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Acid detergent lignin, lodging resistance index, and expression of the caffeic acid O-methyltransferase gene in brown midrib-12 sudangrass.

Li Y, Liu G, Li J, You Y, Zhao H, Liang H, Mao P - Breed. Sci. (2015)

Bottom Line: The research showed that the mean ADL content of bmr-12 mutants (20.94 g kg(-1)) was significantly (P < 0.05) lower than measured in N-12 lines (43.45 g kg(-1)), whereas the LRI of bmr-12 mutants (0.29) was significantly (P < 0.05) higher than in N-12 lines (0.22).There was no significant correlation between the two indexes in bmr-12 materials (r = -0.44, P > 0.05).The relative expression level of COMT gene was significantly reduced, which likely led to the decreased ADL content observed in the bmr-12 mutant.

View Article: PubMed Central - PubMed

Affiliation: Department of Grassland Science, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China ; Dryland Farming Institute, Hebei Academy of Agricultural and Forestry Sciences , Hengshui 053000 , China.

ABSTRACT
Understanding the relationship between acid detergent lignin (ADL) and lodging resistance index (LRI) is essential for breeding new varieties of brown midrib (bmr) sudangrass (Sorghum sudanense (Piper) Stapf.). In this study, bmr-12 near isogenic lines and their wild-types obtained by back cross breeding were used to compare relevant forage yield and quality traits, and to analyze expression of the caffeic acid O-methyltransferase (COMT) gene using quantitative real time-PCR. The research showed that the mean ADL content of bmr-12 mutants (20.94 g kg(-1)) was significantly (P < 0.05) lower than measured in N-12 lines (43.45 g kg(-1)), whereas the LRI of bmr-12 mutants (0.29) was significantly (P < 0.05) higher than in N-12 lines (0.22). There was no significant correlation between the two indexes in bmr-12 materials (r = -0.44, P > 0.05). Sequence comparison of the COMT gene revealed two point mutations present in bmr-12 but not in the wild-type, the second mutation changed amino acid 129 of the protein from Gln (CAG) to a stop codon (UAG). The relative expression level of COMT gene was significantly reduced, which likely led to the decreased ADL content observed in the bmr-12 mutant.

No MeSH data available.


Phylogenetic tree constructed using SsCOMT sequences and other COMT proteins. Thirteen-residue sequences were aligned and a phylogenetic tree constructed using the neighbor-joining method. Species used: S. sudanense (SsCOMT), S. bicolor (SbCOMT), Zea mays (ZmCOMT), Panicum virgatum (PvCOMT), Oryza sativa Japonica Group (OsCOMT), Triticum aestivum (TaCOMT), Festuca arundinacea (FaCOMT), Arabidopsis thaliana (AtCOMT1), Gossypium hirsutum (GhCOMT1), Betula platyphylla (BpCOMT), Medicago sativa (MsCOMT), Populus trichocarpa (PtCOMT1), and Selaginella moellendorffii (SmCOMT). GenBank accession numbers are indicated. The reliability of the tree was measured by bootstrap analysis.
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f3-65_291: Phylogenetic tree constructed using SsCOMT sequences and other COMT proteins. Thirteen-residue sequences were aligned and a phylogenetic tree constructed using the neighbor-joining method. Species used: S. sudanense (SsCOMT), S. bicolor (SbCOMT), Zea mays (ZmCOMT), Panicum virgatum (PvCOMT), Oryza sativa Japonica Group (OsCOMT), Triticum aestivum (TaCOMT), Festuca arundinacea (FaCOMT), Arabidopsis thaliana (AtCOMT1), Gossypium hirsutum (GhCOMT1), Betula platyphylla (BpCOMT), Medicago sativa (MsCOMT), Populus trichocarpa (PtCOMT1), and Selaginella moellendorffii (SmCOMT). GenBank accession numbers are indicated. The reliability of the tree was measured by bootstrap analysis.

Mentions: Based on the amino acid sequence, a phylogenetic tree was constructed by using neighbor-joining method. The result indicated that SsCOMT was homologous with monocotyledonous species. SsCOMT showed 100% amino acid sequence identity to SbCOMT, 89.89% to ZmCOMT, 80.5% to OsCOMT, 79.06% to TaCOMT, and 60.6% to AtCOMT (Fig. 3).


