-ym locus for each F2 line was deduced from the phenotypes of its F3 progenies.
Mentions: A set of cer-zv locus-flanking markers, positioned in the pericentromeric region of chromosome 4H, were used to locate the cer-ym locus. It was shown that cer-ym mapped within a 0.8 cM region between AK370363 and AK251484, and co-segregated with AK248269 and AK364461 (Fig. 3).

Bottom Line: Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining.The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H.The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences , Lanzhou 730000 , China ; National Institute of Agrobiological Sciences , Tsukuba, Ibaraki 305-8602 , Japan.

ABSTRACT
The cuticle covers the aerial parts of land plants, where it serves many important functions, including water retention. Here, a recessive cuticle mutant, eceriferum-ym (cer-ym), of Hordeum vulgare L. (barley) showed abnormally glossy spikes, sheaths, and leaves. The cer-ym mutant plant detached from its root system was hypersensitive to desiccation treatment compared with wild type plants, and detached leaves of mutant lost 41.8% of their initial weight after 1 h of dehydration under laboratory conditions, while that of the wild type plants lost only 7.1%. Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining. The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H. The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.

No MeSH data available.


Related in: MedlinePlus