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Surface Plasmon Resonance Biosensor Based on Smart Phone Platforms.

Liu Y, Liu Q, Chen S, Cheng F, Wang H, Peng W - Sci Rep (2015)

Bottom Line: Utilizing a smart application to extract the light intensity information from the camera images, the light intensities of each channel are recorded every 0.5 s with refractive index (RI) changes.The performance of the smart phone-based SPR platform for accurate and repeatable measurements was evaluated by detecting different concentrations of antibody binding to a functionalized sensing element, and the experiment results were validated through contrast experiments with a commercial SPR instrument.This cost-effective and portable SPR biosensor based on smart phones has many applications, such as medicine, health and environmental monitoring.

View Article: PubMed Central - PubMed

Affiliation: School of Physics and Optoelectronic Engineering, Dalian University of Technology, Dalian 116024, China.

ABSTRACT
We demonstrate a fiber optic surface plasmon resonance (SPR) biosensor based on smart phone platforms. The light-weight optical components and sensing element are connected by optical fibers on a phone case. This SPR adaptor can be conveniently installed or removed from smart phones. The measurement, control and reference channels are illuminated by the light entering the lead-in fibers from the phone's LED flash, while the light from the end faces of the lead-out fibers is detected by the phone's camera. The SPR-sensing element is fabricated by a light-guiding silica capillary that is stripped off its cladding and coated with 50-nm gold film. Utilizing a smart application to extract the light intensity information from the camera images, the light intensities of each channel are recorded every 0.5 s with refractive index (RI) changes. The performance of the smart phone-based SPR platform for accurate and repeatable measurements was evaluated by detecting different concentrations of antibody binding to a functionalized sensing element, and the experiment results were validated through contrast experiments with a commercial SPR instrument. This cost-effective and portable SPR biosensor based on smart phones has many applications, such as medicine, health and environmental monitoring.

No MeSH data available.


Related in: MedlinePlus

Transmission spectrum of the capillary SPR sensor.(a) Spectral response of the capillary SPR sensor for solutions with RIs of 1.328 and 1.338. (b) FWHM of the capillary and HPOF SPR sensors.
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f5: Transmission spectrum of the capillary SPR sensor.(a) Spectral response of the capillary SPR sensor for solutions with RIs of 1.328 and 1.338. (b) FWHM of the capillary and HPOF SPR sensors.

Mentions: The operating principle of the smart phone SPR system relies on the penetration of the filter by the light from the smart phone flash and transmission to the lead-in fibers (Fig. 5). The 5-mm coating and cladding of the capillary was removed, and a gold film of 50 nm was coated onto the sensing region. Hence, when light reaches the sensing region of the capillary, some propagation modes enter resonance with the gold film due to the SPR effect. Because the SPR absorption depends on the dielectric properties of the thin layer of solution near the surface of the sensor region, we can monitor and analyze the binding interactions of the sample with SPR sensing. Thus, after calibration, the SPR device can measure samples quantitatively.


Surface Plasmon Resonance Biosensor Based on Smart Phone Platforms.

Liu Y, Liu Q, Chen S, Cheng F, Wang H, Peng W - Sci Rep (2015)

Transmission spectrum of the capillary SPR sensor.(a) Spectral response of the capillary SPR sensor for solutions with RIs of 1.328 and 1.338. (b) FWHM of the capillary and HPOF SPR sensors.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542615&req=5

f5: Transmission spectrum of the capillary SPR sensor.(a) Spectral response of the capillary SPR sensor for solutions with RIs of 1.328 and 1.338. (b) FWHM of the capillary and HPOF SPR sensors.
Mentions: The operating principle of the smart phone SPR system relies on the penetration of the filter by the light from the smart phone flash and transmission to the lead-in fibers (Fig. 5). The 5-mm coating and cladding of the capillary was removed, and a gold film of 50 nm was coated onto the sensing region. Hence, when light reaches the sensing region of the capillary, some propagation modes enter resonance with the gold film due to the SPR effect. Because the SPR absorption depends on the dielectric properties of the thin layer of solution near the surface of the sensor region, we can monitor and analyze the binding interactions of the sample with SPR sensing. Thus, after calibration, the SPR device can measure samples quantitatively.

Bottom Line: Utilizing a smart application to extract the light intensity information from the camera images, the light intensities of each channel are recorded every 0.5 s with refractive index (RI) changes.The performance of the smart phone-based SPR platform for accurate and repeatable measurements was evaluated by detecting different concentrations of antibody binding to a functionalized sensing element, and the experiment results were validated through contrast experiments with a commercial SPR instrument.This cost-effective and portable SPR biosensor based on smart phones has many applications, such as medicine, health and environmental monitoring.

View Article: PubMed Central - PubMed

Affiliation: School of Physics and Optoelectronic Engineering, Dalian University of Technology, Dalian 116024, China.

ABSTRACT
We demonstrate a fiber optic surface plasmon resonance (SPR) biosensor based on smart phone platforms. The light-weight optical components and sensing element are connected by optical fibers on a phone case. This SPR adaptor can be conveniently installed or removed from smart phones. The measurement, control and reference channels are illuminated by the light entering the lead-in fibers from the phone's LED flash, while the light from the end faces of the lead-out fibers is detected by the phone's camera. The SPR-sensing element is fabricated by a light-guiding silica capillary that is stripped off its cladding and coated with 50-nm gold film. Utilizing a smart application to extract the light intensity information from the camera images, the light intensities of each channel are recorded every 0.5 s with refractive index (RI) changes. The performance of the smart phone-based SPR platform for accurate and repeatable measurements was evaluated by detecting different concentrations of antibody binding to a functionalized sensing element, and the experiment results were validated through contrast experiments with a commercial SPR instrument. This cost-effective and portable SPR biosensor based on smart phones has many applications, such as medicine, health and environmental monitoring.

No MeSH data available.


Related in: MedlinePlus