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Acid detergent lignin, lodging resistance index, and expression of the caffeic acid O-methyltransferase gene in brown midrib-12 sudangrass.

Li Y, Liu G, Li J, You Y, Zhao H, Liang H, Mao P - Breed. Sci. (2015)

Bottom Line: The research showed that the mean ADL content of bmr-12 mutants (20.94 g kg(-1)) was significantly (P < 0.05) lower than measured in N-12 lines (43.45 g kg(-1)), whereas the LRI of bmr-12 mutants (0.29) was significantly (P < 0.05) higher than in N-12 lines (0.22).There was no significant correlation between the two indexes in bmr-12 materials (r = -0.44, P > 0.05).The relative expression level of COMT gene was significantly reduced, which likely led to the decreased ADL content observed in the bmr-12 mutant.

View Article: PubMed Central - PubMed

Affiliation: Department of Grassland Science, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China ; Dryland Farming Institute, Hebei Academy of Agricultural and Forestry Sciences , Hengshui 053000 , China.

ABSTRACT
Understanding the relationship between acid detergent lignin (ADL) and lodging resistance index (LRI) is essential for breeding new varieties of brown midrib (bmr) sudangrass (Sorghum sudanense (Piper) Stapf.). In this study, bmr-12 near isogenic lines and their wild-types obtained by back cross breeding were used to compare relevant forage yield and quality traits, and to analyze expression of the caffeic acid O-methyltransferase (COMT) gene using quantitative real time-PCR. The research showed that the mean ADL content of bmr-12 mutants (20.94 g kg(-1)) was significantly (P < 0.05) lower than measured in N-12 lines (43.45 g kg(-1)), whereas the LRI of bmr-12 mutants (0.29) was significantly (P < 0.05) higher than in N-12 lines (0.22). There was no significant correlation between the two indexes in bmr-12 materials (r = -0.44, P > 0.05). Sequence comparison of the COMT gene revealed two point mutations present in bmr-12 but not in the wild-type, the second mutation changed amino acid 129 of the protein from Gln (CAG) to a stop codon (UAG). The relative expression level of COMT gene was significantly reduced, which likely led to the decreased ADL content observed in the bmr-12 mutant.

No MeSH data available.


The bmr-12 (A) and N-12 plant materials (B) after 122 days of growth in southeast Hebei province. There were obvious differences in coloration of the stem and midribs of N-12 (C and F) and bmr-12 materials (D and E).
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f1-65_291: The bmr-12 (A) and N-12 plant materials (B) after 122 days of growth in southeast Hebei province. There were obvious differences in coloration of the stem and midribs of N-12 (C and F) and bmr-12 materials (D and E).

Mentions: A pair of bmr-12 near isogenic lines (bmr-12 vs N-12) from F3 sudangrass populations was used in this study. The near-isogenic lines share similar characteristics for main stem growth period, plant height, leaf number, and stem diameter, but have differences in leaf midrib color (Fig. 1). They were purposefully screened and bagged from self-pollinated F1 and F2 descendants of a hybrid between S2006 (female parent, a green-midrib strain), and B4145 (male parent, a brown midrib-12 line). Their parents were derived from forage sorghum hybrids from China and the United States, and which obtained by six individual selections and three bulk selections, followed by two years of adaptability evaluation, from 2004 to 2011, respectively.


Acid detergent lignin, lodging resistance index, and expression of the caffeic acid O-methyltransferase gene in brown midrib-12 sudangrass.

Li Y, Liu G, Li J, You Y, Zhao H, Liang H, Mao P - Breed. Sci. (2015)

The bmr-12 (A) and N-12 plant materials (B) after 122 days of growth in southeast Hebei province. There were obvious differences in coloration of the stem and midribs of N-12 (C and F) and bmr-12 materials (D and E).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542929&req=5

f1-65_291: The bmr-12 (A) and N-12 plant materials (B) after 122 days of growth in southeast Hebei province. There were obvious differences in coloration of the stem and midribs of N-12 (C and F) and bmr-12 materials (D and E).
Mentions: A pair of bmr-12 near isogenic lines (bmr-12 vs N-12) from F3 sudangrass populations was used in this study. The near-isogenic lines share similar characteristics for main stem growth period, plant height, leaf number, and stem diameter, but have differences in leaf midrib color (Fig. 1). They were purposefully screened and bagged from self-pollinated F1 and F2 descendants of a hybrid between S2006 (female parent, a green-midrib strain), and B4145 (male parent, a brown midrib-12 line). Their parents were derived from forage sorghum hybrids from China and the United States, and which obtained by six individual selections and three bulk selections, followed by two years of adaptability evaluation, from 2004 to 2011, respectively.

Bottom Line: The research showed that the mean ADL content of bmr-12 mutants (20.94 g kg(-1)) was significantly (P < 0.05) lower than measured in N-12 lines (43.45 g kg(-1)), whereas the LRI of bmr-12 mutants (0.29) was significantly (P < 0.05) higher than in N-12 lines (0.22).There was no significant correlation between the two indexes in bmr-12 materials (r = -0.44, P > 0.05).The relative expression level of COMT gene was significantly reduced, which likely led to the decreased ADL content observed in the bmr-12 mutant.

View Article: PubMed Central - PubMed

Affiliation: Department of Grassland Science, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China ; Dryland Farming Institute, Hebei Academy of Agricultural and Forestry Sciences , Hengshui 053000 , China.

ABSTRACT
Understanding the relationship between acid detergent lignin (ADL) and lodging resistance index (LRI) is essential for breeding new varieties of brown midrib (bmr) sudangrass (Sorghum sudanense (Piper) Stapf.). In this study, bmr-12 near isogenic lines and their wild-types obtained by back cross breeding were used to compare relevant forage yield and quality traits, and to analyze expression of the caffeic acid O-methyltransferase (COMT) gene using quantitative real time-PCR. The research showed that the mean ADL content of bmr-12 mutants (20.94 g kg(-1)) was significantly (P < 0.05) lower than measured in N-12 lines (43.45 g kg(-1)), whereas the LRI of bmr-12 mutants (0.29) was significantly (P < 0.05) higher than in N-12 lines (0.22). There was no significant correlation between the two indexes in bmr-12 materials (r = -0.44, P > 0.05). Sequence comparison of the COMT gene revealed two point mutations present in bmr-12 but not in the wild-type, the second mutation changed amino acid 129 of the protein from Gln (CAG) to a stop codon (UAG). The relative expression level of COMT gene was significantly reduced, which likely led to the decreased ADL content observed in the bmr-12 mutant.

No MeSH data available.