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Enhancement of radiotherapy by ceria nanoparticles modified with neogambogic acid in breast cancer cells.

Chen F, Zhang XH, Hu XD, Zhang W, Lou ZC, Xie LH, Liu PD, Zhang HQ - Int J Nanomedicine (2015)

Bottom Line: NGA-CNPs potentiated the toxic effects of radiation, leading to a higher rate of cell death than either treatment used alone and inducing the activation of autophagy and cell cycle arrest at the G2/M phase, while pretreatment with NGA or CNPs did not improve the rate of radiation-induced cancer cells death.However, NGA-CNPs decreased both endogenous and radiation-induced reactive oxygen species formation, unlike other nanomaterials.These results suggest that the adjunctive use of NGA-CNPs can increase the effectiveness of radiotherapy in breast cancer treatment by lowering the radiation doses required to kill cancer cells and thereby minimizing collateral damage to healthy adjacent tissue.

View Article: PubMed Central - PubMed

Affiliation: College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing, People's Republic of China.

ABSTRACT
Radiotherapy is one of the main strategies for cancer treatment but has significant challenges, such as cancer cell resistance and radiation damage to normal tissue. Radiosensitizers that selectively increase the susceptibility of cancer cells to radiation can enhance the effectiveness of radiotherapy. We report here the development of a novel radiosensitizer consisting of monodispersed ceria nanoparticles (CNPs) covered with the anticancer drug neogambogic acid (NGA-CNPs). These were used in conjunction with radiation in MCF-7 breast cancer cells, and the efficacy and mechanisms of action of this combined treatment approach were evaluated. NGA-CNPs potentiated the toxic effects of radiation, leading to a higher rate of cell death than either treatment used alone and inducing the activation of autophagy and cell cycle arrest at the G2/M phase, while pretreatment with NGA or CNPs did not improve the rate of radiation-induced cancer cells death. However, NGA-CNPs decreased both endogenous and radiation-induced reactive oxygen species formation, unlike other nanomaterials. These results suggest that the adjunctive use of NGA-CNPs can increase the effectiveness of radiotherapy in breast cancer treatment by lowering the radiation doses required to kill cancer cells and thereby minimizing collateral damage to healthy adjacent tissue.

No MeSH data available.


Related in: MedlinePlus

Enhancement of radiation-induced growth inhibition by NGA, CNP, and NGA-CNPs.Notes: The surviving fraction of MCF-7 cells was decreased by RT in a dose-dependent manner, an effect that was potentiated by the addition of NGA-CNPs. (C), compared with NGA (A), or CNPs (B).Abbreviations: NGA, neogambogic acid; CNP, ceria nanoparticle; NGA-CNP, ceria nanoparticle modified with neogambogic acid; RT, irradiation.
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f4-ijn-10-4957: Enhancement of radiation-induced growth inhibition by NGA, CNP, and NGA-CNPs.Notes: The surviving fraction of MCF-7 cells was decreased by RT in a dose-dependent manner, an effect that was potentiated by the addition of NGA-CNPs. (C), compared with NGA (A), or CNPs (B).Abbreviations: NGA, neogambogic acid; CNP, ceria nanoparticle; NGA-CNP, ceria nanoparticle modified with neogambogic acid; RT, irradiation.

Mentions: Previous studies have documented the anticancer activities of CNPs and NGA in various tumor models.20,29 To determine whether NGA, CNPs, or NGA-CNPs increase cellular sensitivity to radiation, breast cancer cell growth upon exposure to both was evaluated with the clonogenic assay. MCF-7 cells were exposed to three different concentrations of NGA (0 μg/mL, 0.5 μg/mL, or 1 μg/mL), CNP (0 μg/mL, 10 μg/mL, or 20 μg/mL), or NGA-CNP (0 μg/mL, 10 μg/mL, or 20 μg/mL) for 24 hours prior to irradiation at one of five doses (0 Gy, 2 Gy, 4 Gy, 6 Gy, or 8 Gy). The doses of NGA were the same as those used for the synthesis of NGA-CNP. Exposure to 4 Gy radiation decreased the colony formation rate to 49.03%. However, at 0.5 μg/mL and 1 μg/mL NGA, colony formation was reduced to 37.01% and 34.89%, respectively (P<0.05 vs 4 Gy radiation only); at 10 μg/mL and 20 μg/mL CNP, colony formation was reduced to 46.32% and 35.27%, respectively; and at 10 μg/mL and 20 μg/mL NGA-CNP, colony formation was reduced to 40.69% and 31.97%, respectively (P<0.05 vs 4 Gy radiation only) (Figure 4). The inhibitory effects of other doses of radiation on colony formation were likewise enhanced by combined treatment with NGA-CNPs, which reduced the colony-forming efficiency of cancer cells to a greater extent than NGA or CNP, suggesting that NGA-CNPs sensitize cells to the effects of radiation.


Enhancement of radiotherapy by ceria nanoparticles modified with neogambogic acid in breast cancer cells.

Chen F, Zhang XH, Hu XD, Zhang W, Lou ZC, Xie LH, Liu PD, Zhang HQ - Int J Nanomedicine (2015)

Enhancement of radiation-induced growth inhibition by NGA, CNP, and NGA-CNPs.Notes: The surviving fraction of MCF-7 cells was decreased by RT in a dose-dependent manner, an effect that was potentiated by the addition of NGA-CNPs. (C), compared with NGA (A), or CNPs (B).Abbreviations: NGA, neogambogic acid; CNP, ceria nanoparticle; NGA-CNP, ceria nanoparticle modified with neogambogic acid; RT, irradiation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542556&req=5

f4-ijn-10-4957: Enhancement of radiation-induced growth inhibition by NGA, CNP, and NGA-CNPs.Notes: The surviving fraction of MCF-7 cells was decreased by RT in a dose-dependent manner, an effect that was potentiated by the addition of NGA-CNPs. (C), compared with NGA (A), or CNPs (B).Abbreviations: NGA, neogambogic acid; CNP, ceria nanoparticle; NGA-CNP, ceria nanoparticle modified with neogambogic acid; RT, irradiation.
Mentions: Previous studies have documented the anticancer activities of CNPs and NGA in various tumor models.20,29 To determine whether NGA, CNPs, or NGA-CNPs increase cellular sensitivity to radiation, breast cancer cell growth upon exposure to both was evaluated with the clonogenic assay. MCF-7 cells were exposed to three different concentrations of NGA (0 μg/mL, 0.5 μg/mL, or 1 μg/mL), CNP (0 μg/mL, 10 μg/mL, or 20 μg/mL), or NGA-CNP (0 μg/mL, 10 μg/mL, or 20 μg/mL) for 24 hours prior to irradiation at one of five doses (0 Gy, 2 Gy, 4 Gy, 6 Gy, or 8 Gy). The doses of NGA were the same as those used for the synthesis of NGA-CNP. Exposure to 4 Gy radiation decreased the colony formation rate to 49.03%. However, at 0.5 μg/mL and 1 μg/mL NGA, colony formation was reduced to 37.01% and 34.89%, respectively (P<0.05 vs 4 Gy radiation only); at 10 μg/mL and 20 μg/mL CNP, colony formation was reduced to 46.32% and 35.27%, respectively; and at 10 μg/mL and 20 μg/mL NGA-CNP, colony formation was reduced to 40.69% and 31.97%, respectively (P<0.05 vs 4 Gy radiation only) (Figure 4). The inhibitory effects of other doses of radiation on colony formation were likewise enhanced by combined treatment with NGA-CNPs, which reduced the colony-forming efficiency of cancer cells to a greater extent than NGA or CNP, suggesting that NGA-CNPs sensitize cells to the effects of radiation.

Bottom Line: NGA-CNPs potentiated the toxic effects of radiation, leading to a higher rate of cell death than either treatment used alone and inducing the activation of autophagy and cell cycle arrest at the G2/M phase, while pretreatment with NGA or CNPs did not improve the rate of radiation-induced cancer cells death.However, NGA-CNPs decreased both endogenous and radiation-induced reactive oxygen species formation, unlike other nanomaterials.These results suggest that the adjunctive use of NGA-CNPs can increase the effectiveness of radiotherapy in breast cancer treatment by lowering the radiation doses required to kill cancer cells and thereby minimizing collateral damage to healthy adjacent tissue.

View Article: PubMed Central - PubMed

Affiliation: College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing, People's Republic of China.

ABSTRACT
Radiotherapy is one of the main strategies for cancer treatment but has significant challenges, such as cancer cell resistance and radiation damage to normal tissue. Radiosensitizers that selectively increase the susceptibility of cancer cells to radiation can enhance the effectiveness of radiotherapy. We report here the development of a novel radiosensitizer consisting of monodispersed ceria nanoparticles (CNPs) covered with the anticancer drug neogambogic acid (NGA-CNPs). These were used in conjunction with radiation in MCF-7 breast cancer cells, and the efficacy and mechanisms of action of this combined treatment approach were evaluated. NGA-CNPs potentiated the toxic effects of radiation, leading to a higher rate of cell death than either treatment used alone and inducing the activation of autophagy and cell cycle arrest at the G2/M phase, while pretreatment with NGA or CNPs did not improve the rate of radiation-induced cancer cells death. However, NGA-CNPs decreased both endogenous and radiation-induced reactive oxygen species formation, unlike other nanomaterials. These results suggest that the adjunctive use of NGA-CNPs can increase the effectiveness of radiotherapy in breast cancer treatment by lowering the radiation doses required to kill cancer cells and thereby minimizing collateral damage to healthy adjacent tissue.

No MeSH data available.


Related in: MedlinePlus