Limits...
Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke.

Acosta SA, Tajiri N, Hoover J, Kaneko Y, Borlongan CV - Stroke (2015)

Bottom Line: Hematoxylin and eosin staining revealed significant 15% and 30% reductions in striatal infarct and peri-infarct area, and a trend of rescue against neuronal loss in the hippocampus.Human antigen immunostaining revealed 0.03% hBMSCs survived in spleen and only 0.0007% in brain.MSC migration to spleen, but not brain, inversely correlated with reduced infarct, peri-infarct, and inflammation. hBMSC transplantation is therapeutic in chronic stroke possibly by abrogating the inflammation-plagued secondary cell death.

View Article: PubMed Central - PubMed

Affiliation: From the Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa.

Show MeSH

Related in: MedlinePlus

Homing and anti-inflammatory effects of bone marrow stromal cells (hBMSC) transplantation reduces infarct and peri-infarct area, but does not ameliorate hippocampal CA1 and CA3 neuronal loss. Hematoxylin and eosin (H&E) staining revealed that hBMSC treatment significantly reduced the striatal infarct and peri-infarct areas associated with stroke. A, Quantitative analysis revealed a significant reductions in infarct and peri-infarct area after hBMSC transplantation compared with vehicle-infused stroke animals (*P<0.05). B, Photomicrographs are representative coronal brain sections stained with H&E at 11 days post transplantation, showing infarct area (dark purple) and intact areas (light purple). Arrows denote infarct and peri-infarct area of striatum. Right inserts, vehicle-infused stroke exhibited acidic cells (dark purple cells), shrinkage, and dissolution of the cells in the infarcted striatum and peri-infarct area compared with the corresponding ipsilateral side of hBMSC-transplanted stroke animals. Scale bar: 1 mm. C and D, Quantitative analyses of total number of CA1 and CA3 neurons failed to reveal a significant reduction in neuronal cell loss in rats that received hBMSCs compared with vehicle-infused stroke rats (*P>0.05). C and D, Photomicrographs are representative coronal brain sections staining with H&E from ipsilateral CA1 and CA3 area of the hippocampus of sham, vehicle-infused, and hBMSC-treated stroke animals. Arrows denote neuronal cell loss within the CA1 and CA3 region. Scale bar=50 μm. *P<0.05; ns=not significant. Data are expressed as percentage difference from contralateral CA1 and CA3 total number of neurons.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4542567&req=5

Figure 3: Homing and anti-inflammatory effects of bone marrow stromal cells (hBMSC) transplantation reduces infarct and peri-infarct area, but does not ameliorate hippocampal CA1 and CA3 neuronal loss. Hematoxylin and eosin (H&E) staining revealed that hBMSC treatment significantly reduced the striatal infarct and peri-infarct areas associated with stroke. A, Quantitative analysis revealed a significant reductions in infarct and peri-infarct area after hBMSC transplantation compared with vehicle-infused stroke animals (*P<0.05). B, Photomicrographs are representative coronal brain sections stained with H&E at 11 days post transplantation, showing infarct area (dark purple) and intact areas (light purple). Arrows denote infarct and peri-infarct area of striatum. Right inserts, vehicle-infused stroke exhibited acidic cells (dark purple cells), shrinkage, and dissolution of the cells in the infarcted striatum and peri-infarct area compared with the corresponding ipsilateral side of hBMSC-transplanted stroke animals. Scale bar: 1 mm. C and D, Quantitative analyses of total number of CA1 and CA3 neurons failed to reveal a significant reduction in neuronal cell loss in rats that received hBMSCs compared with vehicle-infused stroke rats (*P>0.05). C and D, Photomicrographs are representative coronal brain sections staining with H&E from ipsilateral CA1 and CA3 area of the hippocampus of sham, vehicle-infused, and hBMSC-treated stroke animals. Arrows denote neuronal cell loss within the CA1 and CA3 region. Scale bar=50 μm. *P<0.05; ns=not significant. Data are expressed as percentage difference from contralateral CA1 and CA3 total number of neurons.

Mentions: ANOVA revealed significant treatment effects on infarct area (F2,12=25.66, P<0.001) and peri-infarct area (F2,12=23.27, P<0.001; Figure 3A and 3B). Post hoc test revealed significant infarct and peri-infarct area in the ipsilateral (P<0.001), but not the contralateral (P>0.05) striatum of vehicle-infused stroke animals compared with sham animals In addition, there were significant infarct and peri-infarct areas in the ipsilateral (P<0.001), but not the contralateral (P>0.05) striatum of hBMSC-transplanted animals relative to sham animals (P<0.001). Interestingly, there were significant reductions of 15% and 30% in infarct and peri-infarct in the ipsilateral striatum, respectively, of hBMSC-transplanted stroke animals relative to vehicle-infused stroke animals (P<0.05; Figure 3A and 3B). There were no significant differences between the contralateral striatum of hBMSC-transplanted stroke animals and vehicle-infused stroke animals.


Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke.

Acosta SA, Tajiri N, Hoover J, Kaneko Y, Borlongan CV - Stroke (2015)

Homing and anti-inflammatory effects of bone marrow stromal cells (hBMSC) transplantation reduces infarct and peri-infarct area, but does not ameliorate hippocampal CA1 and CA3 neuronal loss. Hematoxylin and eosin (H&E) staining revealed that hBMSC treatment significantly reduced the striatal infarct and peri-infarct areas associated with stroke. A, Quantitative analysis revealed a significant reductions in infarct and peri-infarct area after hBMSC transplantation compared with vehicle-infused stroke animals (*P<0.05). B, Photomicrographs are representative coronal brain sections stained with H&E at 11 days post transplantation, showing infarct area (dark purple) and intact areas (light purple). Arrows denote infarct and peri-infarct area of striatum. Right inserts, vehicle-infused stroke exhibited acidic cells (dark purple cells), shrinkage, and dissolution of the cells in the infarcted striatum and peri-infarct area compared with the corresponding ipsilateral side of hBMSC-transplanted stroke animals. Scale bar: 1 mm. C and D, Quantitative analyses of total number of CA1 and CA3 neurons failed to reveal a significant reduction in neuronal cell loss in rats that received hBMSCs compared with vehicle-infused stroke rats (*P>0.05). C and D, Photomicrographs are representative coronal brain sections staining with H&E from ipsilateral CA1 and CA3 area of the hippocampus of sham, vehicle-infused, and hBMSC-treated stroke animals. Arrows denote neuronal cell loss within the CA1 and CA3 region. Scale bar=50 μm. *P<0.05; ns=not significant. Data are expressed as percentage difference from contralateral CA1 and CA3 total number of neurons.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4542567&req=5

Figure 3: Homing and anti-inflammatory effects of bone marrow stromal cells (hBMSC) transplantation reduces infarct and peri-infarct area, but does not ameliorate hippocampal CA1 and CA3 neuronal loss. Hematoxylin and eosin (H&E) staining revealed that hBMSC treatment significantly reduced the striatal infarct and peri-infarct areas associated with stroke. A, Quantitative analysis revealed a significant reductions in infarct and peri-infarct area after hBMSC transplantation compared with vehicle-infused stroke animals (*P<0.05). B, Photomicrographs are representative coronal brain sections stained with H&E at 11 days post transplantation, showing infarct area (dark purple) and intact areas (light purple). Arrows denote infarct and peri-infarct area of striatum. Right inserts, vehicle-infused stroke exhibited acidic cells (dark purple cells), shrinkage, and dissolution of the cells in the infarcted striatum and peri-infarct area compared with the corresponding ipsilateral side of hBMSC-transplanted stroke animals. Scale bar: 1 mm. C and D, Quantitative analyses of total number of CA1 and CA3 neurons failed to reveal a significant reduction in neuronal cell loss in rats that received hBMSCs compared with vehicle-infused stroke rats (*P>0.05). C and D, Photomicrographs are representative coronal brain sections staining with H&E from ipsilateral CA1 and CA3 area of the hippocampus of sham, vehicle-infused, and hBMSC-treated stroke animals. Arrows denote neuronal cell loss within the CA1 and CA3 region. Scale bar=50 μm. *P<0.05; ns=not significant. Data are expressed as percentage difference from contralateral CA1 and CA3 total number of neurons.
Mentions: ANOVA revealed significant treatment effects on infarct area (F2,12=25.66, P<0.001) and peri-infarct area (F2,12=23.27, P<0.001; Figure 3A and 3B). Post hoc test revealed significant infarct and peri-infarct area in the ipsilateral (P<0.001), but not the contralateral (P>0.05) striatum of vehicle-infused stroke animals compared with sham animals In addition, there were significant infarct and peri-infarct areas in the ipsilateral (P<0.001), but not the contralateral (P>0.05) striatum of hBMSC-transplanted animals relative to sham animals (P<0.001). Interestingly, there were significant reductions of 15% and 30% in infarct and peri-infarct in the ipsilateral striatum, respectively, of hBMSC-transplanted stroke animals relative to vehicle-infused stroke animals (P<0.05; Figure 3A and 3B). There were no significant differences between the contralateral striatum of hBMSC-transplanted stroke animals and vehicle-infused stroke animals.

Bottom Line: Hematoxylin and eosin staining revealed significant 15% and 30% reductions in striatal infarct and peri-infarct area, and a trend of rescue against neuronal loss in the hippocampus.Human antigen immunostaining revealed 0.03% hBMSCs survived in spleen and only 0.0007% in brain.MSC migration to spleen, but not brain, inversely correlated with reduced infarct, peri-infarct, and inflammation. hBMSC transplantation is therapeutic in chronic stroke possibly by abrogating the inflammation-plagued secondary cell death.

View Article: PubMed Central - PubMed

Affiliation: From the Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa.

Show MeSH
Related in: MedlinePlus