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Biogeography of Nocardiopsis strains from hypersaline environments of Yunnan and Xinjiang Provinces, western China.

He ST, Zhi XY, Jiang H, Yang LL, Wu JY, Zhang YG, Hozzein WN, Li WJ - Sci Rep (2015)

Bottom Line: Such information is essential for understanding the ecology of Nocardiopsis.Here we analyzed 16S rRNA, gyrB, rpoB and sodA genes of 78 Nocardiopsis strains isolated from hypersaline environments in Yunnan and Xinjiang Provinces of western China.Statistical analyses showed that spatial distance and environmental factors substantially influenced Nocardiopsis distribution in hypersaline environments: the former had stronger influence at large spatial scales, whereas the latter was more influential at small spatial scales.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, China.

ABSTRACT
The genus Nocardiopsis is a widespread group within the phylum Actinobacteria and has been isolated from various salty environments worldwide. However, little is known about whether biogeography affects Nocardiopsis distribution in various hypersaline environments. Such information is essential for understanding the ecology of Nocardiopsis. Here we analyzed 16S rRNA, gyrB, rpoB and sodA genes of 78 Nocardiopsis strains isolated from hypersaline environments in Yunnan and Xinjiang Provinces of western China. The obtained Nocardiopsis strains were classified into five operational taxonomic units, each comprising location-specific phylo- and genotypes. Statistical analyses showed that spatial distance and environmental factors substantially influenced Nocardiopsis distribution in hypersaline environments: the former had stronger influence at large spatial scales, whereas the latter was more influential at small spatial scales.

No MeSH data available.


Canonical correlation analysis of linear relationships between geochemistry variations, and differentiations of endemic genotypes among the eight sampling sites (A–C) panels are for gyrB, rpoB, and sodA genes, respectively). In Nocardiopsis xinjiangensis, inverse solid (▼) and open (▽) triangles and upright solid triangles (▲) denote NXX, NXY and NDX phylotypes/genotypes from Xinjiang sites, respectively; upright open triangles denote NDY phylotypes/genotypes from Yunnan sites; in Nocardiopsis quinghaiensis, solid (●) and open (◯) circles denote NQX and NQY phylotypes/genotypes from Xinjiang and Yunnan sites, respectively; in Nocardiopsis aegyptia, Solid (■) and open (□) squares denote NAX and NAY phylotypes/genotypes from Xinjiang and Yunnan sites, respectively; in Nocardiopsis terrae, solid (◆) and open (◇) diamonds denote NTX and NTY phylotypes/genotypes from Xinjiang and Yunnan sites, respectively.
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f4: Canonical correlation analysis of linear relationships between geochemistry variations, and differentiations of endemic genotypes among the eight sampling sites (A–C) panels are for gyrB, rpoB, and sodA genes, respectively). In Nocardiopsis xinjiangensis, inverse solid (▼) and open (▽) triangles and upright solid triangles (▲) denote NXX, NXY and NDX phylotypes/genotypes from Xinjiang sites, respectively; upright open triangles denote NDY phylotypes/genotypes from Yunnan sites; in Nocardiopsis quinghaiensis, solid (●) and open (◯) circles denote NQX and NQY phylotypes/genotypes from Xinjiang and Yunnan sites, respectively; in Nocardiopsis aegyptia, Solid (■) and open (□) squares denote NAX and NAY phylotypes/genotypes from Xinjiang and Yunnan sites, respectively; in Nocardiopsis terrae, solid (◆) and open (◇) diamonds denote NTX and NTY phylotypes/genotypes from Xinjiang and Yunnan sites, respectively.

Mentions: MLST of four housekeeping genes revealed significant correlations of gene sequences with geographic distance (16S rRNA: r = 0.83, p = 0.005; gyrB: r = 0.81, p = 0.005; rpoB: r = 0.87, p = 0.003; sodA: r = 0.89, p =0.002) (Fig. 3). In addition, the closely related phylo- or genotypes were present within a very small scale (<100 km) but not at distant locations (>100 km) (Fig. 3). Mantel test (r > 0.5, P < 0.05) and canonical correlation analysis showed that the differentiations of endemic genotypes of gyrB, rpoB and sodA genes were significantly correlated with the geochemistry variations of sediments from eight habitats between Yunnan and Xinjiang Provinces (Table 2; Fig. 4).


