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Detecting microvascular changes in the mouse spleen using optical computed tomography.

McErlean CM, Boult JK, Collins DJ, Leach MO, Robinson SP, Doran SJ - Microvasc. Res. (2015)

Bottom Line: A significant difference in total splenic volume was found between vehicle and ZD6126-treated cohorts, with mean volumes of 61±3mm(3) and 44±3mm(3) respectively (both n=3, p=0.05).Textural statistics for each sample were calculated using grey-level co-occurrence matrices (GLCMs).Standard 2-D GLCM analysis was found to be slice-dependent while 3-D GLCM contrast and homogeneity analysis resulted in separation of the vehicle and ZD6126-treated cohorts over a range of length scales.

View Article: PubMed Central - PubMed

Affiliation: Cancer Research UK Cancer Imaging Centre, The Institute of Cancer Research, Sutton SM2 5NG, UK. Electronic address: cmcerlean@icr.ac.uk.

No MeSH data available.


Related in: MedlinePlus

a. A still image from a volume rendering of a ZD6126-treated spleen from Dataset 2 with FOV (5.3 mm)3, rendering made using OsiriX software (Rosset et al., 2004). b. A still view of orthogonal slices through a reconstructed volume of another ZD6126-treated spleen with FOV (5.3 mm)3. Full representations of these datasets are available online as video clips (see Appendix A). c. A magnified view of a single slice from the volume shown in b., with some anatomical features marked. RP: red pulp, MZ: marginal zone, BV: blood vessel, LV: lymph vessel, WP: white pulp. Note that blood is highly attenuating and appears bright on these scans. d. Total volume of vehicle and ZD6126-treated spleens (lines mark the mean for each group, *p = 0.05).
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f0020: a. A still image from a volume rendering of a ZD6126-treated spleen from Dataset 2 with FOV (5.3 mm)3, rendering made using OsiriX software (Rosset et al., 2004). b. A still view of orthogonal slices through a reconstructed volume of another ZD6126-treated spleen with FOV (5.3 mm)3. Full representations of these datasets are available online as video clips (see Appendix A). c. A magnified view of a single slice from the volume shown in b., with some anatomical features marked. RP: red pulp, MZ: marginal zone, BV: blood vessel, LV: lymph vessel, WP: white pulp. Note that blood is highly attenuating and appears bright on these scans. d. Total volume of vehicle and ZD6126-treated spleens (lines mark the mean for each group, *p = 0.05).

Mentions: High contrast data were successfully acquired for each spleen, and all animals were included in analysis. Fig. 3a. and b. display typical examples of the 3-D image datasets, in two complementary representations revealing the classical honeycomb appearance and complex inner structure. Full 3-D representations are available as supplementary video files. Fig. 3c. shows a single 2-D reconstructed slice annotated to show some anatomical features.


Detecting microvascular changes in the mouse spleen using optical computed tomography.

McErlean CM, Boult JK, Collins DJ, Leach MO, Robinson SP, Doran SJ - Microvasc. Res. (2015)

a. A still image from a volume rendering of a ZD6126-treated spleen from Dataset 2 with FOV (5.3 mm)3, rendering made using OsiriX software (Rosset et al., 2004). b. A still view of orthogonal slices through a reconstructed volume of another ZD6126-treated spleen with FOV (5.3 mm)3. Full representations of these datasets are available online as video clips (see Appendix A). c. A magnified view of a single slice from the volume shown in b., with some anatomical features marked. RP: red pulp, MZ: marginal zone, BV: blood vessel, LV: lymph vessel, WP: white pulp. Note that blood is highly attenuating and appears bright on these scans. d. Total volume of vehicle and ZD6126-treated spleens (lines mark the mean for each group, *p = 0.05).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542549&req=5

f0020: a. A still image from a volume rendering of a ZD6126-treated spleen from Dataset 2 with FOV (5.3 mm)3, rendering made using OsiriX software (Rosset et al., 2004). b. A still view of orthogonal slices through a reconstructed volume of another ZD6126-treated spleen with FOV (5.3 mm)3. Full representations of these datasets are available online as video clips (see Appendix A). c. A magnified view of a single slice from the volume shown in b., with some anatomical features marked. RP: red pulp, MZ: marginal zone, BV: blood vessel, LV: lymph vessel, WP: white pulp. Note that blood is highly attenuating and appears bright on these scans. d. Total volume of vehicle and ZD6126-treated spleens (lines mark the mean for each group, *p = 0.05).
Mentions: High contrast data were successfully acquired for each spleen, and all animals were included in analysis. Fig. 3a. and b. display typical examples of the 3-D image datasets, in two complementary representations revealing the classical honeycomb appearance and complex inner structure. Full 3-D representations are available as supplementary video files. Fig. 3c. shows a single 2-D reconstructed slice annotated to show some anatomical features.

Bottom Line: A significant difference in total splenic volume was found between vehicle and ZD6126-treated cohorts, with mean volumes of 61±3mm(3) and 44±3mm(3) respectively (both n=3, p=0.05).Textural statistics for each sample were calculated using grey-level co-occurrence matrices (GLCMs).Standard 2-D GLCM analysis was found to be slice-dependent while 3-D GLCM contrast and homogeneity analysis resulted in separation of the vehicle and ZD6126-treated cohorts over a range of length scales.

View Article: PubMed Central - PubMed

Affiliation: Cancer Research UK Cancer Imaging Centre, The Institute of Cancer Research, Sutton SM2 5NG, UK. Electronic address: cmcerlean@icr.ac.uk.

No MeSH data available.


Related in: MedlinePlus