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TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration.

Yang H, Cao C, Wu C, Yuan C, Gu Q, Shi Q, Zou J - Sci Rep (2015)

Bottom Line: As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively.In vitro assays demonstrated that co-culturing of nucleus pulposus cells with bone marrow mesenchymal stem cells resulted in significantly higher levels of TGF-βl secretion.However, the NF-κB positive cells were significantly less than other two control groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.

ABSTRACT
Recent studies suggest that cell therapy may be an effective way to repair intervertebral disc degeneration. As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively. The objective of this study was to explore the effects of TGF-β1 during bone marrow mesenchymal stem cells-based therapy for disc degeneration. In vitro assays demonstrated that co-culturing of nucleus pulposus cells with bone marrow mesenchymal stem cells resulted in significantly higher levels of TGF-βl secretion. This increase inhibited IκB phosphorylation and NF-κB activation, detected by western blot analysis. Meanwhile, in a rabbit model, MRI analysis revealed significant recovery of signal intensity in the degenerative discs of rabbits receiving cells transplantation, than receiving cells treated with a TGF-β1 inhibitor or saline. These findings indicated that enhanced TGF-β1 production recovered the degeneration of intervertebral disc. And also immunohistochemical staining detected enhanced collagen II expression in the rabbits treated with cell transplantation. However, the NF-κB positive cells were significantly less than other two control groups. Thus, cell therapy promoted TGF-β1 expression in nucleus pulposus, leading to anti-inflammatory effects via the inhibition of NF-κB, and the amelioration of disc degradation due to increased expression of collagen II and aggrecan in degenerative intervertebral disc.

No MeSH data available.


Related in: MedlinePlus

Collagen II immunohistochemical staining (×200).Collagen II, which provides the extracellular matrix framework in cartilage tissue, was detected in the BMSCs transplantation group, but little in the two control groups.
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f6: Collagen II immunohistochemical staining (×200).Collagen II, which provides the extracellular matrix framework in cartilage tissue, was detected in the BMSCs transplantation group, but little in the two control groups.

Mentions: A normal nucleus pulposus contains a large number of nuclear cells. After 4 weeks, there was a decrease in the number of nuclear cells in the nucleus pulposus of the saline and SB431542 groups, and the nucleus pulposus became tissue-like fibrocartilage containing even fewer nuclear cells after 8 weeks. However, in the group receiving BMSCs transplantation, spindle cells formation was observed within the stent after 4 weeks, and there were many nuclear cells outside of the stents. Over the time course, the stent areas slowly decreased in size as the number of cells both within and around the stents declined. (Fig. 5). Collagen II, a typical marker, was detected in the nucleus pulposus tissue in BMSCs transplantation group greatly, but little to no collagen II was expressed in the two control groups. (Fig. 6).


TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration.

Yang H, Cao C, Wu C, Yuan C, Gu Q, Shi Q, Zou J - Sci Rep (2015)

Collagen II immunohistochemical staining (×200).Collagen II, which provides the extracellular matrix framework in cartilage tissue, was detected in the BMSCs transplantation group, but little in the two control groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542522&req=5

f6: Collagen II immunohistochemical staining (×200).Collagen II, which provides the extracellular matrix framework in cartilage tissue, was detected in the BMSCs transplantation group, but little in the two control groups.
Mentions: A normal nucleus pulposus contains a large number of nuclear cells. After 4 weeks, there was a decrease in the number of nuclear cells in the nucleus pulposus of the saline and SB431542 groups, and the nucleus pulposus became tissue-like fibrocartilage containing even fewer nuclear cells after 8 weeks. However, in the group receiving BMSCs transplantation, spindle cells formation was observed within the stent after 4 weeks, and there were many nuclear cells outside of the stents. Over the time course, the stent areas slowly decreased in size as the number of cells both within and around the stents declined. (Fig. 5). Collagen II, a typical marker, was detected in the nucleus pulposus tissue in BMSCs transplantation group greatly, but little to no collagen II was expressed in the two control groups. (Fig. 6).

Bottom Line: As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively.In vitro assays demonstrated that co-culturing of nucleus pulposus cells with bone marrow mesenchymal stem cells resulted in significantly higher levels of TGF-βl secretion.However, the NF-κB positive cells were significantly less than other two control groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.

ABSTRACT
Recent studies suggest that cell therapy may be an effective way to repair intervertebral disc degeneration. As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively. The objective of this study was to explore the effects of TGF-β1 during bone marrow mesenchymal stem cells-based therapy for disc degeneration. In vitro assays demonstrated that co-culturing of nucleus pulposus cells with bone marrow mesenchymal stem cells resulted in significantly higher levels of TGF-βl secretion. This increase inhibited IκB phosphorylation and NF-κB activation, detected by western blot analysis. Meanwhile, in a rabbit model, MRI analysis revealed significant recovery of signal intensity in the degenerative discs of rabbits receiving cells transplantation, than receiving cells treated with a TGF-β1 inhibitor or saline. These findings indicated that enhanced TGF-β1 production recovered the degeneration of intervertebral disc. And also immunohistochemical staining detected enhanced collagen II expression in the rabbits treated with cell transplantation. However, the NF-κB positive cells were significantly less than other two control groups. Thus, cell therapy promoted TGF-β1 expression in nucleus pulposus, leading to anti-inflammatory effects via the inhibition of NF-κB, and the amelioration of disc degradation due to increased expression of collagen II and aggrecan in degenerative intervertebral disc.

No MeSH data available.


Related in: MedlinePlus