Acid detergent lignin, lodging resistance index, and expression of the caffeic acid O-methyltransferase gene in brown midrib-12 sudangrass.

Li Y, Liu G, Li J, You Y, Zhao H, Liang H, Mao P - Breed. Sci. (2015)

Phylogenetic tree constructed using SsCOMT sequences and other COMT proteins. Thirteen-residue sequences were aligned and a phylogenetic tree constructed using the neighbor-joining method. Species used: S. sudanense (SsCOMT), S. bicolor (SbCOMT), Zea mays (ZmCOMT), Panicum virgatum (PvCOMT), Oryza sativa Japonica Group (OsCOMT), Triticum aestivum (TaCOMT), Festuca arundinacea (FaCOMT), Arabidopsis thaliana (AtCOMT1), Gossypium hirsutum (GhCOMT1), Betula platyphylla (BpCOMT), Medicago sativa (MsCOMT), Populus trichocarpa (PtCOMT1), and Selaginella moellendorffii (SmCOMT). GenBank accession numbers are indicated. The reliability of the tree was measured by bootstrap analysis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542929&req=5

f3-65_291: Phylogenetic tree constructed using SsCOMT sequences and other COMT proteins. Thirteen-residue sequences were aligned and a phylogenetic tree constructed using the neighbor-joining method. Species used: S. sudanense (SsCOMT), S. bicolor (SbCOMT), Zea mays (ZmCOMT), Panicum virgatum (PvCOMT), Oryza sativa Japonica Group (OsCOMT), Triticum aestivum (TaCOMT), Festuca arundinacea (FaCOMT), Arabidopsis thaliana (AtCOMT1), Gossypium hirsutum (GhCOMT1), Betula platyphylla (BpCOMT), Medicago sativa (MsCOMT), Populus trichocarpa (PtCOMT1), and Selaginella moellendorffii (SmCOMT). GenBank accession numbers are indicated. The reliability of the tree was measured by bootstrap analysis.
Mentions: Based on the amino acid sequence, a phylogenetic tree was constructed by using neighbor-joining method. The result indicated that SsCOMT was homologous with monocotyledonous species. SsCOMT showed 100% amino acid sequence identity to SbCOMT, 89.89% to ZmCOMT, 80.5% to OsCOMT, 79.06% to TaCOMT, and 60.6% to AtCOMT (Fig. 3).

Bottom Line: The research showed that the mean ADL content of bmr-12 mutants (20.94 g kg(-1)) was significantly (P < 0.05) lower than measured in N-12 lines (43.45 g kg(-1)), whereas the LRI of bmr-12 mutants (0.29) was significantly (P < 0.05) higher than in N-12 lines (0.22).There was no significant correlation between the two indexes in bmr-12 materials (r = -0.44, P > 0.05).The relative expression level of COMT gene was significantly reduced, which likely led to the decreased ADL content observed in the bmr-12 mutant.

View Article: PubMed Central - PubMed

Affiliation: Department of Grassland Science, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China ; Dryland Farming Institute, Hebei Academy of Agricultural and Forestry Sciences , Hengshui 053000 , China.

ABSTRACT
Understanding the relationship between acid detergent lignin (ADL) and lodging resistance index (LRI) is essential for breeding new varieties of brown midrib (bmr) sudangrass (Sorghum sudanense (Piper) Stapf.). In this study, bmr-12 near isogenic lines and their wild-types obtained by back cross breeding were used to compare relevant forage yield and quality traits, and to analyze expression of the caffeic acid O-methyltransferase (COMT) gene using quantitative real time-PCR. The research showed that the mean ADL content of bmr-12 mutants (20.94 g kg(-1)) was significantly (P < 0.05) lower than measured in N-12 lines (43.45 g kg(-1)), whereas the LRI of bmr-12 mutants (0.29) was significantly (P < 0.05) higher than in N-12 lines (0.22). There was no significant correlation between the two indexes in bmr-12 materials (r = -0.44, P > 0.05). Sequence comparison of the COMT gene revealed two point mutations present in bmr-12 but not in the wild-type, the second mutation changed amino acid 129 of the protein from Gln (CAG) to a stop codon (UAG). The relative expression level of COMT gene was significantly reduced, which likely led to the decreased ADL content observed in the bmr-12 mutant.

No MeSH data available.