Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke.
Bottom Line: Hematoxylin and eosin staining revealed significant 15% and 30% reductions in striatal infarct and peri-infarct area, and a trend of rescue against neuronal loss in the hippocampus.Human antigen immunostaining revealed 0.03% hBMSCs survived in spleen and only 0.0007% in brain.MSC migration to spleen, but not brain, inversely correlated with reduced infarct, peri-infarct, and inflammation. hBMSC transplantation is therapeutic in chronic stroke possibly by abrogating the inflammation-plagued secondary cell death.
Affiliation: From the Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa.Show MeSH
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Mentions: ANOVA revealed that there was a significant treatment effects based on fluorescent signals detected in the spleen (F3,11=4.953, P<0.001; Figure 1A and 1B). Pairwise comparison using post hoc tests revealed that hBMSC-DiR+ transplanted cells migrated to the spleen, emitting significantly higher fluorescent signals across all time points compared with hBMSC-DiR− transplanted and vehicle-infused stroke animals, and sham animals (P<0.05). Moreover, within the hBMSC-DiR+ transplanted stroke group, there were no significant differences in fluorescent signals across all time points except the first hour, whereby a higher emitted signal was found within spleen and on day 11 where the signal was greatly reduced relative to other time points (P<0.05). The signal that we detected from the transplanted group was specific for the DiR labeling because hBMSC-DiR− transplanted and vehicle-infused stroke animals, and sham animals, which were similarly subjected to the imaging assay displayed no detectable fluorescent signal (Figure 1A and 1B).
Affiliation: From the Center of Excellence for Aging and Brain Repair, Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa.