Limits...
Technique of porcine liver procurement and orthotopic transplantation using an active porto-caval shunt.

Spetzler VN, Goldaracena N, Knaak JM, Louis KS, Selzner N, Selzner M - J Vis Exp (2015)

Bottom Line: Decompression of intestinal congestion during the anhepatic phase of porcine OLTx is important to guarantee reliable animal survival.The use of an active porto-caval-jugular shunt achieves excellent intestinal decompression.The system can be used for short-term as well as long-term survival experiments.

View Article: PubMed Central - PubMed

Affiliation: Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital.

ABSTRACT
The success of liver transplantation has resulted in a dramatic organ shortage. Each year, a considerable number of patients on the liver transplantation waiting list die without receiving an organ transplant or are delisted due to disease progression. Even after a successful transplantation, rejection and side effects of immunosuppression remain major concerns for graft survival and patient morbidity. Experimental animal research has been essential to the success of liver transplantation and still plays a pivotal role in the development of clinical transplantation practice. In particular, the porcine orthotopic liver transplantation model (OLTx) is optimal for clinically oriented research for its close resemblance to human size, anatomy, and physiology. Decompression of intestinal congestion during the anhepatic phase of porcine OLTx is important to guarantee reliable animal survival. The use of an active porto-caval-jugular shunt achieves excellent intestinal decompression. The system can be used for short-term as well as long-term survival experiments. The following protocol contains all technical information for a stable and reproducible liver transplantation model in pigs including post-operative animal care.

Show MeSH

Related in: MedlinePlus

Play Video
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4542501&req=5


Technique of porcine liver procurement and orthotopic transplantation using an active porto-caval shunt.

Spetzler VN, Goldaracena N, Knaak JM, Louis KS, Selzner N, Selzner M - J Vis Exp (2015)

© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4542501&req=5

Bottom Line: Decompression of intestinal congestion during the anhepatic phase of porcine OLTx is important to guarantee reliable animal survival.The use of an active porto-caval-jugular shunt achieves excellent intestinal decompression.The system can be used for short-term as well as long-term survival experiments.

View Article: PubMed Central - PubMed

Affiliation: Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital.

ABSTRACT
The success of liver transplantation has resulted in a dramatic organ shortage. Each year, a considerable number of patients on the liver transplantation waiting list die without receiving an organ transplant or are delisted due to disease progression. Even after a successful transplantation, rejection and side effects of immunosuppression remain major concerns for graft survival and patient morbidity. Experimental animal research has been essential to the success of liver transplantation and still plays a pivotal role in the development of clinical transplantation practice. In particular, the porcine orthotopic liver transplantation model (OLTx) is optimal for clinically oriented research for its close resemblance to human size, anatomy, and physiology. Decompression of intestinal congestion during the anhepatic phase of porcine OLTx is important to guarantee reliable animal survival. The use of an active porto-caval-jugular shunt achieves excellent intestinal decompression. The system can be used for short-term as well as long-term survival experiments. The following protocol contains all technical information for a stable and reproducible liver transplantation model in pigs including post-operative animal care.

Show MeSH
Related in: MedlinePlus