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Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy.

Huang HL, Lu PH, Yang HC, Lee GD, Li HR, Liao KC - Int J Nanomedicine (2015)

Bottom Line: The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes.In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements.Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan.

ABSTRACT
The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes embedded with gold nanoparticles (AuNPs) using a 200 μm fiber with 65 mW and 532 nm excitation for topical release in vivo. The tunable delivery function can be paired with an apoptosis biosensor based on the same fiber-optic configuration for providing real-time evaluation of chemotherapy efficacy in vivo to perform as a personalized chemotherapy system. The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes. In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements. Using the mouse xenograft studies, we first demonstrated that the encapsulation of fluorescein in liposomes resulted in a more substantial content retention (81%) in the tumor than for free fluorophores (14%) at 120 minutes after administration from in vivo fluorescence imaging. Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

No MeSH data available.


Related in: MedlinePlus

Fiber-optic guided laser excitation triggered photo-thermal responsive liposomes release in vivo.Notes: (A) Releasing profile of 75 mM fluorescein (in self-quench concentration) encapsulated in liposome with excitation. (B) Quantification of releasing profiles with or without excitation. The position of tumor in (A) is indicated by the white arrowhead in the bright-field (BF) images. Data of (B) represent mean ± standard deviation (n=5).Abbreviation: AuNP, gold nanoparticle.
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f8-ijn-10-5171: Fiber-optic guided laser excitation triggered photo-thermal responsive liposomes release in vivo.Notes: (A) Releasing profile of 75 mM fluorescein (in self-quench concentration) encapsulated in liposome with excitation. (B) Quantification of releasing profiles with or without excitation. The position of tumor in (A) is indicated by the white arrowhead in the bright-field (BF) images. Data of (B) represent mean ± standard deviation (n=5).Abbreviation: AuNP, gold nanoparticle.

Mentions: Fiber-optic guided laser excitation triggered liposome releases in vivo were investigated with the xenograft mice model mentioned above. After anesthesia, the mice received a 50 μl bolus injection of 75 mM fluorescein aqueous solution encapsulated in AuNP-liposomes. A 10-minute duration of CW laser excitation was selected for consecutive tunable release (with ~50% fluorescein remaining in the liposome after the first triggered release, see Figure 4D, red curve). The tumor boundary was delineated with specific fluorescence contour levels of pixels on bright field images (BF) of mice at times after 75 mM fluorescein in AuNp-liposome injection, such as 0.5 before laser excitation, and 1, 0.4, 0.4 at 0+, 60, 120 min (Figure 8A). The laser excited liposomes had a significant (+42%, [normalized fluorescence 0+ minute after excitation–normalized fluorescence before excitation]/normalized fluorescence before excitation) normalized fluorescence increase at the tumor area immediately after 10 minutes of excitation (Figure 8B, red curve), while liposome without excitation had gradually declined normalized fluorescence at the tumor area (Figure 8B, blue curve).


Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy.

Huang HL, Lu PH, Yang HC, Lee GD, Li HR, Liao KC - Int J Nanomedicine (2015)

Fiber-optic guided laser excitation triggered photo-thermal responsive liposomes release in vivo.Notes: (A) Releasing profile of 75 mM fluorescein (in self-quench concentration) encapsulated in liposome with excitation. (B) Quantification of releasing profiles with or without excitation. The position of tumor in (A) is indicated by the white arrowhead in the bright-field (BF) images. Data of (B) represent mean ± standard deviation (n=5).Abbreviation: AuNP, gold nanoparticle.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4542555&req=5

f8-ijn-10-5171: Fiber-optic guided laser excitation triggered photo-thermal responsive liposomes release in vivo.Notes: (A) Releasing profile of 75 mM fluorescein (in self-quench concentration) encapsulated in liposome with excitation. (B) Quantification of releasing profiles with or without excitation. The position of tumor in (A) is indicated by the white arrowhead in the bright-field (BF) images. Data of (B) represent mean ± standard deviation (n=5).Abbreviation: AuNP, gold nanoparticle.
Mentions: Fiber-optic guided laser excitation triggered liposome releases in vivo were investigated with the xenograft mice model mentioned above. After anesthesia, the mice received a 50 μl bolus injection of 75 mM fluorescein aqueous solution encapsulated in AuNP-liposomes. A 10-minute duration of CW laser excitation was selected for consecutive tunable release (with ~50% fluorescein remaining in the liposome after the first triggered release, see Figure 4D, red curve). The tumor boundary was delineated with specific fluorescence contour levels of pixels on bright field images (BF) of mice at times after 75 mM fluorescein in AuNp-liposome injection, such as 0.5 before laser excitation, and 1, 0.4, 0.4 at 0+, 60, 120 min (Figure 8A). The laser excited liposomes had a significant (+42%, [normalized fluorescence 0+ minute after excitation–normalized fluorescence before excitation]/normalized fluorescence before excitation) normalized fluorescence increase at the tumor area immediately after 10 minutes of excitation (Figure 8B, red curve), while liposome without excitation had gradually declined normalized fluorescence at the tumor area (Figure 8B, blue curve).

Bottom Line: The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes.In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements.Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan.

ABSTRACT
The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes embedded with gold nanoparticles (AuNPs) using a 200 μm fiber with 65 mW and 532 nm excitation for topical release in vivo. The tunable delivery function can be paired with an apoptosis biosensor based on the same fiber-optic configuration for providing real-time evaluation of chemotherapy efficacy in vivo to perform as a personalized chemotherapy system. The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes. In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements. Using the mouse xenograft studies, we first demonstrated that the encapsulation of fluorescein in liposomes resulted in a more substantial content retention (81%) in the tumor than for free fluorophores (14%) at 120 minutes after administration from in vivo fluorescence imaging. Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

No MeSH data available.


Related in: MedlinePlus