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Surface Plasmon Resonance Biosensor Based on Smart Phone Platforms.

Liu Y, Liu Q, Chen S, Cheng F, Wang H, Peng W - Sci Rep (2015)

Bottom Line: Utilizing a smart application to extract the light intensity information from the camera images, the light intensities of each channel are recorded every 0.5 s with refractive index (RI) changes.The performance of the smart phone-based SPR platform for accurate and repeatable measurements was evaluated by detecting different concentrations of antibody binding to a functionalized sensing element, and the experiment results were validated through contrast experiments with a commercial SPR instrument.This cost-effective and portable SPR biosensor based on smart phones has many applications, such as medicine, health and environmental monitoring.

View Article: PubMed Central - PubMed

Affiliation: School of Physics and Optoelectronic Engineering, Dalian University of Technology, Dalian 116024, China.

ABSTRACT
We demonstrate a fiber optic surface plasmon resonance (SPR) biosensor based on smart phone platforms. The light-weight optical components and sensing element are connected by optical fibers on a phone case. This SPR adaptor can be conveniently installed or removed from smart phones. The measurement, control and reference channels are illuminated by the light entering the lead-in fibers from the phone's LED flash, while the light from the end faces of the lead-out fibers is detected by the phone's camera. The SPR-sensing element is fabricated by a light-guiding silica capillary that is stripped off its cladding and coated with 50-nm gold film. Utilizing a smart application to extract the light intensity information from the camera images, the light intensities of each channel are recorded every 0.5 s with refractive index (RI) changes. The performance of the smart phone-based SPR platform for accurate and repeatable measurements was evaluated by detecting different concentrations of antibody binding to a functionalized sensing element, and the experiment results were validated through contrast experiments with a commercial SPR instrument. This cost-effective and portable SPR biosensor based on smart phones has many applications, such as medicine, health and environmental monitoring.

No MeSH data available.


RI testing results for the prototype smart phone-based SPR imaging sensor.(a) Time response of the SPR sensor for solutions with different RIs. (b) Steady-state SPR response vs. refractive index units (RIU), from which the sensitivity and resolution were calculated.
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f2: RI testing results for the prototype smart phone-based SPR imaging sensor.(a) Time response of the SPR sensor for solutions with different RIs. (b) Steady-state SPR response vs. refractive index units (RIU), from which the sensitivity and resolution were calculated.

Mentions: Using a mini pump, the sensitivity and resolution of the SPR device were characterized by sodium chloride solutions with RIs of 1.328, 1.333, 1.338, 1.345, and 1.351 delivered into the flow cell at a constant flow rate of 180 μL/min (Fig. 2). The RIs of the sodium chloride solutions (calibration samples) were calibrated using an Abbe refractometer (WAY-2S). After fitting, the relative intensity had a linear response to the RI in the RI range of 1.328–1.351 (Fig. 2(b)), consistent with the range for most common biofluids. In the RI range of 1.328–1.363, the system provided a sensitivity of 1136%/RIU and a resolution greater than 7.4 × 10−5 RIU; the noise level is 0.08% and signal-noise ratio of the camera are better than 48 dB.


Surface Plasmon Resonance Biosensor Based on Smart Phone Platforms.

Liu Y, Liu Q, Chen S, Cheng F, Wang H, Peng W - Sci Rep (2015)

RI testing results for the prototype smart phone-based SPR imaging sensor.(a) Time response of the SPR sensor for solutions with different RIs. (b) Steady-state SPR response vs. refractive index units (RIU), from which the sensitivity and resolution were calculated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542615&req=5

f2: RI testing results for the prototype smart phone-based SPR imaging sensor.(a) Time response of the SPR sensor for solutions with different RIs. (b) Steady-state SPR response vs. refractive index units (RIU), from which the sensitivity and resolution were calculated.
Mentions: Using a mini pump, the sensitivity and resolution of the SPR device were characterized by sodium chloride solutions with RIs of 1.328, 1.333, 1.338, 1.345, and 1.351 delivered into the flow cell at a constant flow rate of 180 μL/min (Fig. 2). The RIs of the sodium chloride solutions (calibration samples) were calibrated using an Abbe refractometer (WAY-2S). After fitting, the relative intensity had a linear response to the RI in the RI range of 1.328–1.351 (Fig. 2(b)), consistent with the range for most common biofluids. In the RI range of 1.328–1.363, the system provided a sensitivity of 1136%/RIU and a resolution greater than 7.4 × 10−5 RIU; the noise level is 0.08% and signal-noise ratio of the camera are better than 48 dB.

Bottom Line: Utilizing a smart application to extract the light intensity information from the camera images, the light intensities of each channel are recorded every 0.5 s with refractive index (RI) changes.The performance of the smart phone-based SPR platform for accurate and repeatable measurements was evaluated by detecting different concentrations of antibody binding to a functionalized sensing element, and the experiment results were validated through contrast experiments with a commercial SPR instrument.This cost-effective and portable SPR biosensor based on smart phones has many applications, such as medicine, health and environmental monitoring.

View Article: PubMed Central - PubMed

Affiliation: School of Physics and Optoelectronic Engineering, Dalian University of Technology, Dalian 116024, China.

ABSTRACT
We demonstrate a fiber optic surface plasmon resonance (SPR) biosensor based on smart phone platforms. The light-weight optical components and sensing element are connected by optical fibers on a phone case. This SPR adaptor can be conveniently installed or removed from smart phones. The measurement, control and reference channels are illuminated by the light entering the lead-in fibers from the phone's LED flash, while the light from the end faces of the lead-out fibers is detected by the phone's camera. The SPR-sensing element is fabricated by a light-guiding silica capillary that is stripped off its cladding and coated with 50-nm gold film. Utilizing a smart application to extract the light intensity information from the camera images, the light intensities of each channel are recorded every 0.5 s with refractive index (RI) changes. The performance of the smart phone-based SPR platform for accurate and repeatable measurements was evaluated by detecting different concentrations of antibody binding to a functionalized sensing element, and the experiment results were validated through contrast experiments with a commercial SPR instrument. This cost-effective and portable SPR biosensor based on smart phones has many applications, such as medicine, health and environmental monitoring.

No MeSH data available.