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Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering.

Braghirolli DI, Zamboni F, Acasigua GA, Pranke P - Int J Nanomedicine (2015)

Bottom Line: Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds.SCCs exhibited good mechanical properties, compatible with their handling and further implantation.The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Hematology and Stem Cells Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil ; Department of Materials Science, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.

ABSTRACT
In tissue engineering, a uniform cell occupation of scaffolds is crucial to ensure the success of tissue regeneration. However, this point remains an unsolved problem in 3D scaffolds. In this study, a direct method to integrate cells into fiber scaffolds was investigated by combining the methods of electrospinning of fibers and bioelectrospraying of cells. With the associating of these methods, the cells were incorporated into the 3D scaffolds while the fibers were being produced. The scaffolds containing cells (SCCs) were produced using 20% poly(lactide-co-glycolide) solution for electrospinning and mesenchymal stem cells from deciduous teeth as a suspension for bioelectrospraying. After their production, the SCCs were cultivated for 15 days at 37°C with an atmosphere of 5% CO2. The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide test demonstrated that the cells remained viable and were able to grow between the fibers. Scanning electron microscopy showed the presence of a high number of cells in the structure of the scaffolds and confocal images demonstrated that the cells were able to adapt and spread between the fibers. Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds. Some physicochemical properties of the scaffolds were also investigated. SCCs exhibited good mechanical properties, compatible with their handling and further implantation. The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

No MeSH data available.


Analysis of cell viability using the MTT test in scaffolds containing cells.Notes: Results are shown as mean ± standard error. *Shows statistical difference between the groups (P<0.05) according to the ANOVA test followed by the Tukey posttest.Abbreviations: ANOVA, analysis of variance; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; h, hours.
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f7-ijn-10-5159: Analysis of cell viability using the MTT test in scaffolds containing cells.Notes: Results are shown as mean ± standard error. *Shows statistical difference between the groups (P<0.05) according to the ANOVA test followed by the Tukey posttest.Abbreviations: ANOVA, analysis of variance; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; h, hours.

Mentions: Cell viability after bioelectrospraying (without its association with fiber electrospinning) was 93%±4.4%. Meanwhile, cell viability after the bioelectrospraying and electrospinning procedures was 89%±4.6%. The initial density of viable MSCs in the SCC structure was estimated using the MTT test by comparison of the absorbance of the sample with that of the standard (Figure 7). The average number of viable cells integrated between the fibers was (5.039±1.008)×103 cells/mm3.


Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering.

Braghirolli DI, Zamboni F, Acasigua GA, Pranke P - Int J Nanomedicine (2015)

Analysis of cell viability using the MTT test in scaffolds containing cells.Notes: Results are shown as mean ± standard error. *Shows statistical difference between the groups (P<0.05) according to the ANOVA test followed by the Tukey posttest.Abbreviations: ANOVA, analysis of variance; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; h, hours.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542624&req=5

f7-ijn-10-5159: Analysis of cell viability using the MTT test in scaffolds containing cells.Notes: Results are shown as mean ± standard error. *Shows statistical difference between the groups (P<0.05) according to the ANOVA test followed by the Tukey posttest.Abbreviations: ANOVA, analysis of variance; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; h, hours.
Mentions: Cell viability after bioelectrospraying (without its association with fiber electrospinning) was 93%±4.4%. Meanwhile, cell viability after the bioelectrospraying and electrospinning procedures was 89%±4.6%. The initial density of viable MSCs in the SCC structure was estimated using the MTT test by comparison of the absorbance of the sample with that of the standard (Figure 7). The average number of viable cells integrated between the fibers was (5.039±1.008)×103 cells/mm3.

Bottom Line: Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds.SCCs exhibited good mechanical properties, compatible with their handling and further implantation.The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Hematology and Stem Cells Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil ; Department of Materials Science, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.

ABSTRACT
In tissue engineering, a uniform cell occupation of scaffolds is crucial to ensure the success of tissue regeneration. However, this point remains an unsolved problem in 3D scaffolds. In this study, a direct method to integrate cells into fiber scaffolds was investigated by combining the methods of electrospinning of fibers and bioelectrospraying of cells. With the associating of these methods, the cells were incorporated into the 3D scaffolds while the fibers were being produced. The scaffolds containing cells (SCCs) were produced using 20% poly(lactide-co-glycolide) solution for electrospinning and mesenchymal stem cells from deciduous teeth as a suspension for bioelectrospraying. After their production, the SCCs were cultivated for 15 days at 37°C with an atmosphere of 5% CO2. The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide test demonstrated that the cells remained viable and were able to grow between the fibers. Scanning electron microscopy showed the presence of a high number of cells in the structure of the scaffolds and confocal images demonstrated that the cells were able to adapt and spread between the fibers. Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds. Some physicochemical properties of the scaffolds were also investigated. SCCs exhibited good mechanical properties, compatible with their handling and further implantation. The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

No MeSH data available.