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Integrating Paper Chromatography with Electrochemical Detection for the Trace Analysis of TNT in Soil.

Ryan P, Zabetakis D, Stenger DA, Trammell SA - Sensors (Basel) (2015)

Bottom Line: The probe is a combination of graphite electrodes, filter paper, with ethylene glycol and choline chloride as the solvent/electrolyte.Square wave chromatovoltammograms show the probes have a sensitivity for TNT of 0.75 nA/ng and a limit of detection of 100 ng.In addition, by taking advantage of the inherent paper chromatography step, TNT can be separated in both time and cathodic peak potential from 4-amino-dinitrotolene co-spotted on the probe or in soil samples with the presence of methyl parathion as a possible interferent.

View Article: PubMed Central - PubMed

Affiliation: Science and Engineering Apprenticeship Program, Naval Research Laboratory, Washington, DC 20375, USA. patrick_ryan_@outlook.com.

ABSTRACT
We report on the development of an electrochemical probe for the trace analysis of 2,4,6-trinitrotoluene (TNT) in soil samples. The probe is a combination of graphite electrodes, filter paper, with ethylene glycol and choline chloride as the solvent/electrolyte. Square wave chromatovoltammograms show the probes have a sensitivity for TNT of 0.75 nA/ng and a limit of detection of 100 ng. In addition, by taking advantage of the inherent paper chromatography step, TNT can be separated in both time and cathodic peak potential from 4-amino-dinitrotolene co-spotted on the probe or in soil samples with the presence of methyl parathion as a possible interferent.

No MeSH data available.


Related in: MedlinePlus

Separation of 5 µg of 4-Am-DNT and TNT placed on the paper-based electrochemical microelectrode (A) Square wave voltammograms at different time points; (B) Time traces at two different potentials; (C,D) 3D square wave chromatovoltammograms. Parameters: Amplitude = 25 mV, Frequency = 60 Hz.
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sensors-15-17048-f004: Separation of 5 µg of 4-Am-DNT and TNT placed on the paper-based electrochemical microelectrode (A) Square wave voltammograms at different time points; (B) Time traces at two different potentials; (C,D) 3D square wave chromatovoltammograms. Parameters: Amplitude = 25 mV, Frequency = 60 Hz.

Mentions: We chose to examine a 1:1 mixture of TNT and 4-Am-DNT to characterize the ability of the paper to separate similar compounds. 4-Am-DNT is a decomposition product of TNT and is found in soil of contaminated sites. In the experiment, 1000 ng of TNT and 4-Am-DNT were co-spotted on the paper and a chromatovoltammogram was generated as before. The resulting chromatovoltammograms are shown in Figure 4B plotting the peak maximum at −0.3 V and −0.5 V vs. Ag/AgCl vs. time. A 3D plot is shown in Figure 4C,D.


Integrating Paper Chromatography with Electrochemical Detection for the Trace Analysis of TNT in Soil.

Ryan P, Zabetakis D, Stenger DA, Trammell SA - Sensors (Basel) (2015)

Separation of 5 µg of 4-Am-DNT and TNT placed on the paper-based electrochemical microelectrode (A) Square wave voltammograms at different time points; (B) Time traces at two different potentials; (C,D) 3D square wave chromatovoltammograms. Parameters: Amplitude = 25 mV, Frequency = 60 Hz.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541921&req=5

sensors-15-17048-f004: Separation of 5 µg of 4-Am-DNT and TNT placed on the paper-based electrochemical microelectrode (A) Square wave voltammograms at different time points; (B) Time traces at two different potentials; (C,D) 3D square wave chromatovoltammograms. Parameters: Amplitude = 25 mV, Frequency = 60 Hz.
Mentions: We chose to examine a 1:1 mixture of TNT and 4-Am-DNT to characterize the ability of the paper to separate similar compounds. 4-Am-DNT is a decomposition product of TNT and is found in soil of contaminated sites. In the experiment, 1000 ng of TNT and 4-Am-DNT were co-spotted on the paper and a chromatovoltammogram was generated as before. The resulting chromatovoltammograms are shown in Figure 4B plotting the peak maximum at −0.3 V and −0.5 V vs. Ag/AgCl vs. time. A 3D plot is shown in Figure 4C,D.

Bottom Line: The probe is a combination of graphite electrodes, filter paper, with ethylene glycol and choline chloride as the solvent/electrolyte.Square wave chromatovoltammograms show the probes have a sensitivity for TNT of 0.75 nA/ng and a limit of detection of 100 ng.In addition, by taking advantage of the inherent paper chromatography step, TNT can be separated in both time and cathodic peak potential from 4-amino-dinitrotolene co-spotted on the probe or in soil samples with the presence of methyl parathion as a possible interferent.

View Article: PubMed Central - PubMed

Affiliation: Science and Engineering Apprenticeship Program, Naval Research Laboratory, Washington, DC 20375, USA. patrick_ryan_@outlook.com.

ABSTRACT
We report on the development of an electrochemical probe for the trace analysis of 2,4,6-trinitrotoluene (TNT) in soil samples. The probe is a combination of graphite electrodes, filter paper, with ethylene glycol and choline chloride as the solvent/electrolyte. Square wave chromatovoltammograms show the probes have a sensitivity for TNT of 0.75 nA/ng and a limit of detection of 100 ng. In addition, by taking advantage of the inherent paper chromatography step, TNT can be separated in both time and cathodic peak potential from 4-amino-dinitrotolene co-spotted on the probe or in soil samples with the presence of methyl parathion as a possible interferent.

No MeSH data available.


Related in: MedlinePlus