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Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity.

Li C, Liu C, Ma X, Wang A, Duan R, Nawrath C, Komatsuda T, Chen G - Breed. Sci. (2015)

Bottom Line: Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining.The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H.The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences , Lanzhou 730000 , China ; National Institute of Agrobiological Sciences , Tsukuba, Ibaraki 305-8602 , Japan.

ABSTRACT
The cuticle covers the aerial parts of land plants, where it serves many important functions, including water retention. Here, a recessive cuticle mutant, eceriferum-ym (cer-ym), of Hordeum vulgare L. (barley) showed abnormally glossy spikes, sheaths, and leaves. The cer-ym mutant plant detached from its root system was hypersensitive to desiccation treatment compared with wild type plants, and detached leaves of mutant lost 41.8% of their initial weight after 1 h of dehydration under laboratory conditions, while that of the wild type plants lost only 7.1%. Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining. The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H. The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.

No MeSH data available.


Related in: MedlinePlus

Defective BW-NIL (cer-ym.753) leaf cuticle. (A) Contrasting morphologies of BW-NIL (cer-ym.753) (GSHO 2217) and Bowman. Scale bar, 10 cm. (B) Desiccation sensitivity of BW-NIL (cer-ym.753) and Bowman under laboratory conditions. Detached leaves after 3 h of drying. Scale bar, 1 cm. (C) Water loss rates of detached leaves. Error bars represent S.D. of biological replicates (n = 3). (D) Plants with the roots removed after 24 h of drying. Scale bar, 10 cm. (E) Average transpiration rate of detached leaves from BW-NIL (cer-ym.753) and Bowman under light and dark conditions. Different letters denote significant differences at P < 0.05. Error bars represent S.D. of biological replicates (n = 3 or 4). (F) Staining of leaf segments from BW-NIL (cer-ym.753) and Bowman with 0.05% (w/v) toluidine blue solution at room temperature. Scale bar, 1 cm.
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f1-65_327: Defective BW-NIL (cer-ym.753) leaf cuticle. (A) Contrasting morphologies of BW-NIL (cer-ym.753) (GSHO 2217) and Bowman. Scale bar, 10 cm. (B) Desiccation sensitivity of BW-NIL (cer-ym.753) and Bowman under laboratory conditions. Detached leaves after 3 h of drying. Scale bar, 1 cm. (C) Water loss rates of detached leaves. Error bars represent S.D. of biological replicates (n = 3). (D) Plants with the roots removed after 24 h of drying. Scale bar, 10 cm. (E) Average transpiration rate of detached leaves from BW-NIL (cer-ym.753) and Bowman under light and dark conditions. Different letters denote significant differences at P < 0.05. Error bars represent S.D. of biological replicates (n = 3 or 4). (F) Staining of leaf segments from BW-NIL (cer-ym.753) and Bowman with 0.05% (w/v) toluidine blue solution at room temperature. Scale bar, 1 cm.

Mentions: The BW-NIL (cer-ym.753) exhibited a semi-dwarf phenotype and a substantial reduction in visible wax crystals in leaf (Fig. 1A), similar to the phenotype of Bowman-near isogenic line carrying cer-zv.268 allele (GSHO 2207, BC4), named hereafter BW-NIL (cer-zv.268), which is a known cutin related recessive mutant that has a reduced water-retention capacity in its leaves (Li et al. 2013). We therefore hypothesized that the water-retention capacity might be similarly impaired in BW-NIL (cer-ym.753), and this was tested using several different experiments. When detached leaves were placed under laboratory conditions, the mutant leaves exhibited a severely desiccated appearance after 3 h of dehydration (Fig. 1B). The fresh weight of BW-NIL (cer-ym. 753) leaves decreased by 41.8 ± 3.1% after 1 h and 69.9 ± 3.8% after 2 h of dehydration, compared to the Bowman leaf weight that only decreased by 7.1 ± 0.8% and 12.7 ± 1.9%, respectively (Fig. 1C). The BW-NIL (cer-ym. 753) shoots from which the roots had been removed at the ending of the tillering stage also showed far more extensive desiccation (Fig. 1D) after 24 h of dehydration compared to the equivalent Bowman shoots, further indicating the hypersensitivity of cer-ym to desiccation.


Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity.

Li C, Liu C, Ma X, Wang A, Duan R, Nawrath C, Komatsuda T, Chen G - Breed. Sci. (2015)

Defective BW-NIL (cer-ym.753) leaf cuticle. (A) Contrasting morphologies of BW-NIL (cer-ym.753) (GSHO 2217) and Bowman. Scale bar, 10 cm. (B) Desiccation sensitivity of BW-NIL (cer-ym.753) and Bowman under laboratory conditions. Detached leaves after 3 h of drying. Scale bar, 1 cm. (C) Water loss rates of detached leaves. Error bars represent S.D. of biological replicates (n = 3). (D) Plants with the roots removed after 24 h of drying. Scale bar, 10 cm. (E) Average transpiration rate of detached leaves from BW-NIL (cer-ym.753) and Bowman under light and dark conditions. Different letters denote significant differences at P < 0.05. Error bars represent S.D. of biological replicates (n = 3 or 4). (F) Staining of leaf segments from BW-NIL (cer-ym.753) and Bowman with 0.05% (w/v) toluidine blue solution at room temperature. Scale bar, 1 cm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542933&req=5

f1-65_327: Defective BW-NIL (cer-ym.753) leaf cuticle. (A) Contrasting morphologies of BW-NIL (cer-ym.753) (GSHO 2217) and Bowman. Scale bar, 10 cm. (B) Desiccation sensitivity of BW-NIL (cer-ym.753) and Bowman under laboratory conditions. Detached leaves after 3 h of drying. Scale bar, 1 cm. (C) Water loss rates of detached leaves. Error bars represent S.D. of biological replicates (n = 3). (D) Plants with the roots removed after 24 h of drying. Scale bar, 10 cm. (E) Average transpiration rate of detached leaves from BW-NIL (cer-ym.753) and Bowman under light and dark conditions. Different letters denote significant differences at P < 0.05. Error bars represent S.D. of biological replicates (n = 3 or 4). (F) Staining of leaf segments from BW-NIL (cer-ym.753) and Bowman with 0.05% (w/v) toluidine blue solution at room temperature. Scale bar, 1 cm.
Mentions: The BW-NIL (cer-ym.753) exhibited a semi-dwarf phenotype and a substantial reduction in visible wax crystals in leaf (Fig. 1A), similar to the phenotype of Bowman-near isogenic line carrying cer-zv.268 allele (GSHO 2207, BC4), named hereafter BW-NIL (cer-zv.268), which is a known cutin related recessive mutant that has a reduced water-retention capacity in its leaves (Li et al. 2013). We therefore hypothesized that the water-retention capacity might be similarly impaired in BW-NIL (cer-ym.753), and this was tested using several different experiments. When detached leaves were placed under laboratory conditions, the mutant leaves exhibited a severely desiccated appearance after 3 h of dehydration (Fig. 1B). The fresh weight of BW-NIL (cer-ym. 753) leaves decreased by 41.8 ± 3.1% after 1 h and 69.9 ± 3.8% after 2 h of dehydration, compared to the Bowman leaf weight that only decreased by 7.1 ± 0.8% and 12.7 ± 1.9%, respectively (Fig. 1C). The BW-NIL (cer-ym. 753) shoots from which the roots had been removed at the ending of the tillering stage also showed far more extensive desiccation (Fig. 1D) after 24 h of dehydration compared to the equivalent Bowman shoots, further indicating the hypersensitivity of cer-ym to desiccation.

Bottom Line: Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining.The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H.The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences , Lanzhou 730000 , China ; National Institute of Agrobiological Sciences , Tsukuba, Ibaraki 305-8602 , Japan.

ABSTRACT
The cuticle covers the aerial parts of land plants, where it serves many important functions, including water retention. Here, a recessive cuticle mutant, eceriferum-ym (cer-ym), of Hordeum vulgare L. (barley) showed abnormally glossy spikes, sheaths, and leaves. The cer-ym mutant plant detached from its root system was hypersensitive to desiccation treatment compared with wild type plants, and detached leaves of mutant lost 41.8% of their initial weight after 1 h of dehydration under laboratory conditions, while that of the wild type plants lost only 7.1%. Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining. The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H. The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.

No MeSH data available.


Related in: MedlinePlus