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Sampling blood from the lateral tail vein of the rat.

Lee G, Goosens KA - J Vis Exp (2015)

Bottom Line: One factor that can change the blood composition is the stress response triggered by the sampling procedure, which can contribute to variability in the measures of interest.This procedure offers significant advantages over other more commonly used techniques.Additionally, it can be used to obtain large volume samples (upwards of 1 ml in some rats), and it may be used repeatedly across experimental days.

View Article: PubMed Central - PubMed

Affiliation: McGovern Institute for Brain Research, Massachusetts Institute of Technology.

ABSTRACT
Blood samples are commonly obtained in many experimental contexts to measure targets of interest, including hormones, immune factors, growth factors, proteins, and glucose, yet the composition of the blood is dynamically regulated and easily perturbed. One factor that can change the blood composition is the stress response triggered by the sampling procedure, which can contribute to variability in the measures of interest. Here we describe a procedure for blood sampling from the lateral tail vein in the rat. This procedure offers significant advantages over other more commonly used techniques. It permits rapid sampling with minimal pain or invasiveness, without anesthesia or analgesia. Additionally, it can be used to obtain large volume samples (upwards of 1 ml in some rats), and it may be used repeatedly across experimental days. By minimizing the stress response and pain resulting from blood sampling, measures can more accurately reflect the true basal state of the animal, with minimal influence from the sampling procedure itself.

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Sampling blood from the lateral tail vein of the rat.

Lee G, Goosens KA - J Vis Exp (2015)

© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542852&req=5

Bottom Line: One factor that can change the blood composition is the stress response triggered by the sampling procedure, which can contribute to variability in the measures of interest.This procedure offers significant advantages over other more commonly used techniques.Additionally, it can be used to obtain large volume samples (upwards of 1 ml in some rats), and it may be used repeatedly across experimental days.

View Article: PubMed Central - PubMed

Affiliation: McGovern Institute for Brain Research, Massachusetts Institute of Technology.

ABSTRACT
Blood samples are commonly obtained in many experimental contexts to measure targets of interest, including hormones, immune factors, growth factors, proteins, and glucose, yet the composition of the blood is dynamically regulated and easily perturbed. One factor that can change the blood composition is the stress response triggered by the sampling procedure, which can contribute to variability in the measures of interest. Here we describe a procedure for blood sampling from the lateral tail vein in the rat. This procedure offers significant advantages over other more commonly used techniques. It permits rapid sampling with minimal pain or invasiveness, without anesthesia or analgesia. Additionally, it can be used to obtain large volume samples (upwards of 1 ml in some rats), and it may be used repeatedly across experimental days. By minimizing the stress response and pain resulting from blood sampling, measures can more accurately reflect the true basal state of the animal, with minimal influence from the sampling procedure itself.

Show MeSH
Related in: MedlinePlus