Limits...
Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy.

Huang HL, Lu PH, Yang HC, Lee GD, Li HR, Liao KC - Int J Nanomedicine (2015)

Bottom Line: The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes.In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements.Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan.

ABSTRACT
The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes embedded with gold nanoparticles (AuNPs) using a 200 μm fiber with 65 mW and 532 nm excitation for topical release in vivo. The tunable delivery function can be paired with an apoptosis biosensor based on the same fiber-optic configuration for providing real-time evaluation of chemotherapy efficacy in vivo to perform as a personalized chemotherapy system. The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes. In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements. Using the mouse xenograft studies, we first demonstrated that the encapsulation of fluorescein in liposomes resulted in a more substantial content retention (81%) in the tumor than for free fluorophores (14%) at 120 minutes after administration from in vivo fluorescence imaging. Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

No MeSH data available.


Related in: MedlinePlus

Quantification of liposomes release by mitigation of fluorescein self-quench.Notes: Fluorescein solution in self-quench concentration (75 mM) was encapsulated in liposome. After fiber-optic triggered photo-thermal responsive release, discharged fluorescein from liposome was diluted and emitted brighter fluorescence.Abbreviation: TOP, tri-n-octylphosphine.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4542555&req=5

f2-ijn-10-5171: Quantification of liposomes release by mitigation of fluorescein self-quench.Notes: Fluorescein solution in self-quench concentration (75 mM) was encapsulated in liposome. After fiber-optic triggered photo-thermal responsive release, discharged fluorescein from liposome was diluted and emitted brighter fluorescence.Abbreviation: TOP, tri-n-octylphosphine.

Mentions: The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes for topical release in vivo (in tumors of xenograft mice, Figure 1B) using a 200 μm optical fiber and a 65 mW continuous wave (CW) diode laser (Figure 1A). The photo-thermally reactive liposomes consisted of hydrophobic gold nanoparticles (AuNPs) tethered to the membrane of a temperature-responsive liposome; this design was responsible for converting light energy into heat by surface plasma resonance to increase liposome permeability when reaching the phase transition temperature.16,21,31 AuNPs are well recognized as biocompatible and have been applied in medical studies for decades, and thermal responsive liposomes are under clinical trials, such as 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcho-line (DPPC)-based Thermodox in Phase III.32,33 The pattern of triggered content release was quantified by an increase in fluorescence (in vivo fluorescence imaging) due to the dilution of a 75 mM (self-quench concentration) fluorescein solution when released from the liposome (Figure 2).34


Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy.

Huang HL, Lu PH, Yang HC, Lee GD, Li HR, Liao KC - Int J Nanomedicine (2015)

Quantification of liposomes release by mitigation of fluorescein self-quench.Notes: Fluorescein solution in self-quench concentration (75 mM) was encapsulated in liposome. After fiber-optic triggered photo-thermal responsive release, discharged fluorescein from liposome was diluted and emitted brighter fluorescence.Abbreviation: TOP, tri-n-octylphosphine.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542555&req=5

f2-ijn-10-5171: Quantification of liposomes release by mitigation of fluorescein self-quench.Notes: Fluorescein solution in self-quench concentration (75 mM) was encapsulated in liposome. After fiber-optic triggered photo-thermal responsive release, discharged fluorescein from liposome was diluted and emitted brighter fluorescence.Abbreviation: TOP, tri-n-octylphosphine.
Mentions: The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes for topical release in vivo (in tumors of xenograft mice, Figure 1B) using a 200 μm optical fiber and a 65 mW continuous wave (CW) diode laser (Figure 1A). The photo-thermally reactive liposomes consisted of hydrophobic gold nanoparticles (AuNPs) tethered to the membrane of a temperature-responsive liposome; this design was responsible for converting light energy into heat by surface plasma resonance to increase liposome permeability when reaching the phase transition temperature.16,21,31 AuNPs are well recognized as biocompatible and have been applied in medical studies for decades, and thermal responsive liposomes are under clinical trials, such as 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcho-line (DPPC)-based Thermodox in Phase III.32,33 The pattern of triggered content release was quantified by an increase in fluorescence (in vivo fluorescence imaging) due to the dilution of a 75 mM (self-quench concentration) fluorescein solution when released from the liposome (Figure 2).34

Bottom Line: The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes.In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements.Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan.

ABSTRACT
The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes embedded with gold nanoparticles (AuNPs) using a 200 μm fiber with 65 mW and 532 nm excitation for topical release in vivo. The tunable delivery function can be paired with an apoptosis biosensor based on the same fiber-optic configuration for providing real-time evaluation of chemotherapy efficacy in vivo to perform as a personalized chemotherapy system. The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes. In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements. Using the mouse xenograft studies, we first demonstrated that the encapsulation of fluorescein in liposomes resulted in a more substantial content retention (81%) in the tumor than for free fluorophores (14%) at 120 minutes after administration from in vivo fluorescence imaging. Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

No MeSH data available.


Related in: MedlinePlus