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Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy.

Huang HL, Lu PH, Yang HC, Lee GD, Li HR, Liao KC - Int J Nanomedicine (2015)

Bottom Line: The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes.In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements.Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan.

ABSTRACT
The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes embedded with gold nanoparticles (AuNPs) using a 200 μm fiber with 65 mW and 532 nm excitation for topical release in vivo. The tunable delivery function can be paired with an apoptosis biosensor based on the same fiber-optic configuration for providing real-time evaluation of chemotherapy efficacy in vivo to perform as a personalized chemotherapy system. The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes. In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements. Using the mouse xenograft studies, we first demonstrated that the encapsulation of fluorescein in liposomes resulted in a more substantial content retention (81%) in the tumor than for free fluorophores (14%) at 120 minutes after administration from in vivo fluorescence imaging. Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

No MeSH data available.


Related in: MedlinePlus

Photo-thermal energy conversion by AuNPs in chloroform.Notes: (A) Illustration of apparatus. (B) Appearance of collimated laser beam exiting optical fiber and illuminating into dark-reddish AuNP solution in chloroform. (C) AuNPs concentration and excitation duration dependent photo-thermal conversion profiles with fixed light path (h=2 cm). Data of (C) represent mean ± standard deviation (n=3).Abbreviation: AuNP, gold nanoparticle.
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f3-ijn-10-5171: Photo-thermal energy conversion by AuNPs in chloroform.Notes: (A) Illustration of apparatus. (B) Appearance of collimated laser beam exiting optical fiber and illuminating into dark-reddish AuNP solution in chloroform. (C) AuNPs concentration and excitation duration dependent photo-thermal conversion profiles with fixed light path (h=2 cm). Data of (C) represent mean ± standard deviation (n=3).Abbreviation: AuNP, gold nanoparticle.

Mentions: In all, 2 mL AuNPs solution in chloroform at different concentrations was loaded in 15×125 mm pyrex test tubes with silicone seal. The solution in each test tube was wrapped with aluminum foil and stimulated with 200 μm multi-mode optical fiber (FIBER-200-VIS, OceanOptics, Dunedin, FL, USA) guided 65 mW diode pumped solid-state laser (TWC Opto Corp, Taipei, Taiwan) with the emission end of the optical fiber was placed beneath the surface of the solution through the silicone seal and 2 cm above the bottom of the test tube (Figure 3A). A digital thermometer (GE-363D, Dr AV Technology Co. Ltd., Changhua, Taiwan) was also placed beneath the surface of the solution through the silicone seal about the same depth of the end of optical fiber to monitor the excitation time-dependent temperature changes of the solution under continuously excitation.


Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy.

Huang HL, Lu PH, Yang HC, Lee GD, Li HR, Liao KC - Int J Nanomedicine (2015)

Photo-thermal energy conversion by AuNPs in chloroform.Notes: (A) Illustration of apparatus. (B) Appearance of collimated laser beam exiting optical fiber and illuminating into dark-reddish AuNP solution in chloroform. (C) AuNPs concentration and excitation duration dependent photo-thermal conversion profiles with fixed light path (h=2 cm). Data of (C) represent mean ± standard deviation (n=3).Abbreviation: AuNP, gold nanoparticle.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542555&req=5

f3-ijn-10-5171: Photo-thermal energy conversion by AuNPs in chloroform.Notes: (A) Illustration of apparatus. (B) Appearance of collimated laser beam exiting optical fiber and illuminating into dark-reddish AuNP solution in chloroform. (C) AuNPs concentration and excitation duration dependent photo-thermal conversion profiles with fixed light path (h=2 cm). Data of (C) represent mean ± standard deviation (n=3).Abbreviation: AuNP, gold nanoparticle.
Mentions: In all, 2 mL AuNPs solution in chloroform at different concentrations was loaded in 15×125 mm pyrex test tubes with silicone seal. The solution in each test tube was wrapped with aluminum foil and stimulated with 200 μm multi-mode optical fiber (FIBER-200-VIS, OceanOptics, Dunedin, FL, USA) guided 65 mW diode pumped solid-state laser (TWC Opto Corp, Taipei, Taiwan) with the emission end of the optical fiber was placed beneath the surface of the solution through the silicone seal and 2 cm above the bottom of the test tube (Figure 3A). A digital thermometer (GE-363D, Dr AV Technology Co. Ltd., Changhua, Taiwan) was also placed beneath the surface of the solution through the silicone seal about the same depth of the end of optical fiber to monitor the excitation time-dependent temperature changes of the solution under continuously excitation.

Bottom Line: The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes.In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements.Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan.

ABSTRACT
The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes embedded with gold nanoparticles (AuNPs) using a 200 μm fiber with 65 mW and 532 nm excitation for topical release in vivo. The tunable delivery function can be paired with an apoptosis biosensor based on the same fiber-optic configuration for providing real-time evaluation of chemotherapy efficacy in vivo to perform as a personalized chemotherapy system. The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes. In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements. Using the mouse xenograft studies, we first demonstrated that the encapsulation of fluorescein in liposomes resulted in a more substantial content retention (81%) in the tumor than for free fluorophores (14%) at 120 minutes after administration from in vivo fluorescence imaging. Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

No MeSH data available.


Related in: MedlinePlus