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Enhancement of radiotherapy by ceria nanoparticles modified with neogambogic acid in breast cancer cells.

Chen F, Zhang XH, Hu XD, Zhang W, Lou ZC, Xie LH, Liu PD, Zhang HQ - Int J Nanomedicine (2015)

Bottom Line: NGA-CNPs potentiated the toxic effects of radiation, leading to a higher rate of cell death than either treatment used alone and inducing the activation of autophagy and cell cycle arrest at the G2/M phase, while pretreatment with NGA or CNPs did not improve the rate of radiation-induced cancer cells death.However, NGA-CNPs decreased both endogenous and radiation-induced reactive oxygen species formation, unlike other nanomaterials.These results suggest that the adjunctive use of NGA-CNPs can increase the effectiveness of radiotherapy in breast cancer treatment by lowering the radiation doses required to kill cancer cells and thereby minimizing collateral damage to healthy adjacent tissue.

View Article: PubMed Central - PubMed

Affiliation: College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing, People's Republic of China.

ABSTRACT
Radiotherapy is one of the main strategies for cancer treatment but has significant challenges, such as cancer cell resistance and radiation damage to normal tissue. Radiosensitizers that selectively increase the susceptibility of cancer cells to radiation can enhance the effectiveness of radiotherapy. We report here the development of a novel radiosensitizer consisting of monodispersed ceria nanoparticles (CNPs) covered with the anticancer drug neogambogic acid (NGA-CNPs). These were used in conjunction with radiation in MCF-7 breast cancer cells, and the efficacy and mechanisms of action of this combined treatment approach were evaluated. NGA-CNPs potentiated the toxic effects of radiation, leading to a higher rate of cell death than either treatment used alone and inducing the activation of autophagy and cell cycle arrest at the G2/M phase, while pretreatment with NGA or CNPs did not improve the rate of radiation-induced cancer cells death. However, NGA-CNPs decreased both endogenous and radiation-induced reactive oxygen species formation, unlike other nanomaterials. These results suggest that the adjunctive use of NGA-CNPs can increase the effectiveness of radiotherapy in breast cancer treatment by lowering the radiation doses required to kill cancer cells and thereby minimizing collateral damage to healthy adjacent tissue.

No MeSH data available.


Related in: MedlinePlus

Structural analysis of CNPs.Notes: (A) Particle size distribution (3–5 nm) of CNPs as determined by TE microscopy. The selected-area electron diffraction pattern (inset) revealed the crystallinity and fluorite structure of CNPs. (B) X-ray diffraction pattern of CNPs; 111, 200, 220, and 311 correspond to the different lattice planes of the CNP crystal structure.Abbreviations: CNP, ceria nanoparticle; TE, transmission electron.
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f1-ijn-10-4957: Structural analysis of CNPs.Notes: (A) Particle size distribution (3–5 nm) of CNPs as determined by TE microscopy. The selected-area electron diffraction pattern (inset) revealed the crystallinity and fluorite structure of CNPs. (B) X-ray diffraction pattern of CNPs; 111, 200, 220, and 311 correspond to the different lattice planes of the CNP crystal structure.Abbreviations: CNP, ceria nanoparticle; TE, transmission electron.

Mentions: The size of CNPs synthesized by the microemulsion method ranged between 3 nm and 5 nm (Figure 1A). The selected-area electron diffraction pattern, which indicates the crystallinity and fluorite structure of the NPs, showed lattice planes at 111, 200, 220, and 311. The TE microscopy results were supported by the X-ray diffraction pattern (Figure 1B).


Enhancement of radiotherapy by ceria nanoparticles modified with neogambogic acid in breast cancer cells.

Chen F, Zhang XH, Hu XD, Zhang W, Lou ZC, Xie LH, Liu PD, Zhang HQ - Int J Nanomedicine (2015)

Structural analysis of CNPs.Notes: (A) Particle size distribution (3–5 nm) of CNPs as determined by TE microscopy. The selected-area electron diffraction pattern (inset) revealed the crystallinity and fluorite structure of CNPs. (B) X-ray diffraction pattern of CNPs; 111, 200, 220, and 311 correspond to the different lattice planes of the CNP crystal structure.Abbreviations: CNP, ceria nanoparticle; TE, transmission electron.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542556&req=5

f1-ijn-10-4957: Structural analysis of CNPs.Notes: (A) Particle size distribution (3–5 nm) of CNPs as determined by TE microscopy. The selected-area electron diffraction pattern (inset) revealed the crystallinity and fluorite structure of CNPs. (B) X-ray diffraction pattern of CNPs; 111, 200, 220, and 311 correspond to the different lattice planes of the CNP crystal structure.Abbreviations: CNP, ceria nanoparticle; TE, transmission electron.
Mentions: The size of CNPs synthesized by the microemulsion method ranged between 3 nm and 5 nm (Figure 1A). The selected-area electron diffraction pattern, which indicates the crystallinity and fluorite structure of the NPs, showed lattice planes at 111, 200, 220, and 311. The TE microscopy results were supported by the X-ray diffraction pattern (Figure 1B).

Bottom Line: NGA-CNPs potentiated the toxic effects of radiation, leading to a higher rate of cell death than either treatment used alone and inducing the activation of autophagy and cell cycle arrest at the G2/M phase, while pretreatment with NGA or CNPs did not improve the rate of radiation-induced cancer cells death.However, NGA-CNPs decreased both endogenous and radiation-induced reactive oxygen species formation, unlike other nanomaterials.These results suggest that the adjunctive use of NGA-CNPs can increase the effectiveness of radiotherapy in breast cancer treatment by lowering the radiation doses required to kill cancer cells and thereby minimizing collateral damage to healthy adjacent tissue.

View Article: PubMed Central - PubMed

Affiliation: College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing, People's Republic of China.

ABSTRACT
Radiotherapy is one of the main strategies for cancer treatment but has significant challenges, such as cancer cell resistance and radiation damage to normal tissue. Radiosensitizers that selectively increase the susceptibility of cancer cells to radiation can enhance the effectiveness of radiotherapy. We report here the development of a novel radiosensitizer consisting of monodispersed ceria nanoparticles (CNPs) covered with the anticancer drug neogambogic acid (NGA-CNPs). These were used in conjunction with radiation in MCF-7 breast cancer cells, and the efficacy and mechanisms of action of this combined treatment approach were evaluated. NGA-CNPs potentiated the toxic effects of radiation, leading to a higher rate of cell death than either treatment used alone and inducing the activation of autophagy and cell cycle arrest at the G2/M phase, while pretreatment with NGA or CNPs did not improve the rate of radiation-induced cancer cells death. However, NGA-CNPs decreased both endogenous and radiation-induced reactive oxygen species formation, unlike other nanomaterials. These results suggest that the adjunctive use of NGA-CNPs can increase the effectiveness of radiotherapy in breast cancer treatment by lowering the radiation doses required to kill cancer cells and thereby minimizing collateral damage to healthy adjacent tissue.

No MeSH data available.


Related in: MedlinePlus