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Role of Neurexin-1β and Neuroligin-1 in Cognitive Dysfunction After Subarachnoid Hemorrhage in Rats.

Shen H, Chen Z, Wang Y, Gao A, Li H, Cui Y, Zhang L, Xu X, Wang Z, Chen G - Stroke (2015)

Bottom Line: Both in vivo and in vitro experiments showed SAH-induced decrease in the expressions of neurexin-1β and neuroligin-1 and the interaction between neurexin-1β and neuroligin-1 in neurons.In addition, the interaction between neurexin-1β and neuroligin-1 was reduced by their knockdown and increased by their overexpression.The formation of excitatory synapses was inhibited by oxyhemoglobin treatment, which was significantly ameliorated by overexpression of neurexin-1β and neuroligin-1 and aggravated by the knockdown of neurexin-1β and neuroligin-1.

View Article: PubMed Central - PubMed

Affiliation: From the Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China (H.S., Z.C., Y.W., A.G., H.L., Y.C., L.Z., X.X., Z.W., G.C.); and Department of Neurosurgery, Anhui Provincial Hospital Affiliated to Anhui Medical University, Anhui Province Key Laboratory of Brain Function and Brain Disease, Hefei, Anhui, China (Y.W.).

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Effects of oxyhemoglobin (OxyHb) on the expressions of neurexin-1β and neuroligin-1, and the interaction between them in cultured hippocampal neurons under OxyHb treatment. A, Western blot (WB) analysis of the protein levels of neurexin-1β and neuroligin-1 in cultured primary hippocampal neurons exposed to OxyHb. B, Quantification of relative protein levels of neurexin-1β and neuroligin-1. Data are presented as mean±SEM. *P<0.05 compared with control (Ctr) group, n=3. C, Immunoprecipitation analysis of the effect of OxyHb on the interaction between neurexin-1β and neuroligin-1 in cultured primary hippocampal neurons exposed to OxyHb. D, Quantification of relative protein levels of neurexin-1β and neuroligin-1. Values are mean±SEM. **P<0.01 compared with control (Ctr) group, n=6.
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Figure 3: Effects of oxyhemoglobin (OxyHb) on the expressions of neurexin-1β and neuroligin-1, and the interaction between them in cultured hippocampal neurons under OxyHb treatment. A, Western blot (WB) analysis of the protein levels of neurexin-1β and neuroligin-1 in cultured primary hippocampal neurons exposed to OxyHb. B, Quantification of relative protein levels of neurexin-1β and neuroligin-1. Data are presented as mean±SEM. *P<0.05 compared with control (Ctr) group, n=3. C, Immunoprecipitation analysis of the effect of OxyHb on the interaction between neurexin-1β and neuroligin-1 in cultured primary hippocampal neurons exposed to OxyHb. D, Quantification of relative protein levels of neurexin-1β and neuroligin-1. Values are mean±SEM. **P<0.01 compared with control (Ctr) group, n=6.

Mentions: Consistent with the in vivo data, Western blot and immunoprecipitation assay showed that both the protein levels of neurexin-1β and neuroligin-1 and the interaction between them were significantly decreased by oxyhemoglobin treatment (Figure 3).


Role of Neurexin-1β and Neuroligin-1 in Cognitive Dysfunction After Subarachnoid Hemorrhage in Rats.

Shen H, Chen Z, Wang Y, Gao A, Li H, Cui Y, Zhang L, Xu X, Wang Z, Chen G - Stroke (2015)

Effects of oxyhemoglobin (OxyHb) on the expressions of neurexin-1β and neuroligin-1, and the interaction between them in cultured hippocampal neurons under OxyHb treatment. A, Western blot (WB) analysis of the protein levels of neurexin-1β and neuroligin-1 in cultured primary hippocampal neurons exposed to OxyHb. B, Quantification of relative protein levels of neurexin-1β and neuroligin-1. Data are presented as mean±SEM. *P<0.05 compared with control (Ctr) group, n=3. C, Immunoprecipitation analysis of the effect of OxyHb on the interaction between neurexin-1β and neuroligin-1 in cultured primary hippocampal neurons exposed to OxyHb. D, Quantification of relative protein levels of neurexin-1β and neuroligin-1. Values are mean±SEM. **P<0.01 compared with control (Ctr) group, n=6.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4542569&req=5

Figure 3: Effects of oxyhemoglobin (OxyHb) on the expressions of neurexin-1β and neuroligin-1, and the interaction between them in cultured hippocampal neurons under OxyHb treatment. A, Western blot (WB) analysis of the protein levels of neurexin-1β and neuroligin-1 in cultured primary hippocampal neurons exposed to OxyHb. B, Quantification of relative protein levels of neurexin-1β and neuroligin-1. Data are presented as mean±SEM. *P<0.05 compared with control (Ctr) group, n=3. C, Immunoprecipitation analysis of the effect of OxyHb on the interaction between neurexin-1β and neuroligin-1 in cultured primary hippocampal neurons exposed to OxyHb. D, Quantification of relative protein levels of neurexin-1β and neuroligin-1. Values are mean±SEM. **P<0.01 compared with control (Ctr) group, n=6.
Mentions: Consistent with the in vivo data, Western blot and immunoprecipitation assay showed that both the protein levels of neurexin-1β and neuroligin-1 and the interaction between them were significantly decreased by oxyhemoglobin treatment (Figure 3).

Bottom Line: Both in vivo and in vitro experiments showed SAH-induced decrease in the expressions of neurexin-1β and neuroligin-1 and the interaction between neurexin-1β and neuroligin-1 in neurons.In addition, the interaction between neurexin-1β and neuroligin-1 was reduced by their knockdown and increased by their overexpression.The formation of excitatory synapses was inhibited by oxyhemoglobin treatment, which was significantly ameliorated by overexpression of neurexin-1β and neuroligin-1 and aggravated by the knockdown of neurexin-1β and neuroligin-1.

View Article: PubMed Central - PubMed

Affiliation: From the Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China (H.S., Z.C., Y.W., A.G., H.L., Y.C., L.Z., X.X., Z.W., G.C.); and Department of Neurosurgery, Anhui Provincial Hospital Affiliated to Anhui Medical University, Anhui Province Key Laboratory of Brain Function and Brain Disease, Hefei, Anhui, China (Y.W.).

Show MeSH
Related in: MedlinePlus