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Acid detergent lignin, lodging resistance index, and expression of the caffeic acid O-methyltransferase gene in brown midrib-12 sudangrass.

Li Y, Liu G, Li J, You Y, Zhao H, Liang H, Mao P - Breed. Sci. (2015)

Bottom Line: The research showed that the mean ADL content of bmr-12 mutants (20.94 g kg(-1)) was significantly (P < 0.05) lower than measured in N-12 lines (43.45 g kg(-1)), whereas the LRI of bmr-12 mutants (0.29) was significantly (P < 0.05) higher than in N-12 lines (0.22).There was no significant correlation between the two indexes in bmr-12 materials (r = -0.44, P > 0.05).The relative expression level of COMT gene was significantly reduced, which likely led to the decreased ADL content observed in the bmr-12 mutant.

View Article: PubMed Central - PubMed

Affiliation: Department of Grassland Science, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China ; Dryland Farming Institute, Hebei Academy of Agricultural and Forestry Sciences , Hengshui 053000 , China.

ABSTRACT
Understanding the relationship between acid detergent lignin (ADL) and lodging resistance index (LRI) is essential for breeding new varieties of brown midrib (bmr) sudangrass (Sorghum sudanense (Piper) Stapf.). In this study, bmr-12 near isogenic lines and their wild-types obtained by back cross breeding were used to compare relevant forage yield and quality traits, and to analyze expression of the caffeic acid O-methyltransferase (COMT) gene using quantitative real time-PCR. The research showed that the mean ADL content of bmr-12 mutants (20.94 g kg(-1)) was significantly (P < 0.05) lower than measured in N-12 lines (43.45 g kg(-1)), whereas the LRI of bmr-12 mutants (0.29) was significantly (P < 0.05) higher than in N-12 lines (0.22). There was no significant correlation between the two indexes in bmr-12 materials (r = -0.44, P > 0.05). Sequence comparison of the COMT gene revealed two point mutations present in bmr-12 but not in the wild-type, the second mutation changed amino acid 129 of the protein from Gln (CAG) to a stop codon (UAG). The relative expression level of COMT gene was significantly reduced, which likely led to the decreased ADL content observed in the bmr-12 mutant.

No MeSH data available.


Quantitative RT-PCR analysis of the expression of the COMT gene in bmr-12 and N-12 plants from F3 populations. Relative values of COMT gene expression in the 4th internode of the main stem, including bmr-12 and N-12 lines, are shown as a percentage of COMT expression activity. Error bars represent standard deviation.
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f5-65_291: Quantitative RT-PCR analysis of the expression of the COMT gene in bmr-12 and N-12 plants from F3 populations. Relative values of COMT gene expression in the 4th internode of the main stem, including bmr-12 and N-12 lines, are shown as a percentage of COMT expression activity. Error bars represent standard deviation.

Mentions: Alignment of the COMT sequences from bmr-12 and N-12 lines revealed single base-pair changes resulting in stop codons. The SsCOMT cDNA sequence analysis revealed two C to T transition mutations in the mutant but not present in the wild-type (Fig. 4). The first mutation did not change amino acid sequence, while the second changed the 129th amino acid of the protein from Gln (CAG) to a stop codon (UAG). Thus, the mutant was presumed a allele. Meanwhile, the relative expression levels of the COMT gene between the two materials were analyzed by qRT-PCR and observed to be significantly reduced in bmr-12 compared with the N-12 lines (Fig. 5).


Acid detergent lignin, lodging resistance index, and expression of the caffeic acid O-methyltransferase gene in brown midrib-12 sudangrass.

Li Y, Liu G, Li J, You Y, Zhao H, Liang H, Mao P - Breed. Sci. (2015)

Quantitative RT-PCR analysis of the expression of the COMT gene in bmr-12 and N-12 plants from F3 populations. Relative values of COMT gene expression in the 4th internode of the main stem, including bmr-12 and N-12 lines, are shown as a percentage of COMT expression activity. Error bars represent standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542929&req=5

f5-65_291: Quantitative RT-PCR analysis of the expression of the COMT gene in bmr-12 and N-12 plants from F3 populations. Relative values of COMT gene expression in the 4th internode of the main stem, including bmr-12 and N-12 lines, are shown as a percentage of COMT expression activity. Error bars represent standard deviation.
Mentions: Alignment of the COMT sequences from bmr-12 and N-12 lines revealed single base-pair changes resulting in stop codons. The SsCOMT cDNA sequence analysis revealed two C to T transition mutations in the mutant but not present in the wild-type (Fig. 4). The first mutation did not change amino acid sequence, while the second changed the 129th amino acid of the protein from Gln (CAG) to a stop codon (UAG). Thus, the mutant was presumed a allele. Meanwhile, the relative expression levels of the COMT gene between the two materials were analyzed by qRT-PCR and observed to be significantly reduced in bmr-12 compared with the N-12 lines (Fig. 5).

Bottom Line: The research showed that the mean ADL content of bmr-12 mutants (20.94 g kg(-1)) was significantly (P < 0.05) lower than measured in N-12 lines (43.45 g kg(-1)), whereas the LRI of bmr-12 mutants (0.29) was significantly (P < 0.05) higher than in N-12 lines (0.22).There was no significant correlation between the two indexes in bmr-12 materials (r = -0.44, P > 0.05).The relative expression level of COMT gene was significantly reduced, which likely led to the decreased ADL content observed in the bmr-12 mutant.

View Article: PubMed Central - PubMed

Affiliation: Department of Grassland Science, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China ; Dryland Farming Institute, Hebei Academy of Agricultural and Forestry Sciences , Hengshui 053000 , China.

ABSTRACT
Understanding the relationship between acid detergent lignin (ADL) and lodging resistance index (LRI) is essential for breeding new varieties of brown midrib (bmr) sudangrass (Sorghum sudanense (Piper) Stapf.). In this study, bmr-12 near isogenic lines and their wild-types obtained by back cross breeding were used to compare relevant forage yield and quality traits, and to analyze expression of the caffeic acid O-methyltransferase (COMT) gene using quantitative real time-PCR. The research showed that the mean ADL content of bmr-12 mutants (20.94 g kg(-1)) was significantly (P < 0.05) lower than measured in N-12 lines (43.45 g kg(-1)), whereas the LRI of bmr-12 mutants (0.29) was significantly (P < 0.05) higher than in N-12 lines (0.22). There was no significant correlation between the two indexes in bmr-12 materials (r = -0.44, P > 0.05). Sequence comparison of the COMT gene revealed two point mutations present in bmr-12 but not in the wild-type, the second mutation changed amino acid 129 of the protein from Gln (CAG) to a stop codon (UAG). The relative expression level of COMT gene was significantly reduced, which likely led to the decreased ADL content observed in the bmr-12 mutant.

No MeSH data available.