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Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke.

Acosta SA, Tajiri N, Hoover J, Kaneko Y, Borlongan CV - Stroke (2015)

Bottom Line: Hematoxylin and eosin staining revealed significant 15% and 30% reductions in striatal infarct and peri-infarct area, and a trend of rescue against neuronal loss in the hippocampus.Human antigen immunostaining revealed 0.03% hBMSCs survived in spleen and only 0.0007% in brain.MSC migration to spleen, but not brain, inversely correlated with reduced infarct, peri-infarct, and inflammation. hBMSC transplantation is therapeutic in chronic stroke possibly by abrogating the inflammation-plagued secondary cell death.

View Article: PubMed Central - PubMed

Affiliation: From the Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa.

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Preferential ex vivo migration of hBMSC-DiR+ to spleen in chronic stroke.A, Quantitative analysis of fluorescent signals brain, spleen, lungs, and liver confirmed preferential migration of hBMSC-DiR+ to the spleen over the brain (*P<0.05). B, Photographs correspond to representative peripheral organs ex vivo. B, Sham animals (brain, spleen, lungs, and liver) and (C) hBMSC-DiR+ (brain, spleen, lungs, and liver). Radiant efficiency={(p/sec/cm2/sr)/(μW/cm2)}, color scale: min=3.74×107; max=4.99×107. *P<0.05, **P<0.01, and ***P<0.001. Data are expressed as mean±SEM. DiR indicates 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricbocyanine iodide; and hBMSC, homing and anti-inflammatory effects of bone marrow stromal cells.
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Figure 2: Preferential ex vivo migration of hBMSC-DiR+ to spleen in chronic stroke.A, Quantitative analysis of fluorescent signals brain, spleen, lungs, and liver confirmed preferential migration of hBMSC-DiR+ to the spleen over the brain (*P<0.05). B, Photographs correspond to representative peripheral organs ex vivo. B, Sham animals (brain, spleen, lungs, and liver) and (C) hBMSC-DiR+ (brain, spleen, lungs, and liver). Radiant efficiency={(p/sec/cm2/sr)/(μW/cm2)}, color scale: min=3.74×107; max=4.99×107. *P<0.05, **P<0.01, and ***P<0.001. Data are expressed as mean±SEM. DiR indicates 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricbocyanine iodide; and hBMSC, homing and anti-inflammatory effects of bone marrow stromal cells.

Mentions: ANOVA revealed that there was a significant treatment effect based on fluorescent signals detected in peripheral organs and brain ex vivo (F3,11=15.25, P<0.001; Figure 2A–2C). Pairwise comparisons using post hoc tests revealed that the brain emitted a significantly lower fluorescent signal relative to the spleen of rats exposed to chronic stroke (P<0.05). The liver and lung also emitted significantly higher signals than the brain, but the spleen displayed the most prominent fluorescent signals among the peripheral organs (P<0.05).


Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke.

Acosta SA, Tajiri N, Hoover J, Kaneko Y, Borlongan CV - Stroke (2015)

Preferential ex vivo migration of hBMSC-DiR+ to spleen in chronic stroke.A, Quantitative analysis of fluorescent signals brain, spleen, lungs, and liver confirmed preferential migration of hBMSC-DiR+ to the spleen over the brain (*P<0.05). B, Photographs correspond to representative peripheral organs ex vivo. B, Sham animals (brain, spleen, lungs, and liver) and (C) hBMSC-DiR+ (brain, spleen, lungs, and liver). Radiant efficiency={(p/sec/cm2/sr)/(μW/cm2)}, color scale: min=3.74×107; max=4.99×107. *P<0.05, **P<0.01, and ***P<0.001. Data are expressed as mean±SEM. DiR indicates 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricbocyanine iodide; and hBMSC, homing and anti-inflammatory effects of bone marrow stromal cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4542567&req=5

Figure 2: Preferential ex vivo migration of hBMSC-DiR+ to spleen in chronic stroke.A, Quantitative analysis of fluorescent signals brain, spleen, lungs, and liver confirmed preferential migration of hBMSC-DiR+ to the spleen over the brain (*P<0.05). B, Photographs correspond to representative peripheral organs ex vivo. B, Sham animals (brain, spleen, lungs, and liver) and (C) hBMSC-DiR+ (brain, spleen, lungs, and liver). Radiant efficiency={(p/sec/cm2/sr)/(μW/cm2)}, color scale: min=3.74×107; max=4.99×107. *P<0.05, **P<0.01, and ***P<0.001. Data are expressed as mean±SEM. DiR indicates 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricbocyanine iodide; and hBMSC, homing and anti-inflammatory effects of bone marrow stromal cells.
Mentions: ANOVA revealed that there was a significant treatment effect based on fluorescent signals detected in peripheral organs and brain ex vivo (F3,11=15.25, P<0.001; Figure 2A–2C). Pairwise comparisons using post hoc tests revealed that the brain emitted a significantly lower fluorescent signal relative to the spleen of rats exposed to chronic stroke (P<0.05). The liver and lung also emitted significantly higher signals than the brain, but the spleen displayed the most prominent fluorescent signals among the peripheral organs (P<0.05).

Bottom Line: Hematoxylin and eosin staining revealed significant 15% and 30% reductions in striatal infarct and peri-infarct area, and a trend of rescue against neuronal loss in the hippocampus.Human antigen immunostaining revealed 0.03% hBMSCs survived in spleen and only 0.0007% in brain.MSC migration to spleen, but not brain, inversely correlated with reduced infarct, peri-infarct, and inflammation. hBMSC transplantation is therapeutic in chronic stroke possibly by abrogating the inflammation-plagued secondary cell death.

View Article: PubMed Central - PubMed

Affiliation: From the Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa.

Show MeSH
Related in: MedlinePlus