Limits...
Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy.

Huang HL, Lu PH, Yang HC, Lee GD, Li HR, Liao KC - Int J Nanomedicine (2015)

Bottom Line: The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes.In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements.Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan.

ABSTRACT
The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes embedded with gold nanoparticles (AuNPs) using a 200 μm fiber with 65 mW and 532 nm excitation for topical release in vivo. The tunable delivery function can be paired with an apoptosis biosensor based on the same fiber-optic configuration for providing real-time evaluation of chemotherapy efficacy in vivo to perform as a personalized chemotherapy system. The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes. In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements. Using the mouse xenograft studies, we first demonstrated that the encapsulation of fluorescein in liposomes resulted in a more substantial content retention (81%) in the tumor than for free fluorophores (14%) at 120 minutes after administration from in vivo fluorescence imaging. Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

No MeSH data available.


Related in: MedlinePlus

Profile of triggered liposome release under consecutive pulsatile excitation.Notes: (A) Laser excitation durations. (B) Consecutive release in vitro (rat plasma). (C) Consecutive release in vivo (mice xenograft).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4542555&req=5

f9-ijn-10-5171: Profile of triggered liposome release under consecutive pulsatile excitation.Notes: (A) Laser excitation durations. (B) Consecutive release in vitro (rat plasma). (C) Consecutive release in vivo (mice xenograft).

Mentions: Upon consecutive fiber-optic guided laser excitation of 75 mM liposome-encapsulated fluorescein in vivo, a significant (72%, [normalized fluorescence at 60th minute – normalized fluorescence at 50th minute]/normalized fluorescence at 50th minute) fluorescence increase was observed at the tumor area immediately after the first 10 minutes (50th–60th minutes, Figure 9A) of laser excitation, while a relatively milder fluorescence increase (33%, [normalized fluorescence at 120th minute–normalized fluorescence at 110th minute]/normalized fluorescence at 110th minute) was observed after the second 10 minutes (110th–120th minutes, Figure 9A) of laser excitation (Figure 9C), which was consistent with accumulation fluorescein quantity upon consecutive fiber-optic guided laser excitation of 75 mM liposome-encapsulated fluorescein in vitro (rat plasma) as shown in Figure 9B.


Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy.

Huang HL, Lu PH, Yang HC, Lee GD, Li HR, Liao KC - Int J Nanomedicine (2015)

Profile of triggered liposome release under consecutive pulsatile excitation.Notes: (A) Laser excitation durations. (B) Consecutive release in vitro (rat plasma). (C) Consecutive release in vivo (mice xenograft).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542555&req=5

f9-ijn-10-5171: Profile of triggered liposome release under consecutive pulsatile excitation.Notes: (A) Laser excitation durations. (B) Consecutive release in vitro (rat plasma). (C) Consecutive release in vivo (mice xenograft).
Mentions: Upon consecutive fiber-optic guided laser excitation of 75 mM liposome-encapsulated fluorescein in vivo, a significant (72%, [normalized fluorescence at 60th minute – normalized fluorescence at 50th minute]/normalized fluorescence at 50th minute) fluorescence increase was observed at the tumor area immediately after the first 10 minutes (50th–60th minutes, Figure 9A) of laser excitation, while a relatively milder fluorescence increase (33%, [normalized fluorescence at 120th minute–normalized fluorescence at 110th minute]/normalized fluorescence at 110th minute) was observed after the second 10 minutes (110th–120th minutes, Figure 9A) of laser excitation (Figure 9C), which was consistent with accumulation fluorescein quantity upon consecutive fiber-optic guided laser excitation of 75 mM liposome-encapsulated fluorescein in vitro (rat plasma) as shown in Figure 9B.

Bottom Line: The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes.In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements.Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan.

ABSTRACT
The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes embedded with gold nanoparticles (AuNPs) using a 200 μm fiber with 65 mW and 532 nm excitation for topical release in vivo. The tunable delivery function can be paired with an apoptosis biosensor based on the same fiber-optic configuration for providing real-time evaluation of chemotherapy efficacy in vivo to perform as a personalized chemotherapy system. The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes. In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements. Using the mouse xenograft studies, we first demonstrated that the encapsulation of fluorescein in liposomes resulted in a more substantial content retention (81%) in the tumor than for free fluorophores (14%) at 120 minutes after administration from in vivo fluorescence imaging. Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

No MeSH data available.


Related in: MedlinePlus