The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria.
Bottom Line: Here, for the first time, we show that rulB on plasmid pWW0 is a hotspot for the active site-specific integration of related integron-like elements (ILEs) found in six environmental pseudomonads (strains FH1-FH6).Integration into rulB on pWW0 occurred at position 6488 generating a 3 bp direct repeat.Collectively, these ILEs share features with the previously described type III protein secretion system effector ILEs and are considered important to host survival and transfer of fitness enhancing and (a)virulence genes between bacteria.
Affiliation: Centre for Ecology and Hydrology, Lancaster Environment Centre, Library Avenue, Bailrigg, Lancaster, LA1 4AP, UK.Show MeSH
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Mentions: The sequence of the ILE on pWW0::ILEFH1 ascertained from pFBA1001 elucidated the structure and location on pWW0 but did not confirm its location or structure in the genome of strain FH1. To better understand this, we obtained the draft genome sequences of strains FH1, FH4 and FH5, which based on RFLP profile, data represented three different ILEs. The ILEs within strains FH1, FH4 and FH5 were located in the draft sequences by alignment using the ILE sequences inserted into rulB on pWW0 in each strain. Interestingly, in the case of all three strains, ILEs were located inside a chromosomal rulB gene within a disrupted rulAB-like operon that differed to rulAB on pWW0 (see Fig. 3).
Affiliation: Centre for Ecology and Hydrology, Lancaster Environment Centre, Library Avenue, Bailrigg, Lancaster, LA1 4AP, UK.