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Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy.

Huang HL, Lu PH, Yang HC, Lee GD, Li HR, Liao KC - Int J Nanomedicine (2015)

Bottom Line: The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes.In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements.Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan.

ABSTRACT
The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes embedded with gold nanoparticles (AuNPs) using a 200 μm fiber with 65 mW and 532 nm excitation for topical release in vivo. The tunable delivery function can be paired with an apoptosis biosensor based on the same fiber-optic configuration for providing real-time evaluation of chemotherapy efficacy in vivo to perform as a personalized chemotherapy system. The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes. In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements. Using the mouse xenograft studies, we first demonstrated that the encapsulation of fluorescein in liposomes resulted in a more substantial content retention (81%) in the tumor than for free fluorophores (14%) at 120 minutes after administration from in vivo fluorescence imaging. Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

No MeSH data available.


Related in: MedlinePlus

Characterization of synthesized gold nanoparticles.Notes: (A) Morphology observed by transmission electron microscopy (black bar =10 nm). (B) Particle size distribution detected by dynamic laser scattering. (C) Surface plasma resonance band identified by absorbance spectrum with a maximal absorbance at approximately 522 nm. (D) Face-centered crystal lattice indicated by X-ray diffraction spectrum.Abbreviation: au, arbitrary unit.
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f5-ijn-10-5171: Characterization of synthesized gold nanoparticles.Notes: (A) Morphology observed by transmission electron microscopy (black bar =10 nm). (B) Particle size distribution detected by dynamic laser scattering. (C) Surface plasma resonance band identified by absorbance spectrum with a maximal absorbance at approximately 522 nm. (D) Face-centered crystal lattice indicated by X-ray diffraction spectrum.Abbreviation: au, arbitrary unit.

Mentions: The absorbance spectrum of the resultant dark-reddish chloroform solution (Figure 3B) had a well-defined surface plasma resonance band with a maximal absorbance at approximately 522 nm (Figure 5C), confirming the existence of AuNPs in the chloroform solution. Those hydrophobic AuNPs were spherical in shape and had a uniform particle size distribution around 3–7 nm, as observed with HR-TEM (Figure 5A). Further examination by DLS analysis suggested that the particle size ranged from 2 nm to 11 nm, mainly distributed from 3 nm to 7 nm with an average value of 5.55±2.1 nm, and had size distribution polydiversity index smaller than 0.079 (Figure 5B). The crystal lattice of the nanostructure was in face-centered cubic shape, as identified by the X-ray diffraction pattern with several lattice planes (1 1 1), (2 0 0), (2 2 0), (3 1 1), and (2 2 2) as shown in Figure 5D.


Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy.

Huang HL, Lu PH, Yang HC, Lee GD, Li HR, Liao KC - Int J Nanomedicine (2015)

Characterization of synthesized gold nanoparticles.Notes: (A) Morphology observed by transmission electron microscopy (black bar =10 nm). (B) Particle size distribution detected by dynamic laser scattering. (C) Surface plasma resonance band identified by absorbance spectrum with a maximal absorbance at approximately 522 nm. (D) Face-centered crystal lattice indicated by X-ray diffraction spectrum.Abbreviation: au, arbitrary unit.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542555&req=5

f5-ijn-10-5171: Characterization of synthesized gold nanoparticles.Notes: (A) Morphology observed by transmission electron microscopy (black bar =10 nm). (B) Particle size distribution detected by dynamic laser scattering. (C) Surface plasma resonance band identified by absorbance spectrum with a maximal absorbance at approximately 522 nm. (D) Face-centered crystal lattice indicated by X-ray diffraction spectrum.Abbreviation: au, arbitrary unit.
Mentions: The absorbance spectrum of the resultant dark-reddish chloroform solution (Figure 3B) had a well-defined surface plasma resonance band with a maximal absorbance at approximately 522 nm (Figure 5C), confirming the existence of AuNPs in the chloroform solution. Those hydrophobic AuNPs were spherical in shape and had a uniform particle size distribution around 3–7 nm, as observed with HR-TEM (Figure 5A). Further examination by DLS analysis suggested that the particle size ranged from 2 nm to 11 nm, mainly distributed from 3 nm to 7 nm with an average value of 5.55±2.1 nm, and had size distribution polydiversity index smaller than 0.079 (Figure 5B). The crystal lattice of the nanostructure was in face-centered cubic shape, as identified by the X-ray diffraction pattern with several lattice planes (1 1 1), (2 0 0), (2 2 0), (3 1 1), and (2 2 2) as shown in Figure 5D.

Bottom Line: The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes.In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements.Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan.

ABSTRACT
The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes embedded with gold nanoparticles (AuNPs) using a 200 μm fiber with 65 mW and 532 nm excitation for topical release in vivo. The tunable delivery function can be paired with an apoptosis biosensor based on the same fiber-optic configuration for providing real-time evaluation of chemotherapy efficacy in vivo to perform as a personalized chemotherapy system. The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes. In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements. Using the mouse xenograft studies, we first demonstrated that the encapsulation of fluorescein in liposomes resulted in a more substantial content retention (81%) in the tumor than for free fluorophores (14%) at 120 minutes after administration from in vivo fluorescence imaging. Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

No MeSH data available.


Related in: MedlinePlus