The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria.
Bottom Line: The rulAB operon of Pseudomonas spp. confers fitness traits on the host and has been suggested to be a hotspot for insertion of mobile elements that carry avirulence genes.Integration into rulB on pWW0 occurred at position 6488 generating a 3 bp direct repeat.Downstream of these resided ORFs of the 'variable side' with structural and sequence similarity to those encoding survival traits on the fitness enhancing plasmid pGRT1 (ILE(FH1) and ILE(FH5)) and the NR-II virulence region of genomic island PAGI-5 (ILE(FH4)).
Affiliation: Centre for Ecology and Hydrology, Lancaster Environment Centre, Library Avenue, Bailrigg, Lancaster, LA1 4AP, UK.Show MeSH
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Mentions: The sequence of the ILE on pWW0::ILEFH1 ascertained from pFBA1001 elucidated the structure and location on pWW0 but did not confirm its location or structure in the genome of strain FH1. To better understand this, we obtained the draft genome sequences of strains FH1, FH4 and FH5, which based on RFLP profile, data represented three different ILEs. The ILEs within strains FH1, FH4 and FH5 were located in the draft sequences by alignment using the ILE sequences inserted into rulB on pWW0 in each strain. Interestingly, in the case of all three strains, ILEs were located inside a chromosomal rulB gene within a disrupted rulAB-like operon that differed to rulAB on pWW0 (see Fig. 3).
Affiliation: Centre for Ecology and Hydrology, Lancaster Environment Centre, Library Avenue, Bailrigg, Lancaster, LA1 4AP, UK.