Biogeography of Nocardiopsis strains from hypersaline environments of Yunnan and Xinjiang Provinces, western China.

He ST, Zhi XY, Jiang H, Yang LL, Wu JY, Zhang YG, Hozzein WN, Li WJ - Sci Rep (2015)

Canonical correlation analysis of linear relationships between geochemistry variations, and differentiations of endemic genotypes among the eight sampling sites (A–C) panels are for gyrB, rpoB, and sodA genes, respectively). In Nocardiopsis xinjiangensis, inverse solid (▼) and open (▽) triangles and upright solid triangles (▲) denote NXX, NXY and NDX phylotypes/genotypes from Xinjiang sites, respectively; upright open triangles denote NDY phylotypes/genotypes from Yunnan sites; in Nocardiopsis quinghaiensis, solid (●) and open (◯) circles denote NQX and NQY phylotypes/genotypes from Xinjiang and Yunnan sites, respectively; in Nocardiopsis aegyptia, Solid (■) and open (□) squares denote NAX and NAY phylotypes/genotypes from Xinjiang and Yunnan sites, respectively; in Nocardiopsis terrae, solid (◆) and open (◇) diamonds denote NTX and NTY phylotypes/genotypes from Xinjiang and Yunnan sites, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542603&req=5

f4: Canonical correlation analysis of linear relationships between geochemistry variations, and differentiations of endemic genotypes among the eight sampling sites (A–C) panels are for gyrB, rpoB, and sodA genes, respectively). In Nocardiopsis xinjiangensis, inverse solid (▼) and open (▽) triangles and upright solid triangles (▲) denote NXX, NXY and NDX phylotypes/genotypes from Xinjiang sites, respectively; upright open triangles denote NDY phylotypes/genotypes from Yunnan sites; in Nocardiopsis quinghaiensis, solid (●) and open (◯) circles denote NQX and NQY phylotypes/genotypes from Xinjiang and Yunnan sites, respectively; in Nocardiopsis aegyptia, Solid (■) and open (□) squares denote NAX and NAY phylotypes/genotypes from Xinjiang and Yunnan sites, respectively; in Nocardiopsis terrae, solid (◆) and open (◇) diamonds denote NTX and NTY phylotypes/genotypes from Xinjiang and Yunnan sites, respectively.
Mentions: MLST of four housekeeping genes revealed significant correlations of gene sequences with geographic distance (16S rRNA: r = 0.83, p = 0.005; gyrB: r = 0.81, p = 0.005; rpoB: r = 0.87, p = 0.003; sodA: r = 0.89, p =0.002) (Fig. 3). In addition, the closely related phylo- or genotypes were present within a very small scale (<100 km) but not at distant locations (>100 km) (Fig. 3). Mantel test (r > 0.5, P < 0.05) and canonical correlation analysis showed that the differentiations of endemic genotypes of gyrB, rpoB and sodA genes were significantly correlated with the geochemistry variations of sediments from eight habitats between Yunnan and Xinjiang Provinces (Table 2; Fig. 4).

Bottom Line: Such information is essential for understanding the ecology of Nocardiopsis.Here we analyzed 16S rRNA, gyrB, rpoB and sodA genes of 78 Nocardiopsis strains isolated from hypersaline environments in Yunnan and Xinjiang Provinces of western China.Statistical analyses showed that spatial distance and environmental factors substantially influenced Nocardiopsis distribution in hypersaline environments: the former had stronger influence at large spatial scales, whereas the latter was more influential at small spatial scales.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, China.

ABSTRACT
The genus Nocardiopsis is a widespread group within the phylum Actinobacteria and has been isolated from various salty environments worldwide. However, little is known about whether biogeography affects Nocardiopsis distribution in various hypersaline environments. Such information is essential for understanding the ecology of Nocardiopsis. Here we analyzed 16S rRNA, gyrB, rpoB and sodA genes of 78 Nocardiopsis strains isolated from hypersaline environments in Yunnan and Xinjiang Provinces of western China. The obtained Nocardiopsis strains were classified into five operational taxonomic units, each comprising location-specific phylo- and genotypes. Statistical analyses showed that spatial distance and environmental factors substantially influenced Nocardiopsis distribution in hypersaline environments: the former had stronger influence at large spatial scales, whereas the latter was more influential at small spatial scales.

No MeSH data available.