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Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering.

Braghirolli DI, Zamboni F, Acasigua GA, Pranke P - Int J Nanomedicine (2015)

Bottom Line: Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds.SCCs exhibited good mechanical properties, compatible with their handling and further implantation.The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Hematology and Stem Cells Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil ; Department of Materials Science, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.

ABSTRACT
In tissue engineering, a uniform cell occupation of scaffolds is crucial to ensure the success of tissue regeneration. However, this point remains an unsolved problem in 3D scaffolds. In this study, a direct method to integrate cells into fiber scaffolds was investigated by combining the methods of electrospinning of fibers and bioelectrospraying of cells. With the associating of these methods, the cells were incorporated into the 3D scaffolds while the fibers were being produced. The scaffolds containing cells (SCCs) were produced using 20% poly(lactide-co-glycolide) solution for electrospinning and mesenchymal stem cells from deciduous teeth as a suspension for bioelectrospraying. After their production, the SCCs were cultivated for 15 days at 37°C with an atmosphere of 5% CO2. The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide test demonstrated that the cells remained viable and were able to grow between the fibers. Scanning electron microscopy showed the presence of a high number of cells in the structure of the scaffolds and confocal images demonstrated that the cells were able to adapt and spread between the fibers. Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds. Some physicochemical properties of the scaffolds were also investigated. SCCs exhibited good mechanical properties, compatible with their handling and further implantation. The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

No MeSH data available.


Histological cross-sections of scaffolds containing cells after (A) 1 day of cultivation and (B) 15 days of cultivation.Notes: (A) Original magnifications: ×200; (B) original magnifications: ×100. Scale bars represent 50 um.
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f10-ijn-10-5159: Histological cross-sections of scaffolds containing cells after (A) 1 day of cultivation and (B) 15 days of cultivation.Notes: (A) Original magnifications: ×200; (B) original magnifications: ×100. Scale bars represent 50 um.

Mentions: Cross-sections of paraffin-embedded SCCs after 1 day of cultivation showed that the MSCs were uniformly distributed throughout the thickness of the scaffolds (Figure 10A). The images from the middle and from the right and left ends of the SCCs prove that a pronounced integration of cells occurred in the central portion of the SCCs. In the middle of the scaffolds, a larger number of cells could be visualized, while in the outermost portions, lesser number of cells was found. However, after 15 days of cultivation, the cross-sections of the SCCs demonstrated an increase in the number of cells and an improved filling of the scaffold structure by the MSCs (Figure 10B). The cells were able to proliferate and fill the ends of the SSCs. No difference was observed between the different portions of the scaffolds.


Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering.

Braghirolli DI, Zamboni F, Acasigua GA, Pranke P - Int J Nanomedicine (2015)

Histological cross-sections of scaffolds containing cells after (A) 1 day of cultivation and (B) 15 days of cultivation.Notes: (A) Original magnifications: ×200; (B) original magnifications: ×100. Scale bars represent 50 um.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542624&req=5

f10-ijn-10-5159: Histological cross-sections of scaffolds containing cells after (A) 1 day of cultivation and (B) 15 days of cultivation.Notes: (A) Original magnifications: ×200; (B) original magnifications: ×100. Scale bars represent 50 um.
Mentions: Cross-sections of paraffin-embedded SCCs after 1 day of cultivation showed that the MSCs were uniformly distributed throughout the thickness of the scaffolds (Figure 10A). The images from the middle and from the right and left ends of the SCCs prove that a pronounced integration of cells occurred in the central portion of the SCCs. In the middle of the scaffolds, a larger number of cells could be visualized, while in the outermost portions, lesser number of cells was found. However, after 15 days of cultivation, the cross-sections of the SCCs demonstrated an increase in the number of cells and an improved filling of the scaffold structure by the MSCs (Figure 10B). The cells were able to proliferate and fill the ends of the SSCs. No difference was observed between the different portions of the scaffolds.

Bottom Line: Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds.SCCs exhibited good mechanical properties, compatible with their handling and further implantation.The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Hematology and Stem Cells Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil ; Department of Materials Science, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.

ABSTRACT
In tissue engineering, a uniform cell occupation of scaffolds is crucial to ensure the success of tissue regeneration. However, this point remains an unsolved problem in 3D scaffolds. In this study, a direct method to integrate cells into fiber scaffolds was investigated by combining the methods of electrospinning of fibers and bioelectrospraying of cells. With the associating of these methods, the cells were incorporated into the 3D scaffolds while the fibers were being produced. The scaffolds containing cells (SCCs) were produced using 20% poly(lactide-co-glycolide) solution for electrospinning and mesenchymal stem cells from deciduous teeth as a suspension for bioelectrospraying. After their production, the SCCs were cultivated for 15 days at 37°C with an atmosphere of 5% CO2. The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide test demonstrated that the cells remained viable and were able to grow between the fibers. Scanning electron microscopy showed the presence of a high number of cells in the structure of the scaffolds and confocal images demonstrated that the cells were able to adapt and spread between the fibers. Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds. Some physicochemical properties of the scaffolds were also investigated. SCCs exhibited good mechanical properties, compatible with their handling and further implantation. The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

No MeSH data available.