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Application of Visible and Near-Infrared Hyperspectral Imaging to Determine Soluble Protein Content in Oilseed Rape Leaves.

Zhang C, Liu F, Kong W, He Y - Sensors (Basel) (2015)

Bottom Line: SPA-PLS model obtained the best performance with r(p) of 0.9554, RMSEP of 0.1538 mg/g and RPD of 3.25.Distribution of protein content within the rape leaves were visualized and mapped on the basis of the SPA-PLS model.The overall results indicated that hyperspectral imaging could be used to determine and visualize the soluble protein content of rape leaves.

View Article: PubMed Central - PubMed

Affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China. chuzh@zju.edu.cn.

ABSTRACT
Visible and near-infrared hyperspectral imaging covering spectral range of 380-1030 nm as a rapid and non-destructive method was applied to estimate the soluble protein content of oilseed rape leaves. Average spectrum (500-900 nm) of the region of interest (ROI) of each sample was extracted, and four samples out of 128 samples were defined as outliers by Monte Carlo-partial least squares (MCPLS). Partial least squares (PLS) model using full spectra obtained dependable performance with the correlation coefficient (r(p)) of 0.9441, root mean square error of prediction (RMSEP) of 0.1658 mg/g and residual prediction deviation (RPD) of 2.98. The weighted regression coefficient (Bw), successive projections algorithm (SPA) and genetic algorithm-partial least squares (GAPLS) selected 18, 15, and 16 sensitive wavelengths, respectively. SPA-PLS model obtained the best performance with r(p) of 0.9554, RMSEP of 0.1538 mg/g and RPD of 3.25. Distribution of protein content within the rape leaves were visualized and mapped on the basis of the SPA-PLS model. The overall results indicated that hyperspectral imaging could be used to determine and visualize the soluble protein content of rape leaves.

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The schematic diagram of the hyperspectral imaging system.
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sensors-15-16576-f001: The schematic diagram of the hyperspectral imaging system.

Mentions: A visible and near-infrared hyperspectral imaging system including the following devices was used: an imaging spectrograph (ImSpector V10E; Spectral Imaging Ltd., Oulu, Finland) covering the spectral range of 380–1030 nm with the spectral resolution of 2.8 nm, a high performance CCD camera (Hamamatsu, Hamamatsu City, Japan) with 672 × 512 (spatial × spectral) pixels, a camera lens (OLES23; Specim, Spectral Imaging Ltd., Oulu, Finland), two 150 W tungsten halogen lamps (Fiber-Lite DC950 Illuminator; Dolan Jenner Industries Inc., Boxborough, MA, USA) for illumination, a conveyer belt driven by a stepper motor (Isuzu Optics Corp, Taiwan, China). All these devices were integrated as the hyperspectral imaging system (Figure 1) and the hyperspectral imaging system was controlled by a data acquisition and system control software (Spectral Image-V10E, Isuzu Optics Corp, Taiwan, China). The samples were scanned line by line along the Y-axis with the sample moving along the X-axis at a certain speed to obtain a three-dimensional hypercube.


Application of Visible and Near-Infrared Hyperspectral Imaging to Determine Soluble Protein Content in Oilseed Rape Leaves.

Zhang C, Liu F, Kong W, He Y - Sensors (Basel) (2015)

The schematic diagram of the hyperspectral imaging system.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541894&req=5

sensors-15-16576-f001: The schematic diagram of the hyperspectral imaging system.
Mentions: A visible and near-infrared hyperspectral imaging system including the following devices was used: an imaging spectrograph (ImSpector V10E; Spectral Imaging Ltd., Oulu, Finland) covering the spectral range of 380–1030 nm with the spectral resolution of 2.8 nm, a high performance CCD camera (Hamamatsu, Hamamatsu City, Japan) with 672 × 512 (spatial × spectral) pixels, a camera lens (OLES23; Specim, Spectral Imaging Ltd., Oulu, Finland), two 150 W tungsten halogen lamps (Fiber-Lite DC950 Illuminator; Dolan Jenner Industries Inc., Boxborough, MA, USA) for illumination, a conveyer belt driven by a stepper motor (Isuzu Optics Corp, Taiwan, China). All these devices were integrated as the hyperspectral imaging system (Figure 1) and the hyperspectral imaging system was controlled by a data acquisition and system control software (Spectral Image-V10E, Isuzu Optics Corp, Taiwan, China). The samples were scanned line by line along the Y-axis with the sample moving along the X-axis at a certain speed to obtain a three-dimensional hypercube.

Bottom Line: SPA-PLS model obtained the best performance with r(p) of 0.9554, RMSEP of 0.1538 mg/g and RPD of 3.25.Distribution of protein content within the rape leaves were visualized and mapped on the basis of the SPA-PLS model.The overall results indicated that hyperspectral imaging could be used to determine and visualize the soluble protein content of rape leaves.

View Article: PubMed Central - PubMed

Affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China. chuzh@zju.edu.cn.

ABSTRACT
Visible and near-infrared hyperspectral imaging covering spectral range of 380-1030 nm as a rapid and non-destructive method was applied to estimate the soluble protein content of oilseed rape leaves. Average spectrum (500-900 nm) of the region of interest (ROI) of each sample was extracted, and four samples out of 128 samples were defined as outliers by Monte Carlo-partial least squares (MCPLS). Partial least squares (PLS) model using full spectra obtained dependable performance with the correlation coefficient (r(p)) of 0.9441, root mean square error of prediction (RMSEP) of 0.1658 mg/g and residual prediction deviation (RPD) of 2.98. The weighted regression coefficient (Bw), successive projections algorithm (SPA) and genetic algorithm-partial least squares (GAPLS) selected 18, 15, and 16 sensitive wavelengths, respectively. SPA-PLS model obtained the best performance with r(p) of 0.9554, RMSEP of 0.1538 mg/g and RPD of 3.25. Distribution of protein content within the rape leaves were visualized and mapped on the basis of the SPA-PLS model. The overall results indicated that hyperspectral imaging could be used to determine and visualize the soluble protein content of rape leaves.

Show MeSH
Related in: MedlinePlus