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TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration.

Yang H, Cao C, Wu C, Yuan C, Gu Q, Shi Q, Zou J - Sci Rep (2015)

Bottom Line: As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively.In vitro assays demonstrated that co-culturing of nucleus pulposus cells with bone marrow mesenchymal stem cells resulted in significantly higher levels of TGF-βl secretion.However, the NF-κB positive cells were significantly less than other two control groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.

ABSTRACT
Recent studies suggest that cell therapy may be an effective way to repair intervertebral disc degeneration. As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively. The objective of this study was to explore the effects of TGF-β1 during bone marrow mesenchymal stem cells-based therapy for disc degeneration. In vitro assays demonstrated that co-culturing of nucleus pulposus cells with bone marrow mesenchymal stem cells resulted in significantly higher levels of TGF-βl secretion. This increase inhibited IκB phosphorylation and NF-κB activation, detected by western blot analysis. Meanwhile, in a rabbit model, MRI analysis revealed significant recovery of signal intensity in the degenerative discs of rabbits receiving cells transplantation, than receiving cells treated with a TGF-β1 inhibitor or saline. These findings indicated that enhanced TGF-β1 production recovered the degeneration of intervertebral disc. And also immunohistochemical staining detected enhanced collagen II expression in the rabbits treated with cell transplantation. However, the NF-κB positive cells were significantly less than other two control groups. Thus, cell therapy promoted TGF-β1 expression in nucleus pulposus, leading to anti-inflammatory effects via the inhibition of NF-κB, and the amelioration of disc degradation due to increased expression of collagen II and aggrecan in degenerative intervertebral disc.

No MeSH data available.


Related in: MedlinePlus

MRI findings after cell transplantation into the rabbit nucleus pulposus.T2 signal intensity in the nucleus pulposus of the BMSCs-injected discs was stronger than the discs of other two control groups.
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f4: MRI findings after cell transplantation into the rabbit nucleus pulposus.T2 signal intensity in the nucleus pulposus of the BMSCs-injected discs was stronger than the discs of other two control groups.

Mentions: To evaluated the potential of TGF-β1 in vivo, a rabbit model of BMSCs transplantation was demonstrated and assessed. Brief, two consecutive rabbit lumbar intervertebral discs were exposed and punctured using an 18G needle to induce disc degeneration. Four weeks after the initial puncture, either 1 × 106 BMSCs, BMSCs + TGF-βl inhibitor SB431542 or saline only were injected. A sagittal T2-weighted image was obtained to establish the positions of lumbar discs from L4-5 and L5-6. There were significant decreases in the MRI T2-weighted signal intensities shortly after the induction of disc degeneration in the SB431542 and saline groups. Signal intensities were only restored in the BMSCs transplantation group post-transplantation. MRI of the rabbit nucleus pulposus in the BMSCs transplantation group showed stronger signal intensities than those in the control and the TGF-β1 specific inhibitor-treated group at both 4 and 8 weeks after injection. (Fig. 4).


TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration.

Yang H, Cao C, Wu C, Yuan C, Gu Q, Shi Q, Zou J - Sci Rep (2015)

MRI findings after cell transplantation into the rabbit nucleus pulposus.T2 signal intensity in the nucleus pulposus of the BMSCs-injected discs was stronger than the discs of other two control groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542522&req=5

f4: MRI findings after cell transplantation into the rabbit nucleus pulposus.T2 signal intensity in the nucleus pulposus of the BMSCs-injected discs was stronger than the discs of other two control groups.
Mentions: To evaluated the potential of TGF-β1 in vivo, a rabbit model of BMSCs transplantation was demonstrated and assessed. Brief, two consecutive rabbit lumbar intervertebral discs were exposed and punctured using an 18G needle to induce disc degeneration. Four weeks after the initial puncture, either 1 × 106 BMSCs, BMSCs + TGF-βl inhibitor SB431542 or saline only were injected. A sagittal T2-weighted image was obtained to establish the positions of lumbar discs from L4-5 and L5-6. There were significant decreases in the MRI T2-weighted signal intensities shortly after the induction of disc degeneration in the SB431542 and saline groups. Signal intensities were only restored in the BMSCs transplantation group post-transplantation. MRI of the rabbit nucleus pulposus in the BMSCs transplantation group showed stronger signal intensities than those in the control and the TGF-β1 specific inhibitor-treated group at both 4 and 8 weeks after injection. (Fig. 4).

Bottom Line: As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively.In vitro assays demonstrated that co-culturing of nucleus pulposus cells with bone marrow mesenchymal stem cells resulted in significantly higher levels of TGF-βl secretion.However, the NF-κB positive cells were significantly less than other two control groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.

ABSTRACT
Recent studies suggest that cell therapy may be an effective way to repair intervertebral disc degeneration. As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively. The objective of this study was to explore the effects of TGF-β1 during bone marrow mesenchymal stem cells-based therapy for disc degeneration. In vitro assays demonstrated that co-culturing of nucleus pulposus cells with bone marrow mesenchymal stem cells resulted in significantly higher levels of TGF-βl secretion. This increase inhibited IκB phosphorylation and NF-κB activation, detected by western blot analysis. Meanwhile, in a rabbit model, MRI analysis revealed significant recovery of signal intensity in the degenerative discs of rabbits receiving cells transplantation, than receiving cells treated with a TGF-β1 inhibitor or saline. These findings indicated that enhanced TGF-β1 production recovered the degeneration of intervertebral disc. And also immunohistochemical staining detected enhanced collagen II expression in the rabbits treated with cell transplantation. However, the NF-κB positive cells were significantly less than other two control groups. Thus, cell therapy promoted TGF-β1 expression in nucleus pulposus, leading to anti-inflammatory effects via the inhibition of NF-κB, and the amelioration of disc degradation due to increased expression of collagen II and aggrecan in degenerative intervertebral disc.

No MeSH data available.


Related in: MedlinePlus