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Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity.

Li C, Liu C, Ma X, Wang A, Duan R, Nawrath C, Komatsuda T, Chen G - Breed. Sci. (2015)

Bottom Line: Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining.The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H.The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences , Lanzhou 730000 , China ; National Institute of Agrobiological Sciences , Tsukuba, Ibaraki 305-8602 , Japan.

ABSTRACT
The cuticle covers the aerial parts of land plants, where it serves many important functions, including water retention. Here, a recessive cuticle mutant, eceriferum-ym (cer-ym), of Hordeum vulgare L. (barley) showed abnormally glossy spikes, sheaths, and leaves. The cer-ym mutant plant detached from its root system was hypersensitive to desiccation treatment compared with wild type plants, and detached leaves of mutant lost 41.8% of their initial weight after 1 h of dehydration under laboratory conditions, while that of the wild type plants lost only 7.1%. Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining. The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H. The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.

No MeSH data available.


Related in: MedlinePlus

Major cutin monomers (A) and wax component (B) of BW-NIL (cer-ym.753) and Bowman leaves. Error bars represent S.D. of biological replicates (n = 4). Two and three asterisks denote significant differences of means at P < 0.01 and P < 0.001, respectively, between Bowman and the mutant as determined by a student’s test.
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f2-65_327: Major cutin monomers (A) and wax component (B) of BW-NIL (cer-ym.753) and Bowman leaves. Error bars represent S.D. of biological replicates (n = 4). Two and three asterisks denote significant differences of means at P < 0.01 and P < 0.001, respectively, between Bowman and the mutant as determined by a student’s test.

Mentions: The cutin and waxes of the cuticle of mature leaves at the tillering stage were extracted and analyzed to help establish whether the absence of any particular component might explain the increased cuticle permeability of the cer-ym mutant. The four main cutin monomers in wild type barley leaves are: omega-hydroxy-9, 10-epoxy octadecanoic acid (ωOH-9, 10 epoxy C18); 9(10), 16-dihydroxyhexadecanoic acid (9(10), 16-OH C16); omega-hydroxyoctadecenoic acid (ωOH C18:1); and omega-hydroxyhexadecanoic acid (ωOH C16). We determined that the levels of all four monomers were substantially reduced (60%–90%) in BW-NIL (cer-ym. 753) leaves compared to Bowman leaves (Fig. 2A). In contrast, the levels of 1-hexacosanol, the main wax component, which is thought to be an important marker for wax deposition, were significantly higher (~16%) in BW-NIL (cer-ym.753) leaves compared to Bowman (Fig. 2B). Based on these results we inferred that the increased permeability of BW-NIL (cer-ym.753) leaves is caused by an overall reduction in cutin polymer deposition.


Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity.

Li C, Liu C, Ma X, Wang A, Duan R, Nawrath C, Komatsuda T, Chen G - Breed. Sci. (2015)

Major cutin monomers (A) and wax component (B) of BW-NIL (cer-ym.753) and Bowman leaves. Error bars represent S.D. of biological replicates (n = 4). Two and three asterisks denote significant differences of means at P < 0.01 and P < 0.001, respectively, between Bowman and the mutant as determined by a student’s test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542933&req=5

f2-65_327: Major cutin monomers (A) and wax component (B) of BW-NIL (cer-ym.753) and Bowman leaves. Error bars represent S.D. of biological replicates (n = 4). Two and three asterisks denote significant differences of means at P < 0.01 and P < 0.001, respectively, between Bowman and the mutant as determined by a student’s test.
Mentions: The cutin and waxes of the cuticle of mature leaves at the tillering stage were extracted and analyzed to help establish whether the absence of any particular component might explain the increased cuticle permeability of the cer-ym mutant. The four main cutin monomers in wild type barley leaves are: omega-hydroxy-9, 10-epoxy octadecanoic acid (ωOH-9, 10 epoxy C18); 9(10), 16-dihydroxyhexadecanoic acid (9(10), 16-OH C16); omega-hydroxyoctadecenoic acid (ωOH C18:1); and omega-hydroxyhexadecanoic acid (ωOH C16). We determined that the levels of all four monomers were substantially reduced (60%–90%) in BW-NIL (cer-ym. 753) leaves compared to Bowman leaves (Fig. 2A). In contrast, the levels of 1-hexacosanol, the main wax component, which is thought to be an important marker for wax deposition, were significantly higher (~16%) in BW-NIL (cer-ym.753) leaves compared to Bowman (Fig. 2B). Based on these results we inferred that the increased permeability of BW-NIL (cer-ym.753) leaves is caused by an overall reduction in cutin polymer deposition.

Bottom Line: Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining.The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H.The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences , Lanzhou 730000 , China ; National Institute of Agrobiological Sciences , Tsukuba, Ibaraki 305-8602 , Japan.

ABSTRACT
The cuticle covers the aerial parts of land plants, where it serves many important functions, including water retention. Here, a recessive cuticle mutant, eceriferum-ym (cer-ym), of Hordeum vulgare L. (barley) showed abnormally glossy spikes, sheaths, and leaves. The cer-ym mutant plant detached from its root system was hypersensitive to desiccation treatment compared with wild type plants, and detached leaves of mutant lost 41.8% of their initial weight after 1 h of dehydration under laboratory conditions, while that of the wild type plants lost only 7.1%. Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining. The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H. The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.

No MeSH data available.


Related in: MedlinePlus