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Detecting microvascular changes in the mouse spleen using optical computed tomography.

McErlean CM, Boult JK, Collins DJ, Leach MO, Robinson SP, Doran SJ - Microvasc. Res. (2015)

Bottom Line: A significant difference in total splenic volume was found between vehicle and ZD6126-treated cohorts, with mean volumes of 61±3mm(3) and 44±3mm(3) respectively (both n=3, p=0.05).Textural statistics for each sample were calculated using grey-level co-occurrence matrices (GLCMs).Standard 2-D GLCM analysis was found to be slice-dependent while 3-D GLCM contrast and homogeneity analysis resulted in separation of the vehicle and ZD6126-treated cohorts over a range of length scales.

View Article: PubMed Central - PubMed

Affiliation: Cancer Research UK Cancer Imaging Centre, The Institute of Cancer Research, Sutton SM2 5NG, UK. Electronic address: cmcerlean@icr.ac.uk.

No MeSH data available.


Related in: MedlinePlus

Diagram of the optical CT system used for imaging optically cleared spleen samples. Light passing through the sample was focused by a microscope lens system onto a camera chip and recorded in a ‘projection’ image for each rotation angle. See Doran et al. (2013) for details of individual components.
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f0010: Diagram of the optical CT system used for imaging optically cleared spleen samples. Light passing through the sample was focused by a microscope lens system onto a camera chip and recorded in a ‘projection’ image for each rotation angle. See Doran et al. (2013) for details of individual components.

Mentions: Imaging was performed using an in-house optical CT system, shown in Fig. 1, which was previously developed and well characterised for microbeam radiotherapy applications (Doran et al., 2013). Optical CT image reconstruction is similar to X-ray CT in that a series of ‘projection’ images are acquired, recording photon attenuation at different angles. Each sample was suspended from a sample holder and rotated 180° from above in a matching tank containing 1:2 BABB fluid, which has the same refractive index as the optically cleared samples. In order to achieve high resolution, a microscope zoom lens (Z16 APO zoom system, Leica Microsystems GmbH, Wetzlar, Germany) was used to focus each projection image onto a complementary metal-oxide semiconductor (CMOS) camera (Zyla sCMOS, Andor Technology PLC, Belfast, UK). Filtered backprojection was used to reconstruct axial slices through each sample from the many 2-D projection images.


Detecting microvascular changes in the mouse spleen using optical computed tomography.

McErlean CM, Boult JK, Collins DJ, Leach MO, Robinson SP, Doran SJ - Microvasc. Res. (2015)

Diagram of the optical CT system used for imaging optically cleared spleen samples. Light passing through the sample was focused by a microscope lens system onto a camera chip and recorded in a ‘projection’ image for each rotation angle. See Doran et al. (2013) for details of individual components.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542549&req=5

f0010: Diagram of the optical CT system used for imaging optically cleared spleen samples. Light passing through the sample was focused by a microscope lens system onto a camera chip and recorded in a ‘projection’ image for each rotation angle. See Doran et al. (2013) for details of individual components.
Mentions: Imaging was performed using an in-house optical CT system, shown in Fig. 1, which was previously developed and well characterised for microbeam radiotherapy applications (Doran et al., 2013). Optical CT image reconstruction is similar to X-ray CT in that a series of ‘projection’ images are acquired, recording photon attenuation at different angles. Each sample was suspended from a sample holder and rotated 180° from above in a matching tank containing 1:2 BABB fluid, which has the same refractive index as the optically cleared samples. In order to achieve high resolution, a microscope zoom lens (Z16 APO zoom system, Leica Microsystems GmbH, Wetzlar, Germany) was used to focus each projection image onto a complementary metal-oxide semiconductor (CMOS) camera (Zyla sCMOS, Andor Technology PLC, Belfast, UK). Filtered backprojection was used to reconstruct axial slices through each sample from the many 2-D projection images.

Bottom Line: A significant difference in total splenic volume was found between vehicle and ZD6126-treated cohorts, with mean volumes of 61±3mm(3) and 44±3mm(3) respectively (both n=3, p=0.05).Textural statistics for each sample were calculated using grey-level co-occurrence matrices (GLCMs).Standard 2-D GLCM analysis was found to be slice-dependent while 3-D GLCM contrast and homogeneity analysis resulted in separation of the vehicle and ZD6126-treated cohorts over a range of length scales.

View Article: PubMed Central - PubMed

Affiliation: Cancer Research UK Cancer Imaging Centre, The Institute of Cancer Research, Sutton SM2 5NG, UK. Electronic address: cmcerlean@icr.ac.uk.

No MeSH data available.


Related in: MedlinePlus