Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke.
Bottom Line: Hematoxylin and eosin staining revealed significant 15% and 30% reductions in striatal infarct and peri-infarct area, and a trend of rescue against neuronal loss in the hippocampus.Human antigen immunostaining revealed 0.03% hBMSCs survived in spleen and only 0.0007% in brain.MSC migration to spleen, but not brain, inversely correlated with reduced infarct, peri-infarct, and inflammation. hBMSC transplantation is therapeutic in chronic stroke possibly by abrogating the inflammation-plagued secondary cell death.
Affiliation: From the Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa.Show MeSH
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Mentions: ANOVA revealed significant treatment effects on infarct area (F2,12=25.66, P<0.001) and peri-infarct area (F2,12=23.27, P<0.001; Figure 3A and 3B). Post hoc test revealed significant infarct and peri-infarct area in the ipsilateral (P<0.001), but not the contralateral (P>0.05) striatum of vehicle-infused stroke animals compared with sham animals In addition, there were significant infarct and peri-infarct areas in the ipsilateral (P<0.001), but not the contralateral (P>0.05) striatum of hBMSC-transplanted animals relative to sham animals (P<0.001). Interestingly, there were significant reductions of 15% and 30% in infarct and peri-infarct in the ipsilateral striatum, respectively, of hBMSC-transplanted stroke animals relative to vehicle-infused stroke animals (P<0.05; Figure 3A and 3B). There were no significant differences between the contralateral striatum of hBMSC-transplanted stroke animals and vehicle-infused stroke animals.
Affiliation: From the Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa.