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Application of 3D Printing Technology in Increasing the Diagnostic Performance of Enzyme-Linked Immunosorbent Assay (ELISA) for Infectious Diseases.

Singh H, Shimojima M, Shiratori T, An le V, Sugamata M, Yang M - Sensors (Basel) (2015)

Bottom Line: Enzyme-linked Immunosorbent Assay (ELISA)-based diagnosis is the mainstay for measuring antibody response in infectious diseases and to support pathogen identification of potential use in infectious disease outbreaks and clinical care of individual patients.The development of laboratory diagnostics using readily available 3D printing technologies provides a timely opportunity for further expansion of this technology into immunodetection systems.Utilizing available 3D printing platforms, a '3D well' was designed and developed to have an increased surface area compared to those of 96-well plates.

View Article: PubMed Central - PubMed

Affiliation: Department of Intelligent Mechanical Systems, Graduate School of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo 191-0065, Japan. singhha3@gmail.com.

ABSTRACT
Enzyme-linked Immunosorbent Assay (ELISA)-based diagnosis is the mainstay for measuring antibody response in infectious diseases and to support pathogen identification of potential use in infectious disease outbreaks and clinical care of individual patients. The development of laboratory diagnostics using readily available 3D printing technologies provides a timely opportunity for further expansion of this technology into immunodetection systems. Utilizing available 3D printing platforms, a '3D well' was designed and developed to have an increased surface area compared to those of 96-well plates. The ease and rapidity of the development of the 3D well prototype provided an opportunity for its rapid validation through the diagnostic performance of ELISA in infectious disease without modifying current laboratory practices for ELISA. The improved sensitivity of the 3D well of up to 2.25-fold higher compared to the 96-well ELISA provides a potential for the expansion of this technology towards miniaturization and Lab-On-a-Chip platforms to reduce time, volume of reagents and samples needed for such assays in the laboratory diagnosis of infectious and other diseases including applications in other disciplines.

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Related in: MedlinePlus

Surface modification and ELISA efficiency for Rubella virus antibody at 1-point dilution of primary antibody (1:400) (OD405: optical density measured at 405 nm).
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sensors-15-16503-f003: Surface modification and ELISA efficiency for Rubella virus antibody at 1-point dilution of primary antibody (1:400) (OD405: optical density measured at 405 nm).

Mentions: Following the chemical etching method used in this study for modification of the 3D well to a hydrophilic surface, a 1-point dilution of primary antibody of 1:400 was carried out for IgG ELISA for Rubella virus antibody. These results, obtained from 3D wells following chemical etching, untreated 3D wells and 96-well plates are shown in Figure 3.


Application of 3D Printing Technology in Increasing the Diagnostic Performance of Enzyme-Linked Immunosorbent Assay (ELISA) for Infectious Diseases.

Singh H, Shimojima M, Shiratori T, An le V, Sugamata M, Yang M - Sensors (Basel) (2015)

Surface modification and ELISA efficiency for Rubella virus antibody at 1-point dilution of primary antibody (1:400) (OD405: optical density measured at 405 nm).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541890&req=5

sensors-15-16503-f003: Surface modification and ELISA efficiency for Rubella virus antibody at 1-point dilution of primary antibody (1:400) (OD405: optical density measured at 405 nm).
Mentions: Following the chemical etching method used in this study for modification of the 3D well to a hydrophilic surface, a 1-point dilution of primary antibody of 1:400 was carried out for IgG ELISA for Rubella virus antibody. These results, obtained from 3D wells following chemical etching, untreated 3D wells and 96-well plates are shown in Figure 3.

Bottom Line: Enzyme-linked Immunosorbent Assay (ELISA)-based diagnosis is the mainstay for measuring antibody response in infectious diseases and to support pathogen identification of potential use in infectious disease outbreaks and clinical care of individual patients.The development of laboratory diagnostics using readily available 3D printing technologies provides a timely opportunity for further expansion of this technology into immunodetection systems.Utilizing available 3D printing platforms, a '3D well' was designed and developed to have an increased surface area compared to those of 96-well plates.

View Article: PubMed Central - PubMed

Affiliation: Department of Intelligent Mechanical Systems, Graduate School of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo 191-0065, Japan. singhha3@gmail.com.

ABSTRACT
Enzyme-linked Immunosorbent Assay (ELISA)-based diagnosis is the mainstay for measuring antibody response in infectious diseases and to support pathogen identification of potential use in infectious disease outbreaks and clinical care of individual patients. The development of laboratory diagnostics using readily available 3D printing technologies provides a timely opportunity for further expansion of this technology into immunodetection systems. Utilizing available 3D printing platforms, a '3D well' was designed and developed to have an increased surface area compared to those of 96-well plates. The ease and rapidity of the development of the 3D well prototype provided an opportunity for its rapid validation through the diagnostic performance of ELISA in infectious disease without modifying current laboratory practices for ELISA. The improved sensitivity of the 3D well of up to 2.25-fold higher compared to the 96-well ELISA provides a potential for the expansion of this technology towards miniaturization and Lab-On-a-Chip platforms to reduce time, volume of reagents and samples needed for such assays in the laboratory diagnosis of infectious and other diseases including applications in other disciplines.

Show MeSH
Related in: MedlinePlus