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Pseudomonas aeruginosa PAO1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance.

Periasamy S, Nair HA, Lee KW, Ong J, Goh JQ, Kjelleberg S, Rice SA - Front Microbiol (2015)

Bottom Line: In contrast, overproduction of alginate resulted in biofilms that were comprised of 35-40% of P. aeruginosa, which was significantly higher than the WT (5-20%).Loss of the Psl polysaccharide significantly reduced the percentage composition of P. aeruginosa in dual species biofilms with P. protegens (<1%).Thus, the polysaccharide composition of an individual species significantly impacts mixed species biofilm development and the emergent properties of such communities.

View Article: PubMed Central - PubMed

Affiliation: Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore Singapore.

ABSTRACT
Pseudomonas aeruginosa PAO1 produces three polysaccharides, alginate, Psl, and Pel that play distinct roles in attachment and biofilm formation for monospecies biofilms. Considerably less is known about their role in the development of mixed species biofilm communities. This study has investigated the roles of alginate, Psl, and Pel during biofilm formation of P. aeruginosa in a defined and experimentally informative mixed species biofilm community, consisting of P. aeruginosa, Pseudomonas protegens, and Klebsiella pneumoniae. Loss of the Psl polysaccharide had the biggest impact on the integration of P. aeruginosa in the mixed species biofilms, where the percent composition of the psl mutant was significantly lower (0.06%) than its wild-type (WT) parent (2.44%). In contrast, loss of the Pel polysaccharide had no impact on mixed species biofilm development. Loss of alginate or its overproduction resulted in P. aeruginosa representing 8.4 and 18.11%, respectively, of the mixed species biofilm. Dual species biofilms of P. aeruginosa and K. pneumoniae were not affected by loss of alginate, Pel, or Psl, while the mucoid P. aeruginosa strain achieved a greater biomass than its parent strain. When P. aeruginosa was grown with P. protegens, loss of the Pel or alginate polysaccharides resulted in biofilms that were not significantly different from biofilms formed by the WT PAO1. In contrast, overproduction of alginate resulted in biofilms that were comprised of 35-40% of P. aeruginosa, which was significantly higher than the WT (5-20%). Loss of the Psl polysaccharide significantly reduced the percentage composition of P. aeruginosa in dual species biofilms with P. protegens (<1%). Loss of the Psl polysaccharide significantly disrupted the communal stress resistance of the three species biofilms. Thus, the polysaccharide composition of an individual species significantly impacts mixed species biofilm development and the emergent properties of such communities.

No MeSH data available.


Related in: MedlinePlus

Spatial and temporal development of P. aeruginosa polysaccharide mutants grown with P. protegens as dual species biofilms. The proportion of the two species was calculated by quantitative image analysis. (A)P. aeruginosa WT, (B) Δalg, (C) ΔmucA, (D) Δpel, (E) Δpsl, and (F) biovolumes of P. aeruginosa and polysaccharide mutants. Statistical analysis was performed vs. the corresponding WT samples grown in parallel, which were very similar in all cases ***P < 0.001, ****P < 0.0001.
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Figure 2: Spatial and temporal development of P. aeruginosa polysaccharide mutants grown with P. protegens as dual species biofilms. The proportion of the two species was calculated by quantitative image analysis. (A)P. aeruginosa WT, (B) Δalg, (C) ΔmucA, (D) Δpel, (E) Δpsl, and (F) biovolumes of P. aeruginosa and polysaccharide mutants. Statistical analysis was performed vs. the corresponding WT samples grown in parallel, which were very similar in all cases ***P < 0.001, ****P < 0.0001.

Mentions: Similarly, the roles of the P. aeruginosa polysaccharides in mediating dual species biofilm interactions were also investigated. When grown as a dual species biofilm with P. protegens (Figure 2; Supplementary Figure S2) the mucA mutant showed a significant increase (35–40%) in relative biovolume compared to the WT P. aeruginosa (5–20%). There was no significant difference in the biovolumes for the pel and alg mutants relative to the WT. As observed for the three species biofilm, the psl mutant (<1% biovolume) was also severely impaired in its ability to establish a dual species biofilm with P. protegens.


