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Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering.

Braghirolli DI, Zamboni F, Acasigua GA, Pranke P - Int J Nanomedicine (2015)

Bottom Line: Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds.SCCs exhibited good mechanical properties, compatible with their handling and further implantation.The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Hematology and Stem Cells Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil ; Department of Materials Science, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.

ABSTRACT
In tissue engineering, a uniform cell occupation of scaffolds is crucial to ensure the success of tissue regeneration. However, this point remains an unsolved problem in 3D scaffolds. In this study, a direct method to integrate cells into fiber scaffolds was investigated by combining the methods of electrospinning of fibers and bioelectrospraying of cells. With the associating of these methods, the cells were incorporated into the 3D scaffolds while the fibers were being produced. The scaffolds containing cells (SCCs) were produced using 20% poly(lactide-co-glycolide) solution for electrospinning and mesenchymal stem cells from deciduous teeth as a suspension for bioelectrospraying. After their production, the SCCs were cultivated for 15 days at 37°C with an atmosphere of 5% CO2. The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide test demonstrated that the cells remained viable and were able to grow between the fibers. Scanning electron microscopy showed the presence of a high number of cells in the structure of the scaffolds and confocal images demonstrated that the cells were able to adapt and spread between the fibers. Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds. Some physicochemical properties of the scaffolds were also investigated. SCCs exhibited good mechanical properties, compatible with their handling and further implantation. The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

No MeSH data available.


Sample of scaffold containing cells after production.Note: The arrow shows the more central localization of the electrospraying jet through the width of the scaffold.
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f2-ijn-10-5159: Sample of scaffold containing cells after production.Note: The arrow shows the more central localization of the electrospraying jet through the width of the scaffold.

Mentions: SCCs were produced by the association of the bioelectrospraying and electrospinning methods (Figure 2). Cellular suspensions with concentrations ranging from 3×106 cells/mL to 7.5×106 cells/mL could be electrosprayed successfully without the need for changes in the bioelectrospraying parameters. The suspension with concentration 7.5×106 cells/mL was chosen for SCC production. During SCC formation, it was observed that the direction of the electrospraying jet was in a central position in the fiber area formed by electrospinning. Because of this, the electrospraying bomb was rotated every 5 minutes to increase the bioelectrospraying and electrospinning convergence area, creating a more homogeneous cellular distribution among the electrospun fibers. After production of SCCs, their physicochemical and biological properties were evaluated.


Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering.

Braghirolli DI, Zamboni F, Acasigua GA, Pranke P - Int J Nanomedicine (2015)

Sample of scaffold containing cells after production.Note: The arrow shows the more central localization of the electrospraying jet through the width of the scaffold.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542624&req=5

f2-ijn-10-5159: Sample of scaffold containing cells after production.Note: The arrow shows the more central localization of the electrospraying jet through the width of the scaffold.
Mentions: SCCs were produced by the association of the bioelectrospraying and electrospinning methods (Figure 2). Cellular suspensions with concentrations ranging from 3×106 cells/mL to 7.5×106 cells/mL could be electrosprayed successfully without the need for changes in the bioelectrospraying parameters. The suspension with concentration 7.5×106 cells/mL was chosen for SCC production. During SCC formation, it was observed that the direction of the electrospraying jet was in a central position in the fiber area formed by electrospinning. Because of this, the electrospraying bomb was rotated every 5 minutes to increase the bioelectrospraying and electrospinning convergence area, creating a more homogeneous cellular distribution among the electrospun fibers. After production of SCCs, their physicochemical and biological properties were evaluated.

Bottom Line: Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds.SCCs exhibited good mechanical properties, compatible with their handling and further implantation.The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Hematology and Stem Cells Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil ; Department of Materials Science, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.

ABSTRACT
In tissue engineering, a uniform cell occupation of scaffolds is crucial to ensure the success of tissue regeneration. However, this point remains an unsolved problem in 3D scaffolds. In this study, a direct method to integrate cells into fiber scaffolds was investigated by combining the methods of electrospinning of fibers and bioelectrospraying of cells. With the associating of these methods, the cells were incorporated into the 3D scaffolds while the fibers were being produced. The scaffolds containing cells (SCCs) were produced using 20% poly(lactide-co-glycolide) solution for electrospinning and mesenchymal stem cells from deciduous teeth as a suspension for bioelectrospraying. After their production, the SCCs were cultivated for 15 days at 37°C with an atmosphere of 5% CO2. The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide test demonstrated that the cells remained viable and were able to grow between the fibers. Scanning electron microscopy showed the presence of a high number of cells in the structure of the scaffolds and confocal images demonstrated that the cells were able to adapt and spread between the fibers. Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds. Some physicochemical properties of the scaffolds were also investigated. SCCs exhibited good mechanical properties, compatible with their handling and further implantation. The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

No MeSH data available.