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De novo assembly of the Japanese lawngrass (Zoysia japonica Steud.) root transcriptome and identification of candidate unigenes related to early responses under salt stress.

Xie Q, Niu J, Xu X, Xu L, Zhang Y, Fan B, Liang X, Zhang L, Yin S, Han L - Front Plant Sci (2015)

Bottom Line: Compared with the control, 6035 genes were significantly different (false discovery rate ≤0.01, /log2Ratio/≥1) in the NaCl-treated samples.Using high-throughput next-generation sequencing, we built a database as a global transcript resource for Z. japonica Steud. roots.The results of this study will advance our understanding of the early salt response in Japanese lawngrass roots.

View Article: PubMed Central - PubMed

Affiliation: Institute of Turfgrass Science, College of Forestry, Beijing Forestry University Beijing, China.

ABSTRACT
Japanese lawngrass (Zoysia japonica Steud.) is an important warm-season turfgrass that is able to survive in a range of soils, from infertile sands to clays, and to grow well under saline conditions. However, little is known about the molecular mechanisms involved in its resistance to salt stress. Here, we used high-throughput RNA sequencing (RNA-seq) to investigate the changes in gene expression of Zoysia grass at high NaCl concentrations. We first constructed two sequencing libraries, including control and NaCl-treated samples, and sequenced them using the Illumina HiSeq™ 2000 platform. Approximately 157.20 million paired-end reads with a total length of 68.68 Mb were obtained. Subsequently, 100,800 unigenes with an N50 length of 1104 bp were assembled using Trinity, among which 70,127 unigenes were functionally annotated (E ≤ 10(-5)) in the non-redundant protein (NR) database. Furthermore, three public databases, the Kyoto Encyclopedia of Genes and Genomes (KEGG), Swiss-prot, and Clusters of Orthologous Groups (COGs), were used for gene function analysis and enrichment. The annotated genes included 46 Gene Ontology (GO) terms, 120 KEGG pathways, and 25 COGs. Compared with the control, 6035 genes were significantly different (false discovery rate ≤0.01, /log2Ratio/≥1) in the NaCl-treated samples. These genes were enriched in 10 KEGG pathways and 58 GO terms, and subjected to 25 COG categories. Using high-throughput next-generation sequencing, we built a database as a global transcript resource for Z. japonica Steud. roots. The results of this study will advance our understanding of the early salt response in Japanese lawngrass roots.

No MeSH data available.


Related in: MedlinePlus

Sample roots were treated with DAB to detect H2O2 in the dark for 4 h. CK, Plants grown under normal conditions produced little H2O2; 10 min, in plants treated with salt for 10 min, the H2O2 level was slightly increased in lateral roots, while H2O2 was also detected in the main root; 20 min, in plants treated with salt for 20 min, H2O2 accumulation was detected in the entire root; 30 min, in plants treated with salt for 30 min, the roots were submerged in H2O2.
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Figure 1: Sample roots were treated with DAB to detect H2O2 in the dark for 4 h. CK, Plants grown under normal conditions produced little H2O2; 10 min, in plants treated with salt for 10 min, the H2O2 level was slightly increased in lateral roots, while H2O2 was also detected in the main root; 20 min, in plants treated with salt for 20 min, H2O2 accumulation was detected in the entire root; 30 min, in plants treated with salt for 30 min, the roots were submerged in H2O2.

Mentions: As shown in Figure 1, the H2O2 concentration in roots increased shortly after salt treatment. Compared with the control plants, a yellow substance was visible on the roots. After 30 min of salt treatment, oxidative stress caused by H2O2 was visible throughout the roots. Thus, we selected representative samples at 0 and 30 min for transcriptomic sequencing.


De novo assembly of the Japanese lawngrass (Zoysia japonica Steud.) root transcriptome and identification of candidate unigenes related to early responses under salt stress.

Xie Q, Niu J, Xu X, Xu L, Zhang Y, Fan B, Liang X, Zhang L, Yin S, Han L - Front Plant Sci (2015)

Sample roots were treated with DAB to detect H2O2 in the dark for 4 h. CK, Plants grown under normal conditions produced little H2O2; 10 min, in plants treated with salt for 10 min, the H2O2 level was slightly increased in lateral roots, while H2O2 was also detected in the main root; 20 min, in plants treated with salt for 20 min, H2O2 accumulation was detected in the entire root; 30 min, in plants treated with salt for 30 min, the roots were submerged in H2O2.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542685&req=5

Figure 1: Sample roots were treated with DAB to detect H2O2 in the dark for 4 h. CK, Plants grown under normal conditions produced little H2O2; 10 min, in plants treated with salt for 10 min, the H2O2 level was slightly increased in lateral roots, while H2O2 was also detected in the main root; 20 min, in plants treated with salt for 20 min, H2O2 accumulation was detected in the entire root; 30 min, in plants treated with salt for 30 min, the roots were submerged in H2O2.
Mentions: As shown in Figure 1, the H2O2 concentration in roots increased shortly after salt treatment. Compared with the control plants, a yellow substance was visible on the roots. After 30 min of salt treatment, oxidative stress caused by H2O2 was visible throughout the roots. Thus, we selected representative samples at 0 and 30 min for transcriptomic sequencing.

Bottom Line: Compared with the control, 6035 genes were significantly different (false discovery rate ≤0.01, /log2Ratio/≥1) in the NaCl-treated samples.Using high-throughput next-generation sequencing, we built a database as a global transcript resource for Z. japonica Steud. roots.The results of this study will advance our understanding of the early salt response in Japanese lawngrass roots.

View Article: PubMed Central - PubMed

Affiliation: Institute of Turfgrass Science, College of Forestry, Beijing Forestry University Beijing, China.

ABSTRACT
Japanese lawngrass (Zoysia japonica Steud.) is an important warm-season turfgrass that is able to survive in a range of soils, from infertile sands to clays, and to grow well under saline conditions. However, little is known about the molecular mechanisms involved in its resistance to salt stress. Here, we used high-throughput RNA sequencing (RNA-seq) to investigate the changes in gene expression of Zoysia grass at high NaCl concentrations. We first constructed two sequencing libraries, including control and NaCl-treated samples, and sequenced them using the Illumina HiSeq™ 2000 platform. Approximately 157.20 million paired-end reads with a total length of 68.68 Mb were obtained. Subsequently, 100,800 unigenes with an N50 length of 1104 bp were assembled using Trinity, among which 70,127 unigenes were functionally annotated (E ≤ 10(-5)) in the non-redundant protein (NR) database. Furthermore, three public databases, the Kyoto Encyclopedia of Genes and Genomes (KEGG), Swiss-prot, and Clusters of Orthologous Groups (COGs), were used for gene function analysis and enrichment. The annotated genes included 46 Gene Ontology (GO) terms, 120 KEGG pathways, and 25 COGs. Compared with the control, 6035 genes were significantly different (false discovery rate ≤0.01, /log2Ratio/≥1) in the NaCl-treated samples. These genes were enriched in 10 KEGG pathways and 58 GO terms, and subjected to 25 COG categories. Using high-throughput next-generation sequencing, we built a database as a global transcript resource for Z. japonica Steud. roots. The results of this study will advance our understanding of the early salt response in Japanese lawngrass roots.

No MeSH data available.


Related in: MedlinePlus