Pseudomonas aeruginosa PAO1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance.

Periasamy S, Nair HA, Lee KW, Ong J, Goh JQ, Kjelleberg S, Rice SA - Front Microbiol (2015)

Spatial and temporal development of P. aeruginosa polysaccharide mutants grown with P. protegens as dual species biofilms. The proportion of the two species was calculated by quantitative image analysis. (A)P. aeruginosa WT, (B) Δalg, (C) ΔmucA, (D) Δpel, (E) Δpsl, and (F) biovolumes of P. aeruginosa and polysaccharide mutants. Statistical analysis was performed vs. the corresponding WT samples grown in parallel, which were very similar in all cases ***P < 0.001, ****P < 0.0001.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542536&req=5

Figure 2: Spatial and temporal development of P. aeruginosa polysaccharide mutants grown with P. protegens as dual species biofilms. The proportion of the two species was calculated by quantitative image analysis. (A)P. aeruginosa WT, (B) Δalg, (C) ΔmucA, (D) Δpel, (E) Δpsl, and (F) biovolumes of P. aeruginosa and polysaccharide mutants. Statistical analysis was performed vs. the corresponding WT samples grown in parallel, which were very similar in all cases ***P < 0.001, ****P < 0.0001.
Mentions: Similarly, the roles of the P. aeruginosa polysaccharides in mediating dual species biofilm interactions were also investigated. When grown as a dual species biofilm with P. protegens (Figure 2; Supplementary Figure S2) the mucA mutant showed a significant increase (35–40%) in relative biovolume compared to the WT P. aeruginosa (5–20%). There was no significant difference in the biovolumes for the pel and alg mutants relative to the WT. As observed for the three species biofilm, the psl mutant (<1% biovolume) was also severely impaired in its ability to establish a dual species biofilm with P. protegens.

Bottom Line: In contrast, overproduction of alginate resulted in biofilms that were comprised of 35-40% of P. aeruginosa, which was significantly higher than the WT (5-20%).Loss of the Psl polysaccharide significantly reduced the percentage composition of P. aeruginosa in dual species biofilms with P. protegens (<1%).Thus, the polysaccharide composition of an individual species significantly impacts mixed species biofilm development and the emergent properties of such communities.

View Article: PubMed Central - PubMed

Affiliation: Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore Singapore.

ABSTRACT
Pseudomonas aeruginosa PAO1 produces three polysaccharides, alginate, Psl, and Pel that play distinct roles in attachment and biofilm formation for monospecies biofilms. Considerably less is known about their role in the development of mixed species biofilm communities. This study has investigated the roles of alginate, Psl, and Pel during biofilm formation of P. aeruginosa in a defined and experimentally informative mixed species biofilm community, consisting of P. aeruginosa, Pseudomonas protegens, and Klebsiella pneumoniae. Loss of the Psl polysaccharide had the biggest impact on the integration of P. aeruginosa in the mixed species biofilms, where the percent composition of the psl mutant was significantly lower (0.06%) than its wild-type (WT) parent (2.44%). In contrast, loss of the Pel polysaccharide had no impact on mixed species biofilm development. Loss of alginate or its overproduction resulted in P. aeruginosa representing 8.4 and 18.11%, respectively, of the mixed species biofilm. Dual species biofilms of P. aeruginosa and K. pneumoniae were not affected by loss of alginate, Pel, or Psl, while the mucoid P. aeruginosa strain achieved a greater biomass than its parent strain. When P. aeruginosa was grown with P. protegens, loss of the Pel or alginate polysaccharides resulted in biofilms that were not significantly different from biofilms formed by the WT PAO1. In contrast, overproduction of alginate resulted in biofilms that were comprised of 35-40% of P. aeruginosa, which was significantly higher than the WT (5-20%). Loss of the Psl polysaccharide significantly reduced the percentage composition of P. aeruginosa in dual species biofilms with P. protegens (<1%). Loss of the Psl polysaccharide significantly disrupted the communal stress resistance of the three species biofilms. Thus, the polysaccharide composition of an individual species significantly impacts mixed species biofilm development and the emergent properties of such communities.

No MeSH data available.


Related in: MedlinePlus